LOCUS       Exported               10021 bp ds-DNA     circular SYN 23-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    D6.pcDNA5 3xFLAG EIF4G1 +MIC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 23, 2020 from SnapGene Viewer 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    209..819
                     /label=CMV promoter
                     /note="CMV promoter"
     misc_feature    820..859
                     /label=Tetracycline operator
                     /note="Tetracycline operator"
     misc_feature    989..1057
                     /label=3xFlag
                     /note="3xFlag"
     misc_feature    1058..1076
                     /label=attR1
                     /note="attR1"
     misc_feature    1085..5905
                     /label=EIF4G1
                     /note="EIF4G1"
     misc_feature    1229..1249
                     /label=MIC
                     /note="MIC"
     misc_feature    5910..5926
                     /label=attR2
                     /note="attR2"
     misc_feature    5963..6187
                     /label=BGH poly A
                     /note="BGH poly A"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttccaccat ggactacaaa gaccatgacg gtgatacata
     1021 tgatcatgac atcgattaca aggatgacga tgacaagaca agtttgtaca aaaaagcagg
     1081 cttcaacaaa gctccacagt ccacaggccc cccacccgcc ccatcccccg gactcccaca
     1141 gccagcgttt cccccggggc agacagcgcc ggtggtgttc agtacgccac aagcgacaca
     1201 aatgaacacg ccttctcagc cccgccaggg aggattcagg tctctgcagc acttctaccc
     1261 tagccgggcc cagcccccga gcagtgcagc ctcccgagtg cagagtgcag cccctgcccg
     1321 ccctggccca gctgcccatg tctaccctgc tggatcccaa gtaatgatga tcccttccca
     1381 gatctcctac ccagcctccc agggggccta ctacatccct ggacaggggc gttccacata
     1441 cgttgtcccg acacagcagt accctgtgca gccaggagcc ccaggcttct atccaggtgc
     1501 aagccctaca gaatttggga cctacgctgg cgcctactat ccagcccaag gggtgcagca
     1561 gtttcccact ggcgtggccc ccgccccagt tttgatgaac cagccacccc agattgctcc
     1621 caagagggag cgtaagacga tccgaattcg agatccaaac caaggaggaa aggatatcac
     1681 agaggagatc atgtctgggg cccgcactgc ctccacaccc acccctcccc agacgggagg
     1741 cggtctggag cctcaagcta atggggagac gccccaggtt gctgtcattg tccggccaga
     1801 tgaccggtca cagggagcaa tcattgctga ccggccaggg ctgcctggcc cagagcatag
     1861 cccttcagaa tcccagcctt cgtcgccttc tccgacccca tcaccatccc cagtcttgga
     1921 accggggtct gagcctaatc tcgcagtcct ctctattcct ggggacacta tgacaactat
     1981 acaaatgtct gtagaagaat caacccccat ctcccgtgaa actggggagc catatcgcct
     2041 ctctccagaa cccactcctc tcgccgaacc catactggaa gtagaagtga cacttagcaa
     2101 accggttcca gaatctgagt tttcttccag tcctctccag gctcccaccc ctttggcatc
     2161 tcacacagtg gaaattcatg agcctaatgg catggtccca tctgaagatc tggaaccaga
     2221 ggtggagtca agcccagagc ttgctcctcc cccagcttgc ccctccgaat cccctgtgcc
     2281 cattgctcca actgcccaac ctgaggaact gctcaacgga gccccctcgc caccagctgt
     2341 ggacttaagc ccagtcagtg agccagagga gcaggccaag gaggtgacag catcagtggc
     2401 gccccccacc atcccctctg ctactccagc tacggctcct tcagctactt ccccagctca
     2461 ggaggaggaa atggaagaag aagaagaaga ggaagaagga gaagcaggag aagcaggaga
     2521 agctgagagt gagaaaggag gagaggaact gctcccccca gagagtaccc ctattccagc
     2581 caacttgtct cagaatttgg aggcagcagc agccactcaa gtggcagtat ctgtgccaaa
     2641 gaggagacgg aaaattaagg agctaaataa gaaggaggct gttggagacc ttctggatgc
     2701 cttcaaggag gcgaacccgg cagtaccaga ggtggaaaat cagcctcctg caggcagcaa
     2761 tccaggccca gagtctgagg gcagtggtgt gcccccacgt cctgaggaag cagatgagac
     2821 ctgggactca aaggaagaca aaattcacaa tgctgagaac atccagcccg gggaacagaa
     2881 gtatgaatat aagtcagatc agtggaagcc tctaaaccta gaggagaaaa aacgttacga
     2941 ccgtgagttc ctgcttggtt ttcagttcat ctttgccagt atgcagaagc cagagggatt
     3001 gccacatatc agtgacgtgg tgctggacaa ggccaataaa acaccactgc ggccactgga
     3061 tcccactaga ctacaaggca taaattgtgg cccagacttc actccatcct ttgccaacct
     3121 tggccggaca acccttagca cccgtgggcc cccaaggggt gggccaggtg gggagctgcc
     3181 ccgtgggccg gctggcctgg gaccccggcg ctctcagcag ggaccccgaa aagaaccacg
     3241 caagatcatt gccacagtgt taatgaccga agatataaaa ctgaacaaag cagagaaagc
     3301 ctggaaaccc agcagcaagc ggacggcggc tgataaggat cgaggggaag aagatgctga
     3361 tggcagcaaa acccaggacc tattccgcag ggtgcgctcc atcctgaata aactgacacc
     3421 ccagatgttc cagcagctga tgaagcaagt gacgcagctg gccatcgaca ccgaggaacg
     3481 cctcaaaggg gtcattgacc tcatttttga gaaggccatt tcagagccca acttctctgt
     3541 ggcctatgcc aacatgtgcc gctgcctcat ggcgctgaaa gtgcccacta cggaaaagcc
     3601 aacagtgact gtgaacttcc gaaagctgtt gttgaatcga tgtcagaagg agtttgagaa
     3661 agacaaagat gatgatgagg tttttgagaa gaagcaaaaa gagatggatg aagctgctac
     3721 ggcagaggaa cgaggacgcc tgaaggaaga gctggaagag gctcgggaca tagcccggcg
     3781 gcgctcttta gggaatatca agtttattgg agagttgttc aaactgaaga tgttaacaga
     3841 ggcaataatg catgactgtg tggtcaaact gcttaagaac catgatgaag agtcccttga
     3901 gtgcctttgt cgtctgctca ccaccattgg caaagacctg gactttgaaa aagccaagcc
     3961 ccgaatggat cagtatttca accagatgga aaaaatcatt aaagaaaaga agacgtcatc
     4021 ccgcatccgc tttatgctgc aggacgtgct ggatctgcga gggagcaatt gggtgccacg
     4081 ccgaggggat cagggtccca agaccattga ccagatccat aaggaggctg agatggaaga
     4141 acatcgagag cacatcaaag tgcagcagct catggccaag ggcagtgaca agcgtcgggg
     4201 cggtcctcca ggccctccca tcagccgtgg acttcccctt gtggatgatg gtggctggaa
     4261 cacagttccc atcagcaaag gtagccgccc cattgacacc tcacgactca ccaagatcac
     4321 caagcctggc tccatcgatt ctaacaacca gctctttgca cctggagggc gactgagctg
     4381 gggcaagggc agcagcggag gctcaggagc caagccctca gacgcagcat cagaagctgc
     4441 tcgcccagct actagtactt tgaatcgctt ctcagccctt caacaagcgg tacccacaga
     4501 aagcacagat aatagacgtg tggtgcagag gagtagcttg agccgagaac gaggcgagaa
     4561 agctggagac cgaggagacc gcctagagcg gagtgaacgg ggaggggacc gtggggaccg
     4621 gcttgatcgt gcgcggacac ctgctaccaa gcggagcttc agcaaggaag tggaggagcg
     4681 gagtagagaa cggccctccc agcctgaggg gctgcgcaag gcagctagcc tcacggagga
     4741 tcgggaccgt gggcgggatg ccgtgaagcg agaagctgcc ctacccccag tgagccccct
     4801 gaaggcggct ctctctgagg aggagttaga gaagaaatcc aaggctatca ttgaggaata
     4861 tctccatctc aatgacatga aagaggcagt ccagtgcgtg caggagctgg cctcaccctc
     4921 cttgctcttc atctttgtac ggcatggtgt cgagtctacg ctggagcgca gtgccattgc
     4981 tcgtgagcat atggggcagc tgctgcacca gctgctctgt gctgggcatc tgtctactgc
     5041 tcagtactac caagggttgt atgaaatctt ggaattggct gaggacatgg aaattgacat
     5101 cccccacgtg tggctctacc tagcggaact ggtaacaccc attctgcagg aaggtggggt
     5161 gcccatgggg gagctgttca gggagattac aaagcctctg agaccgttgg gcaaagctgc
     5221 ttccctgttg ctggagatcc tgggcctcct gtgcaaaagc atgggtccta aaaaggtggg
     5281 gacgctgtgg cgagaagccg ggcttagctg gaaggaattt ctacctgaag gccaggacat
     5341 tggtgcattc gtcgctgaac agaaggtgga gtataccctg ggagaggagt cggaagcccc
     5401 tggccagagg gcactcccct ccgaggagct gaacaggcag ctggagaagc tgctgaagga
     5461 gggcagcagt aaccagcggg tgttcgactg gatagaggcc aacctgagtg agcagcagat
     5521 agtatccaac acgttagttc gagccctcat gacggctgtc tgctattctg caattatttt
     5581 tgagactccc ctccgagtgg acgttgcagt gctgaaagcg cgagcgaagc tgctgcagaa
     5641 atacctgtgt gacgagcaga aggagctaca ggcgctctac gccctccagg cccttgtagt
     5701 gaccttagaa cagcctccca acctgctgcg gatgttcttt gacgcactgt atgacgagga
     5761 cgtggtgaag gaggatgcct tctacagttg ggagagtagc aaggaccccg ctgagcagca
     5821 gggcaagggt gtggccctta aatctgtcac agccttcttc aagtggctcc gtgaagcaga
     5881 ggaggagtct gaccacaact gatgagaccc agctttcttg tacaaagtgg tctgactcga
     5941 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6001 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6061 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6121 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6181 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     6241 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6301 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6361 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6421 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6481 tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc
     6541 caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     6601 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     6661 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     6721 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     6781 ggatttcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     6841 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     6901 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     6961 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     7021 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     7081 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     7141 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     7201 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     7261 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     7321 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     7381 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     7441 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     7501 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     7561 atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7621 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7681 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7741 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7801 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7861 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7921 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7981 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8041 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8101 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8161 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8221 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8281 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8341 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8401 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8461 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8521 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8581 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8641 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8701 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8761 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8821 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8881 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8941 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9001 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9061 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9121 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9181 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9241 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9301 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9361 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9421 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9481 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9541 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9601 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9661 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9721 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9781 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9841 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9901 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9961 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10021 c
//
