LOCUS       Exported                6616 bp ds-DNA     circular SYN 23-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 23, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2760..3227
                     /label=hSyn promoter
                     /note="hSyn promoter"
     misc_feature    3174..3193
                     /label=pcheck2_pyc111_F
                     /note="pcheck2_pyc111_F"
     misc_feature    3254..3259
                     /label=kozak
                     /note="kozak"
     misc_feature    3260..3292
                     /label=GCAMP6f
                     /note="GCAMP6f"
     misc_feature    3260..3265
                     /label=T7 tag
                     /note="T7 tag"
     misc_feature    complement(3293..4609)
                     /label=GCAMP6m
                     /note="GCAMP6m"
     misc_feature    complement(3293..3298)
                     /label=NheI
                     /note="NheI"
     misc_feature    complement(4192..4211)
                     /label=pcheck4_pyc111_F
                     /note="pcheck4_pyc111_F"
     misc_feature    4610..5299
                     /label=original -L10a
                     /note="original -L10a"
     misc_feature    4610..4648
                     /label=orignal GFP-L10 linker
                     /note="orignal GFP-L10 linker"
     misc_feature    4649..5299
                     /label=L10 sequence
                     /note=" L10 sequence"
     misc_feature    5200..5226
                     /label=ZK168
                     /note="ZK168"
     misc_feature    complement(5278..5299)
                     /label=ZK108
                     /note="ZK108"
     misc_feature    5288..5299
                     /label=oyc111a_R
                     /note="oyc111a_R"
     misc_feature    5342..5929
                     /label=WPRE
                     /note="WPRE"
     misc_feature    complement(5406..5425)
                     /label=R_gcamp_check6
                     /note="R_gcamp_check6"
     misc_feature    5958..6444
                     /label=HGH
                     /note="HGH"
ORIGIN
        1 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
       61 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      121 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
      181 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      241 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
      301 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
      361 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
      421 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
      481 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
      541 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      601 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      661 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
      721 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      781 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      841 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      901 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      961 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1021 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1081 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1141 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1201 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1261 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1321 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1381 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1441 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1501 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1561 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1621 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1681 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1741 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2581 gccttttgct cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
     2641 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
     2701 tggccaactc catcactagg ggttcctgcg gccgcacgcg tttaattaag tgtctagact
     2761 gcagagggcc ctgcgtatga gtgcaagtgg gttttaggac caggatgagg cggggtgggg
     2821 gtgcctacct gacgaccgac cccgacccac tggacaagca cccaaccccc attccccaaa
     2881 ttgcgcatcc cctatcagag agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac
     2941 tgccagcttc agcaccgcgg acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc
     3001 gcctcagcac tgaaggcgcg ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg
     3061 gccaccttgg tcgcgtccgc gccgccgccg gcccagccgg accgcaccac gcgaggcgcg
     3121 agataggggg gcacgggcgc gaccatctgc gctgcggcgc cggcgactca gcgctgcctc
     3181 agtctgcggt gggcagcgga ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggc
     3241 tagaggatcc ggcgccacca tgggttctca tcatcatcat catcatggta tggctagcat
     3301 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac
     3361 catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg
     3421 tcggctgagc tcactcgaga acgtctatat caaggccgac aagcagaaga acggcatcaa
     3481 ggcgaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta
     3541 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3601 cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     3661 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg
     3721 agggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3781 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc
     3841 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3901 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3961 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac
     4021 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4081 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4141 ggggcacaag ctggagtaca acctgccgga ccaactgact gaagagcaga tcgcagaatt
     4201 taaagaggct ttctccctat ttgacaagga cggggatggg acaataacaa ccaaggagct
     4261 ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat
     4321 caatgaagta gatgccgacg gtgacggcac aatcgacttc cctgagttcc tgacaatgat
     4381 ggcaagaaaa gggagctaca gggacacgga agaagaaatt agagaagcgt tcggtgtgtt
     4441 tgataaggat ggcaatggct acatcagtgc agcagagctt cgccacgtga tgacaaacct
     4501 tggagagaag ttaacagatg aagaggttga tgaaatgatc agggaagcag acatcgatgg
     4561 ggatggtcag gtaaactacg aagagtttgt acaaatgatg acagcgaagt caggccggac
     4621 tcagatctca agctcaagct tcgagttcag cagcaaagtc tcacgcgaca ccctgtacga
     4681 ggcggtgcgg gaagtcctgc acgggaacca gcgcaagcgc cgcaagtttc tggagacggt
     4741 ggagctgcag atcagcctga agaactacga ccctcagaag gacaaacgtt tctcgggcac
     4801 cgtcaggctc aagtccaccc cacgccccaa gttctcggtg tgcgttctgg gggaccagca
     4861 gcactgtgat gaagccaagg ccgtggatat cccccacatg gacatcgagg cgctcaagaa
     4921 acttaacaaa aacaagaagt tggtcaagaa gctggctaag aagtacgatg cctttttggc
     4981 ctctgagtct ctgattaagc agatcccacg tatcctgggc ccaggcctaa acaaggctgg
     5041 caagttcccc tccctgctga cacacaatga aaacatggtg gccaaagtgg atgaggtgaa
     5101 atcgacaatc aagttccaga tgaagaaggt gctgtgtttg gccgtcgctg ttggccacgt
     5161 gaagatgacc gatgatgagc tagtctacaa cattcatctg gctgtcaatt tcttggtgtc
     5221 cttgcttaag aaaaactggc aaaacgtgcg ggctctgtac atcaagagca ccatgggcaa
     5281 gccccagcgt ctgtattagg gcgcgcccct gcagggaatt cgatatcaag cttatcgata
     5341 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5401 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5461 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5521 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5581 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5641 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5701 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5761 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5821 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5881 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     5941 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     6001 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     6061 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     6121 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     6181 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     6241 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     6301 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     6361 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     6421 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     6481 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     6541 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     6601 cgcagctgcc tgcagg
//
