LOCUS       Exported                6864 bp ds-DNA     circular SYN 23-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 23, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    590..609
                     /label=pcheck1_pyc111_F
                     /note="pcheck1_pyc111_F"
     misc_feature    735..745
                     /label=5' ITR deletion
                     /note="5' ITR deletion"
     misc_feature    854..1321
                     /label=hSyn promoter
                     /note="hSyn promoter"
     misc_feature    1268..1287
                     /label=pcheck2_pyc111_F
                     /note="pcheck2_pyc111_F"
     misc_feature    1359..1392
                     /label=loxP2722
                     /note="loxP2722"
     misc_feature    1443..1472
                     /label=loxP
                     /note="loxP"
     misc_feature    complement(1465..1496)
                     /label=oyc111a_R
                     /note="oyc111a_R"
     misc_feature    1473..1504
                     /label=oyc111b_R
                     /note="oyc111b_R"
     misc_feature    1473..1496
                     /label=gibsonHR
                     /note="gibsonHR"
     misc_feature    1477..1484
                     /label=AscI
                     /note="AscI"
     misc_feature    complement(1485..2174)
                     /label=original -L10a
                     /note="original -L10a"
     misc_feature    complement(1485..2135)
                     /label=L10 sequence
                     /note=" L10 sequence"
     misc_feature    1485..1506
                     /label=ZK108
                     /note="ZK108"
     misc_feature    complement(1558..1584)
                     /label=ZK168
                     /note="ZK168"
     misc_feature    1913..1932
                     /label=pcheck3_pyc111_F
                     /note="pcheck3_pyc111_F"
     misc_feature    complement(2155..2186)
                     /label=oyc111b_F
                     /note="oyc111b_F"
     misc_feature    2163..2194
                     /label=oyc111c_R
                     /note="oyc111c_R"
     misc_feature    complement(2163..2186)
                     /label=gibsonHR
                     /note="gibsonHR"
     misc_feature    complement(2175..3524)
                     /label=GCAMP6m
                     /note="GCAMP6m"
     misc_feature    2573..2592
                     /label=pcheck4_pyc111_F
                     /note="pcheck4_pyc111_F"
     misc_feature    3310..3329
                     /label=pcheck5_pyc111_F
                     /note="pcheck5_pyc111_F"
     misc_feature    complement(3435..3458)
                     /label=Xpress
                     /note="Xpress"
     misc_feature    complement(3462..3494)
                     /label=T7 tag
                     /note="T7 tag"
     misc_feature    complement(3498..3515)
                     /label=histag
                     /note="histag"
     misc_feature    complement(3505..3536)
                     /label=oyc111c_F
                     /note="oyc111c_F"
     misc_feature    3513..3544
                     /label=oyc111d_F
                     /note="oyc111d_F"
     misc_feature    complement(3513..3536)
                     /label=gibsonHR_gyc111d
                     /note="gibsonHR_gyc111d"
     misc_feature    complement(3525..3530)
                     /label=kozak
                     /note="kozak"
     misc_feature    complement(3531..3536)
                     /label=NheI
                     /note="NheI"
     misc_feature    3537..3570
                     /label=loxP2722
                     /note="loxP2722"
     misc_feature    3621..3654
                     /label=loxP
                     /note="loxP"
     misc_feature    3681..4268
                     /label=WPRE
                     /note="WPRE"
     misc_feature    3983..4002
                     /label=pcheck6_pyc111_F
                     /note="pcheck6_pyc111_F"
     misc_feature    4297..4783
                     /label=HGH
                     /note="HGH"
     misc_feature    complement(6833..6864)
                     /label=oyc111d_R
                     /note="oyc111d_R"
     misc_feature    join(6847..6864,1..15)
                     /label=oyc111a_F
                     /note="oyc111a_F"
     misc_feature    6847..6864
                     /label=gibsonHR_gyc111a
                     /note="gibsonHR_gyc111a"
ORIGIN
        1 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
       61 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      181 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      661 gccttttgct ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact
      721 gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc
      781 gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg cggccgcacg
      841 cgtgtgtcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat
      901 gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac
      961 ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg
     1021 aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg
     1081 cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca
     1141 aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca
     1201 ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga
     1261 ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca
     1321 gtcgagaagg taccggatcc tctagagtcg actccggaat aacttcgtat aggatacttt
     1381 atacgaagtt atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct
     1441 taataacttc gtatagcata cattatacga agttatggcg cgccctaata cagacgctgg
     1501 ggcttgccca tggtgctctt gatgtacaga gcccgcacgt tttgccagtt tttcttaagc
     1561 aaggacacca agaaattgac agccagatga atgttgtaga ctagctcatc atcggtcatc
     1621 ttcacgtggc caacagcgac ggccaaacac agcaccttct tcatctggaa cttgattgtc
     1681 gatttcacct catccacttt ggccaccatg ttttcattgt gtgtcagcag ggaggggaac
     1741 ttgccagcct tgtttaggcc tgggcccagg atacgtggga tctgcttaat cagagactca
     1801 gaggccaaaa aggcatcgta cttcttagcc agcttcttga ccaacttctt gtttttgtta
     1861 agtttcttga gcgcctcgat gtccatgtgg gggatatcca cggccttggc ttcatcacag
     1921 tgctgctggt cccccagaac gcacaccgag aacttggggc gtggggtgga cttgagcctg
     1981 acggtgcccg agaaacgttt gtccttctga gggtcgtagt tcttcaggct gatctgcagc
     2041 tccaccgtct ccagaaactt gcggcgcttg cgctggttcc cgtgcaggac ttcccgcacc
     2101 gcctcgtaca gggtgtcgcg tgagactttg ctgctgaact cgaagcttga gcttgagatc
     2161 tgagtccggc ctgacttcgc tgtcatcatt tgtacaaact cttcgtagtt tacctgacca
     2221 tccccatcga tgtctgcttc cctgatcatt tcatcaacct cttcatctgt taacttctct
     2281 ccaaggtttg tcatcacgtg gcgaagctct gctgcactga tgtagccatt gccatcctta
     2341 tcaaacacac cgaacgcttc tctaatttct tcttccgtgt ccctgtagct cccttttctt
     2401 gccatcattg tcaggaactc agggaagtcg attgtgccgt caccgtcggc atctacttca
     2461 ttgatcatgt cctgcagctc tgcttctgtg gggttctgcc ccagagaccg catcaccgtc
     2521 cccagctcct tggttgttat tgtcccatcc ccgtccttgt caaataggga gaaagcctct
     2581 ttaaattctg cgatctgctc ttcagtcagt tggtccggca ggttgtactc cagcttgtgc
     2641 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
     2701 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
     2761 atggtgcgct cctggatgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
     2821 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg
     2881 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
     2941 gtggcatcgc cctcaccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
     3001 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatgctc
     3061 cctccggtac cgcccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     3121 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttcgaaag tttggactgc
     3181 acgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     3241 tggtagtggt aggcgagctg cacgccgccg tcctcgatgt tgtggcggat cttgaagttc
     3301 gccttgatgc cgttcttctg cttgtcggcc ttgatataga cgttctcgag tgagctcagc
     3361 cgacctatag ctctgactgc gtgacctgtc ttattccact tacgacgtga tgagtcgacc
     3421 atggtggcga gatccttatc gtcatcgtcg tacagatccc gacccatttg ctgtccacca
     3481 gtcatgctag ccataccatg atgatgatga tgatgagaac ccatggtggc gctagcataa
     3541 cttcgtataa agtatcctat acgaagttat ttgccttaac ccagaaatta tcactgttat
     3601 tctttagaat ggtgcaaaga ataacttcgt ataatgtatg ctatacgaag ttatgaattc
     3661 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3721 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3781 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3841 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3901 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3961 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4021 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4081 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     4141 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4201 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4261 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     4321 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     4381 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     4441 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     4501 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     4561 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     4621 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     4681 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     4741 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     4801 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4861 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4921 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     4981 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     5041 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5101 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5161 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5221 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     5281 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5341 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     5401 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5461 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     5521 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5581 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5641 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     5701 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5761 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5821 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5881 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5941 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6001 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6061 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6121 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6181 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6241 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6301 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6361 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6421 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6481 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6541 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6601 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6661 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6721 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6781 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6841 ccttaacgtg agttttcgtt ccac
//
