LOCUS       Exported                4268 bp ds-DNA     circular SYN 21-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...j-7twiatv
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 21, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..57
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1..57
                     /standard_name="lastNI"
                     /label=lastNI
     misc_feature    58..198
                     /standard_name="C-term region_wako"
                     /label=C-term region_wako
     misc_feature    58..177
                     /standard_name="C-term region_wako compact"
                     /label=C-term region_wako compact
     misc_feature    199..786
                     /standard_name="Fok1"
                     /label=Fok1
     misc_recomb     complement(821..919)
                     /standard_name="attL4"
                     /label=attL4
     misc_recomb     821..844
                     /standard_name="attB4"
                     /label=attB4
     primer_bind     complement(944..963)
                     /standard_name="T7"
                     /label=T7
     primer_bind     complement(964..984)
                     /standard_name="M13-rev"
                     /label=M13-rev
     CDS             1097..1903
                     /standard_name="KanR"
                     /label=KanR
     rep_origin      2031..2659
     terminator      complement(2971..2998)
     terminator      complement(3130..3173)
     primer_bind     3239..3256
                     /standard_name="M13-fwd"
                     /label=M13-fwd
     misc_recomb     3273..3368
                     /standard_name="AttL1"
                     /label=AttL1
     CDS             3374..3400
                     /standard_name="HA tag"
                     /label=HA tag
     CDS             complement(3842..3910)
                     /standard_name="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     3981..3998
                     /standard_name="M13-fwd"
                     /label=M13-fwd
     primer_bind     4008..4027
                     /standard_name="T7"
                     /label=T7
     primer_bind     complement(4072..4091)
                     /standard_name="T3"
                     /label=T3
     primer_bind     complement(4109..4129)
                     /standard_name="M13-rev"
                     /label=M13-rev
     misc_binding    complement(4135..4157)
     promoter        complement(4162..4191)
                     /note="Geneious type: Promoter"
     misc_feature    4268
                     /standard_name="ACCCCTGAGCAGGTAGTGGCTATTGCATCCCACGACGGGGGC 
                     AGACCCGCACTGGAG"
                     /label=Geneious type: Editing History Deletion
                     /note="Geneious type: Editing History Deletion"
ORIGIN
        1 acccctgagc aggtagtggc tattgcatcc aacatagggg gcagacccgc actggagtca
       61 attgttgccc agttatctcg ccctgatccg gcgttggccg cgttgaccaa cgaccacctc
      121 gtcgccttgg cctgcctcgg cgggcgtcct gcgctggatg cagtgaaaaa gggattgggg
      181 gatcctatca gccgttccca gctggtgaag tccgagctgg aggagaagaa atccgagttg
      241 aggcacaagc tgaagtacgt gccccacgag tacatcgagc tgatcgagat cgcccggaac
      301 agcacccagg accgtatcct ggagatgaag gtgatggagt tcttcatgaa ggtgtacggc
      361 tacaggggca agcacctggg cggctccagg aagcccgacg gcgccatcta caccgtgggc
      421 tcccccatcg actacggcgt gatcgtggac accaaggcct actccggcgg ctacaacctg
      481 cccatcggcc aggccgacga aatgcagagg tacgtggagg agaaccagac caggaacaag
      541 cacatcaacc ccaacgagtg gtggaaggtg tacccctcca gcgtgaccga gttcaagttc
      601 ctgttcgtgt ccggccactt caagggcaac tacaaggccc agctgaccag gctgaaccac
      661 atcaccaact gcaacggcgc cgtgctgtcc gtggaggagc tcctgatcgg cggcgagatg
      721 atcaaggccg gcaccctgac cctggaggag gtgaggagga agttcaacaa cggcgagatc
      781 aacttcgcgg ccgactgata actcgagcga tcctctagac acccaacttt tctatacaaa
      841 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      901 caaaataaaa tcattatttg ccatccagct gcagggcggc cgcccctata gtgagtcgta
      961 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     1021 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1081 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     1141 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1201 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1261 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1321 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1381 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1441 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1501 taattgtcct tttaacagcg atcgcgtatt tcgccttgct caggcgcaat cacgaatgaa
     1561 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1621 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     1681 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     1741 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     1801 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     1861 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     1921 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     1981 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     2041 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2101 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2161 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2221 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2281 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2341 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2401 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2461 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2521 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2581 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2641 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2701 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2761 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2821 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2881 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     2941 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     3001 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     3061 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     3121 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
     3181 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
     3241 taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt
     3301 gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa
     3361 aaaagcaggc ttttacccat acgatgttcc agattacgct gatatcgccg atctacgcac
     3421 gctcggctac agccagcagc aacaggagaa gatcaaaccg aaggttcgtt cgacagtggc
     3481 gcagcaccac gaggcactgg tcggccacgg gtttacacac gcgcacatcg ttgcgttaag
     3541 ccaacacccg gcagcgttag ggaccgtcgc tgtcaagtat caggacatga tcgcagcgtt
     3601 gccagaggcg acacacgaag cgatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc
     3661 tctggaggcc ttgctcacgg tggcgggaga gttgagaggt ccaccgttac agttggacac
     3721 aggccaactt ctcaagattg caaaacgtgg cggcgtgacc gcagtggagg cagtgcatgc
     3781 atggcgcaat gcactgacgg gtgccccgct caaggagacg ggcgccgcta cagggcgcgt
     3841 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3901 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     3961 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     4021 tatagggcga attgggtacc gggccccccc tcgaggccct ccagcttttg ttccctttag
     4081 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4141 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4201 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccaccgg
     4261 tcgtctcc
//
