LOCUS       pSMART                  2443 bp    DNA     circular     10-AUG-2020
FEATURES             Location/Qualifiers
     misc_feature    1946..2314
                     /note="GRFT"
     misc_feature    2315..2443
                     /note="Terminator"
     promoter        1789..1945
                     /dnas_title="yibD+rbs"
                     /note=""
     source          1..2443
                     /dnas_title="pSMART HCKan-Q-GRFT yibD"
ORIGIN      
        1 GACGAATTCT CTAGATATCG CTCAATACTG ACCATTTAAA TCATACCTGA CCTCCATAGC
       61 AGAAAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGAT CGGCACGTAA GAGGTTCCAA
      121 CTTTCACCAT AATGAAATAA GATCACTACC GGGCGTATTT TTTGAGTTAT CGAGATTTTC
      181 AGGAGCTAAG GAAGCTAAAA TGAGCCATAT TCAACGGGAA ACGTCTTGCT CGAGGCCGCG
      241 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG
      301 GCAATCAGGT GCGACAATCT ATCGATTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT
      361 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GGCTAAACTG
      421 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC
      481 ATGGTTACTC ACCACTGCGA TCCCAGGGAA AACAGCATTC CAGGTATTAG AAGAATATCC
      541 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT
      601 TCCTGTTTGT AATTGTCCTT TTAACGGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC
      661 ACGAATGAAT AACGGTTTGG TTGGTGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC
      721 TGTTGAACAA GTCTGGAAAG AAATGCATAA GCTTTTGCCA TTCTCACCGG ATTCAGTCGT
      781 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG
      841 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA
      901 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA
      961 TAATCCTGAT ATGAATAAAT TGCAGTTTCA CTTGATGCTC GATGAGTTTT TCTAATGAGG
     1021 GCCCAAATGT AATCACCTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTGC TGGCGTTTTT
     1081 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA TGCTCAAGTC AGAGGTGGCG
     1141 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1201 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1261 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1321 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1381 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1441 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1501 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTC
     1561 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
     1621 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATT
     1681 TTCTACCGAA GAAAGGCCCA CCCGTGAAGG TGAGCCAGTG AGTTGATTGC AGTCCAGTTA
     1741 CGCTGGAGTC TGAGGCTCGT CCTGAATGAT ATCAAGCTTG AATTCGTTGT GCGTAATTGT
     1801 GCTGATCTCT TATATAGCTG CTCTCATTAT CTCTCTACCC TGAAGTGACT CTCTCACCTG
     1861 TAAAAATAAT ATCTCACAGG CTTAATAGTT TCTTAATACA AAGCCTGTAA AACGTCAGGA
     1921 TAACTTCTGT GTAGGAGGAT AATCTATGTC TTTAACCCAC CGTAAATTTG GTGGATCCGG
     1981 TGGTTCACCT TTTAGTGGAT TATCGTCTAT TGCGGTGCGC TCTGGCTCGT ACTTAGATGC
     2041 AATCATCATT GATGGAGTAC ACCATGGCGG CTCCGGCGGC AACCTGTCGC CTACTTTCAC
     2101 TTTTGGCAGT GGGGAGTATA TTAGCAATAT GACCATCCGC TCGGGTGATT ATATTGACAA
     2161 TATCAGCTTT GAGACGAATC AGGGGCGTCG TTTCGGCCCA TACGGAGGGT CGGGTGGTTC
     2221 GGCTAATACG CTGTCCAATG TAAAGGTAAT CCAAATTAAT GGCTCCGCTG GCGACTATCT
     2281 TGATTCGTTG GACATTTATT ATGAACAATA TTGACCAGGC ATCAAATAAA ACGAAAGGCT
     2341 CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCTACTAG
     2401 AGTCACACTG GCTCACCTTC GGGTGGGCCT TTCTGCGTTT ATA
//

