LOCUS       Exported                4268 bp ds-DNA     circular SYN 21-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...o-c6p49nb
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 21, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /standard_name="C-term region_wako"
                     /label=C-term region_wako
     misc_feature    1..120
                     /standard_name="C-term region_wako compact"
                     /label=C-term region_wako compact
     misc_feature    142..729
                     /standard_name="Fok1"
                     /label=Fok1
     misc_recomb     complement(764..862)
                     /standard_name="attL4"
                     /label=attL4
     misc_recomb     764..787
                     /standard_name="attB4"
                     /label=attB4
     primer_bind     complement(887..906)
                     /standard_name="T7"
                     /label=T7
     primer_bind     complement(907..927)
                     /standard_name="M13-rev"
                     /label=M13-rev
     CDS             1040..1846
                     /standard_name="KanR"
                     /label=KanR
     rep_origin      1974..2602
     terminator      complement(2914..2941)
     terminator      complement(3073..3116)
     primer_bind     3182..3199
                     /standard_name="M13-fwd"
                     /label=M13-fwd
     misc_recomb     3216..3311
                     /standard_name="AttL1"
                     /label=AttL1
     CDS             3317..3343
                     /standard_name="HA tag"
                     /label=HA tag
     CDS             complement(3785..3853)
                     /standard_name="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     3924..3941
                     /standard_name="M13-fwd"
                     /label=M13-fwd
     primer_bind     3951..3970
                     /standard_name="T7"
                     /label=T7
     primer_bind     complement(4015..4034)
                     /standard_name="T3"
                     /label=T3
     primer_bind     complement(4052..4072)
                     /standard_name="M13-rev"
                     /label=M13-rev
     misc_binding    complement(4078..4100)
     promoter        complement(4105..4134)
                     /note="Geneious type: Promoter"
     misc_feature    4212..4268
                     /standard_name="HD_lastR_pt"
                     /label=HD_lastR_pt
ORIGIN
        1 tcaattgttg cccagttatc tcgccctgat ccggcgttgg ccgcgttgac caacgaccac
       61 ctcgtcgcct tggcctgcct cggcgggcgt cctgcgctgg atgcagtgaa aaagggattg
      121 ggggatccta tcagccgttc ccagctggtg aagtccgagc tggaggagaa gaaatccgag
      181 ttgaggcaca agctgaagta cgtgccccac gagtacatcg agctgatcga gatcgcccgg
      241 aacagcaccc aggaccgtat cctggagatg aaggtgatgg agttcttcat gaaggtgtac
      301 ggctacaggg gcaagcacct gggcggctcc aggaagcccg acggcgccat ctacaccgtg
      361 ggctccccca tcgactacgg cgtgatcgtg gacaccaagg cctactccgg cggctacaac
      421 ctgcccatcg gccaggccga cgaaatgcag aggtacgtgg aggagaacca gaccaggaac
      481 aagcacatca accccaacga gtggtggaag gtgtacccct ccagcgtgac cgagttcaag
      541 ttcctgttcg tgtccggcca cttcaagggc aactacaagg cccagctgac caggctgaac
      601 cacatcacca actgcaacgg cgccgtgctg tccgtggagg agctcctgat cggcggcgag
      661 atgatcaagg ccggcaccct gaccctggag gaggtgagga ggaagttcaa caacggcgag
      721 atcaacttcg cggccgactg ataactcgag cgatcctcta gacacccaac ttttctatac
      781 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
      841 agtcaaaata aaatcattat ttgccatcca gctgcagggc ggccgcccct atagtgagtc
      901 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
      961 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     1021 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     1081 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     1141 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1201 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     1261 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1321 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     1381 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     1441 ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctt gctcaggcgc aatcacgaat
     1501 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1561 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     1621 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     1681 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     1741 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     1801 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     1861 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     1921 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     1981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2761 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2821 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg
     2881 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
     2941 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
     3001 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
     3061 gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga
     3121 tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg
     3181 ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat
     3241 agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta
     3301 caaaaaagca ggcttttacc catacgatgt tccagattac gctgatatcg ccgatctacg
     3361 cacgctcggc tacagccagc agcaacagga gaagatcaaa ccgaaggttc gttcgacagt
     3421 ggcgcagcac cacgaggcac tggtcggcca cgggtttaca cacgcgcaca tcgttgcgtt
     3481 aagccaacac ccggcagcgt tagggaccgt cgctgtcaag tatcaggaca tgatcgcagc
     3541 gttgccagag gcgacacacg aagcgatcgt tggcgtcggc aaacagtggt ccggcgcacg
     3601 cgctctggag gccttgctca cggtggcggg agagttgaga ggtccaccgt tacagttgga
     3661 cacaggccaa cttctcaaga ttgcaaaacg tggcggcgtg accgcagtgg aggcagtgca
     3721 tgcatggcgc aatgcactga cgggtgcccc gctcaaggag acgggcgccg ctacagggcg
     3781 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     3841 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     3901 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     3961 cactataggg cgaattgggt accgggcccc ccctcgaggc cctccagctt ttgttccctt
     4021 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4081 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4141 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccac
     4201 cggtcgtctc cacccctgag caggtagtgg ctattgcatc ccacgacggg ggcagacccg
     4261 cactggag
//
