LOCUS Exported 6616 bp ds-DNA circular SYN 23-JUL-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6616) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jul 23, 2020 from SnapGene Viewer 5.1.5 https://www.snapgene.com FEATURES Location/Qualifiers source 1..6616 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 2760..3227 /label=hSyn promoter /note="hSyn promoter" misc_feature 3174..3193 /label=pcheck2_pyc111_F /note="pcheck2_pyc111_F" misc_feature 3254..3259 /label=kozak /note="kozak" misc_feature 3260..3292 /label=GCAMP6f /note="GCAMP6f" misc_feature 3260..3265 /label=T7 tag /note="T7 tag" misc_feature complement(3293..4609) /label=GCAMP6m /note="GCAMP6m" misc_feature complement(3293..3298) /label=NheI /note="NheI" misc_feature complement(4192..4211) /label=pcheck4_pyc111_F /note="pcheck4_pyc111_F" misc_feature 4610..5299 /label=original -L10a /note="original -L10a" misc_feature 4610..4648 /label=orignal GFP-L10 linker /note="orignal GFP-L10 linker" misc_feature 4649..5299 /label=L10 sequence /note=" L10 sequence" misc_feature 5200..5226 /label=ZK168 /note="ZK168" misc_feature complement(5278..5299) /label=ZK108 /note="ZK108" misc_feature 5288..5299 /label=oyc111a_R /note="oyc111a_R" misc_feature 5342..5929 /label=WPRE /note="WPRE" misc_feature complement(5406..5425) /label=R_gcamp_check6 /note="R_gcamp_check6" misc_feature 5958..6444 /label=HGH /note="HGH" ORIGIN 1 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 61 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 121 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc 181 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 241 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 301 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 361 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta 421 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat 481 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 541 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 601 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 661 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 721 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 781 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 841 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 901 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 961 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 1021 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 1081 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 1141 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 1201 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 1261 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 1321 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 1381 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 1441 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 1501 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 1561 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 1621 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 1681 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1741 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 1801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 1861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2581 gccttttgct cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg 2641 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag 2701 tggccaactc catcactagg ggttcctgcg gccgcacgcg tttaattaag tgtctagact 2761 gcagagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg 2821 gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa 2881 ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac 2941 tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc 3001 gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg 3061 gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg 3121 agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc 3181 agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggc 3241 tagaggatcc ggcgccacca tgggttctca tcatcatcat catcatggta tggctagcat 3301 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac 3361 catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg 3421 tcggctgagc tcactcgaga acgtctatat caaggccgac aagcagaaga acggcatcaa 3481 ggcgaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta 3541 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 3601 cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 3661 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg 3721 agggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 3781 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc 3841 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3901 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 3961 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac 4021 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 4081 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 4141 ggggcacaag ctggagtaca acctgccgga ccaactgact gaagagcaga tcgcagaatt 4201 taaagaggct ttctccctat ttgacaagga cggggatggg acaataacaa ccaaggagct 4261 ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat 4321 caatgaagta gatgccgacg gtgacggcac aatcgacttc cctgagttcc tgacaatgat 4381 ggcaagaaaa gggagctaca gggacacgga agaagaaatt agagaagcgt tcggtgtgtt 4441 tgataaggat ggcaatggct acatcagtgc agcagagctt cgccacgtga tgacaaacct 4501 tggagagaag ttaacagatg aagaggttga tgaaatgatc agggaagcag acatcgatgg 4561 ggatggtcag gtaaactacg aagagtttgt acaaatgatg acagcgaagt caggccggac 4621 tcagatctca agctcaagct tcgagttcag cagcaaagtc tcacgcgaca ccctgtacga 4681 ggcggtgcgg gaagtcctgc acgggaacca gcgcaagcgc cgcaagtttc tggagacggt 4741 ggagctgcag atcagcctga agaactacga ccctcagaag gacaaacgtt tctcgggcac 4801 cgtcaggctc aagtccaccc cacgccccaa gttctcggtg tgcgttctgg gggaccagca 4861 gcactgtgat gaagccaagg ccgtggatat cccccacatg gacatcgagg cgctcaagaa 4921 acttaacaaa aacaagaagt tggtcaagaa gctggctaag aagtacgatg cctttttggc 4981 ctctgagtct ctgattaagc agatcccacg tatcctgggc ccaggcctaa acaaggctgg 5041 caagttcccc tccctgctga cacacaatga aaacatggtg gccaaagtgg atgaggtgaa 5101 atcgacaatc aagttccaga tgaagaaggt gctgtgtttg gccgtcgctg ttggccacgt 5161 gaagatgacc gatgatgagc tagtctacaa cattcatctg gctgtcaatt tcttggtgtc 5221 cttgcttaag aaaaactggc aaaacgtgcg ggctctgtac atcaagagca ccatgggcaa 5281 gccccagcgt ctgtattagg gcgcgcccct gcagggaatt cgatatcaag cttatcgata 5341 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 5401 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 5461 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 5521 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5581 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 5641 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 5701 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 5761 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 5821 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 5881 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag 5941 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc 6001 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat 6061 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa 6121 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag 6181 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct 6241 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt 6301 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc 6361 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc 6421 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa 6481 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg 6541 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg 6601 cgcagctgcc tgcagg //