LOCUS       Exported               10254 bp ds-DNA     circular SYN 27-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Jason Vevea
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 23, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2582..2624
                     /label=NC32 For IF
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(3030..3065)
                     /label=NC32 Rev IF
     misc_feature    3086..4039
                     /label=Synaptophysin
                     /note="Rat"
     misc_feature    3143..3214
                     /label=SYP TMD 1
     misc_feature    3389..3460
                     /label=SYP TMD 2
     misc_feature    3482..3553
                     /label=SYP TMD 3
     misc_feature    3668..3739
                     /label=SYP TMD 4
     misc_feature    3788..3817
                     /label=A'Q modified NS3 cleavage site
     misc_structure  4040..4240
                     /label=4x GS Linker
                     /note="67 amino acid flexible linker
                     Approx 23.5nm (theoretical) 
                     "
     misc_feature    4139..4240
                     /label=GS Linker 2x
     misc_feature    4199..4240
                     /label=GS(GSS)4 Linker 4
     misc_feature    4241..4852
                     /label=NS3/4 HCV FAST protease 1a *codon optimized*
                     /note="
                     
                     NS3/4a protease FAST VARIANT. Sequence based on 
                     PSD95-TS2-YFP but codon optimized.
                     
                     
                     Johnson, Owen, and Gale, "Functional and Therapeutic 
                     Analysis of Hepatitis C Virus NS3·4A Protease Control of 
                     Antiviral Immune Defense."  
                     
                     
                     Butko, M.T., Yang, J., Geng, Y., Kim, H.J., Jeon, N.L., 
                     Shu, X., Mackey, M.R., Ellisman, M.H., Tsien, R.Y. and Lin,
                     M.Z., 2012. Fluorescent and photo-oxidizing TimeSTAMP tags 
                     track protein fates in light and electron microscopy. 
                     Nature neuroscience, 15(12), pp.1742-1751."
     misc_feature    4241..4279
                     /label=NS4A alpha helix
                     /note="   Brass, V., Berke, J.M., Montserret, R., Blum, 
                     H.E., Penin, F., and Moradpour, D. (2008). Structural 
                     determinants for membrane association and dynamic 
                     organization of the hepatitis C virus NS3-4A complex. 
                     Proceedings of the National Academy of Sciences 105, 
                     14545?14550.    
                     
                     "
     misc_feature    4319..4363
                     /label=NS3 amphipathic helix a0
                     /note="   Brass, V., Berke, J.M., Montserret, R., Blum, 
                     H.E., Penin, F., and Moradpour, D. (2008). Structural 
                     determinants for membrane association and dynamic 
                     organization of the hepatitis C virus NS3-4A complex. 
                     Proceedings of the National Academy of Sciences 105, 
                     14545?14550.    
                     
                     "
     misc_feature    4853..4867
                     /label=NS3/4 HCV FAST protease 1a *codon optimized*
                     /note="
                     
                     NS3/4a protease FAST VARIANT. Sequence based on 
                     PSD95-TS2-YFP but codon optimized.
                     
                     
                     Johnson, Owen, and Gale, "Functional and Therapeutic 
                     Analysis of Hepatitis C Virus NS3·4A Protease Control of 
                     Antiviral Immune Defense."  
                     
                     
                     Butko, M.T., Yang, J., Geng, Y., Kim, H.J., Jeon, N.L., 
                     Shu, X., Mackey, M.R., Ellisman, M.H., Tsien, R.Y. and Lin,
                     M.Z., 2012. Fluorescent and photo-oxidizing TimeSTAMP tags 
                     track protein fates in light and electron microscopy. 
                     Nature neuroscience, 15(12), pp.1742-1751."
     misc_feature    4868..4903
                     /label=GS(GSS)4 Linker 3
     misc_feature    4870..4872
                     /label=GS(GSS)4 Linker 3
     CDS             4877..4900
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4946..5534
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5417..5428)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5537..5553)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6059..6239
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6271..6495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6541..6969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6983..7312
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7163..7298
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7360..7407
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7426..7800
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7930..8051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8100..8116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8124..8140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8148..8178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8193..8214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8502..9090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9261..10121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10122..10226)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatccggtac cgcgggcccg ccaccatgga cgtggtgaat cagctggtgg ctgggggtca
     3121 gttccgggtg gtcaaggagc cccttggctt cgtgaaggtg ctgcagtggg tctttgccat
     3181 cttcgccttt gctacgtgtg gcagctacac cggggagctt cggctgagcg tggagtgtgc
     3241 caacaagacg gagagtgccc tcaacatcga agttgaattc gagtacccct tcaggctgca
     3301 ccaagtgtac tttgatgcac cctcctgcgt caaagggggc actaccaaga tcttcctggt
     3361 tggggactac tcctcgtcgg ctgaattctt tgtcaccgtg gctgtgtttg ccttcctcta
     3421 ctccatgggg gccctggcca cctacatctt cctgcagaac aagtaccgag agaacaacaa
     3481 agggcctatg atggactttc tggctacagc cgtgttcgct ttcatgtggc tagttagttc
     3541 atcagcctgg gccaaaggcc tgtccgatgt gaagatggcc acggacccag agaacattat
     3601 caaggagatg cccatgtgcc gccagacagg gaacacatgc aaggaactga gggaccctgt
     3661 gacttcagga ctcaacacct cagtggtgtt tggcttcctg aacctggtgc tctgggttgg
     3721 caacttatgg ttcgtgttca aggagacagg ctgggcagcc ccattcatgc gcgcacctcc
     3781 aggcgccgct gacgtggttt gctgtcagat gagttatccg gaaaagcaac cagcacctgg
     3841 cgatgcctac ggcgatgcgg gctacgggca gggccccgga ggctatgggc cccaggactc
     3901 ctacgggcct cagggtggtt atcaacccga ttacgggcag ccagccagcg gtggcggtgg
     3961 ctacgggcct cagggcgact atgggcagca aggctatggc caacagggtg cgcccacctc
     4021 cttctccaat cagatgaaag gttcagggtc tagtggcagc tctggatcct cggggtcagc
     4081 aggcagtgct gccggttccg gaagcgggtc cagtggtagc tcaggctctt ccggaagcgg
     4141 atccggaagt tcggggtcca gcggttctag tggttcagcc ggaagcgccg ctggaagtgg
     4201 gtcaggatca tctggcagat cggggagtag cggttcctca ggctgcgtgg tgatcgtggg
     4261 caggatcgtg ctgagcggaa gcggcaccag cgcccccatc accgcctacg cccagcagac
     4321 caggggcctg ctgggctgca tcatcaccag cctgaccggc agagacaaga accaggtgga
     4381 gggcgaggtg cagatcgtga gcaccgccac ccagaccttc ctggccacct gcatcaacgg
     4441 agtgtgttgg accgtgtacc acggcgccgg caccagaacc atcgccagcc ccaagggccc
     4501 cgtgatccag atgtacacaa acgtggacca ggacctggtg ggctggcccg ccccccaggg
     4561 cagcaggagc ctgaccccct gcacatgcgg cagcagcgac ctgtacctgg ttaccaggca
     4621 cgccgacgtg atccccgtga ggaggagggg cgacagcagg ggcagcctgc tgagccccag
     4681 gcccatcagc tacttgaagg gatctagcgg aggccccctg ctgtgccccg ccggccacgc
     4741 cgtgggcctg ttcagggccg ccgtgtgcac caggggcgtg gccaaggccg tggacttcat
     4801 ccccgtggag aacctggaga ccaccatgag gagccccgtg tttaccgaca actcctctcc
     4861 accagcagga tccagtgact acaaggatga cgacgataaa taaggccgca ctcctcagct
     4921 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     4981 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5041 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5101 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5161 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5221 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5281 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5341 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5401 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5461 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5521 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     5581 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     5641 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     5701 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5761 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     5821 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     5881 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     5941 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6001 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     6061 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6121 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6181 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6241 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6301 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6361 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6421 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6481 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6541 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6601 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6661 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6721 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6781 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6841 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6901 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6961 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7021 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7081 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7141 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7201 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7261 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7321 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     7381 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     7441 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     7501 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     7561 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     7621 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7681 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7741 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7801 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7861 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7921 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8041 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8101 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8161 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8221 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8281 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8341 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8401 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8941 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9541 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9601 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9661 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9721 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9781 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9841 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9901 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9961 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10021 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10081 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10201 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
