LOCUS       ptag_168_GCaMP6m        8143 bp    DNA        circular     22-JUN-2020
DEFINITION  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    54..2717
                     /locus_tag="ptag-168"
                     /label="ptag-168"
                     /ApEinfo_label="ptag-168"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6325..6984
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    7142..8143
                     /locus_tag="pBR322_Coli origin"
                     /label="pBR322_Coli origin"
                     /ApEinfo_label="pBR322_Coli origin"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2848..4884
                     /locus_tag="Insert"
                     /label="Insert"
                     /ApEinfo_label="Insert"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4898..5654
                     /locus_tag="unc-54 3'-UTR"
                     /label="unc-54 3'-UTR"
                     /ApEinfo_label="unc-54 3'-UTR"
                     /ApEinfo_fwdcolor="#7ea7d8"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2848..4197
                     /locus_tag="GCaMP6m"
                     /label="GCaMP6m"
                     /ApEinfo_label="GCaMP6m"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2763..2804
                     /locus_tag="Artificial intron"
                     /label="Artificial intron"
                     /ApEinfo_label="Artificial intron"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4237..4884
                     /locus_tag="rpl-1a"
                     /label="rpl-1a"
                     /ApEinfo_label="rpl-1a"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4198..4236
                     /locus_tag="Linker"
                     /label="Linker"
                     /ApEinfo_label="Linker"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ATGACCATGA TTACGCCAAG CTTGCATGCG CGGCCGCACT GACTGGGCCG GCCggatcca
       61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
      121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
      181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
      241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
      301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
      361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
      421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
      481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
      541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
      601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
      661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
      721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
      781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
      841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
      901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
      961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
     1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
     1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
     1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
     1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
     1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
     1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttta atttcgaatt
     1381 tttttaaact tgttgtaact tttttccctg tcggcgccaa ttaacaaaat acgtagaatt
     1441 ttgcgcttat tgttttgtta gttaacaaat ttttgacaac tttcttatca gctgagattt
     1501 aggtgtttca gttcgcaatc tgcgtctctc cttgctctcc tctattttaa aacccccata
     1561 actttgttct tgtgagagtt atcaaaaaat tgtcaactaa caaaatatag agcacaaaaa
     1621 tatgcacatt ttgtatgttg acaatttttt gatacctttt ttagttttcg agatatctta
     1681 gttttagttt agctgtacct ttttctggca aaaaacttcc aaatttcggc aatttttcgg
     1741 caaatttccc ggcaaatttc gccaagtaca tcgggctctc agacaagtgg tctgtgagtg
     1801 gaagtcgata aaagtagttt acgtgctctc cgtccccccg cttcttctcc gtctctgctt
     1861 ctatttctcc attgactagt cccactactt tttacggggc ggagggctct cttgcactcc
     1921 agcttcttct ctttttgtgc tcttttttgc ccgtatgtgg gtgctcattt atttttcttt
     1981 ggttttaaaa taaattcggt ttattaggct actcgtaaaa atgaagaaaa aacttcaaat
     2041 tcaaacgaca ttaaaaaaaa atttaataaa aattttttga attgctcaaa agccccacct
     2101 atatatatat atagctccgc ctacttctcg cacagacccc gcccactttt tgactccgcc
     2161 cccttattgg caaaagttct tgaaaaaaat aaaaataaaa attcaaattg aaacaatttt
     2221 tttttaatga agtagttttt ttctaaattg atgaaaattt ctaaacagtt tacaagcacc
     2281 gcccactttt tgactccgcc cctttgttgg aaaaattctt gtaaaaaaaa ataaaaaata
     2341 aaagcttctc tttcaaacga tttttagttg gtttttgtat gaaaagtttt tttcctaaaa
     2401 tagaaatgtc tgaataattt gcaagctccg cccttttctt acccagactc cgccccttta
     2461 ttgactaatt tttttttgtc catttacctg aagttcaaaa aaaaaattta cagtttcata
     2521 gagtaataat ttttcaaaaa tctctttttc caatgtttaa tgtgtcatta taaacccgcc
     2581 cagttttaag ctccgcccat ttaggccccg cccatttatg acccaaatat ttttttctct
     2641 attcacccgt ggatttccct ccaacaagtt accccaaaaa taaaaataaa aattcctgca
     2701 ggcgcccacc gggatccGGC GCGCCTCTAG AGGATCCCCG GGATTGGCCA AAGGACCCAA
     2761 AGGTATGTTT CGAATGATAC TAACATAACA TAGAACATTT TCAGGAGGAC CCTTGGCTAG
     2821 CGTCGACGGT ACCATGGTAT TGATATCATG GGTTCTCATC ATCATCATCA TCATGGTATG
     2881 GCTAGCATGA CTGGTGGACA GCAAATGGGT CGGGATCTGT ACGACGATGA CGATAAGGAT
     2941 CTCGCCACCA TGGTCGACTC ATCACGTCGT AAGTGGAATA AGACAGGTCA CGCAGTCAGA
     3001 GCTATAGGTC GGCTGAGCTC ACTCGAGAAC GTCTATATCA AGGCCGACAA GCAGAAGAAC
     3061 GGCATCAAGG CGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC
     3121 TACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
     3181 TACCTGAGCG TGCAGTCCAA ACTTTCGAAA GACCCCAACG AGAAGCGCGA TCACATGGTC
     3241 CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGC
     3301 GGTACCGGAG GGAGCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
     3361 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGTGAG
     3421 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
     3481 GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC
     3541 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACATCCAG
     3601 GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC
     3661 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
     3721 AACATCCTGG GGCACAAGCT GGAGTACAAC CTGCCGGACC AACTGACTGA AGAGCAGATC
     3781 GCAGAATTTA AAGAGGCTTT CTCCCTATTT GACAAGGACG GGGATGGGAC AATAACAACC
     3841 AAGGAGCTGG GGACGGTGAT GCGGTCTCTG GGGCAGAACC CCACAGAAGC AGAGCTGCAG
     3901 GACATGATCA ATGAAGTAGA TGCCGACGGT GACGGCACAA TCGACTTCCC TGAGTTCCTG
     3961 ACAATGATGG CAAGAAAAGG GAGCTACAGG GACACGGAAG AAGAAATTAG AGAAGCGTTC
     4021 GGTGTGTTTG ATAAGGATGG CAATGGCTAC ATCAGTGCAG CAGAGCTTCG CCACGTGATG
     4081 ACAAACCTTG GAGAGAAGTT AACAGATGAA GAGGTTGATG AAATGATCAG GGAAGCAGAC
     4141 ATCGATGGGG ATGGTCAGGT AAACTACGAA GAGTTTGTAC AAATGATGAC AGCGAAGTCa
     4201 GGCCGGACTC AGATCTCaAG CTCAAGCTTC GAgTTCtcga aggtttcccg cgagtctctt
     4261 aatgaggcta tcgctgaggt ccttaaggga tcctcggaaa agccacgcaa gttccgtgag
     4321 actatcgagc tccagatcgg tctcaagaac tacgacccac agaaggacaa gcgtttcagc
     4381 ggatccatca gactgaagca cattccacgc ccaaacatga aggtgtgcgt cttcggagac
     4441 cagcaccatc ttgacgaggc tgccgccgga gacattccat cgatgagcgc cgatgacttg
     4501 aagaagctta acaagcagaa gaagctcatc aagaagctcg ccaagagcta cgatgctttc
     4561 atcgcttccg aatccttgat caagcagatc ccacgtatcc tcggtccagg actcaacaag
     4621 gccggaaaat tcccttccgt cgttacccac ggagaatctc tccaaagcaa gagtgacgag
     4681 atccgcgcca ccgtcaagtt ccagatgaag aaggttctct gcctctccgt cgccgtcggt
     4741 cacgttggac tcacccagga ggagcttgtc tccaacatct ccctctccat caacttcctc
     4801 gtctcgctct tgaagaagaa ctggcagaac gtgagatcgt tgaacatcaa gtccaccatg
     4861 ggaaagccac aaagagttta ttaagatATC TGAGCTCCGC ATCGGCCGCT GTCATCAGAT
     4921 CGCCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT
     4981 GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT ATCAAAAAAA CTTCTTCTTA
     5041 ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA
     5101 AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA
     5161 ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT
     5221 GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA
     5281 CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA
     5341 AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG
     5401 TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT
     5461 TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG
     5521 ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA
     5581 AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA
     5641 AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA
     5701 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC
     5761 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG
     5821 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA
     5881 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAT TAAGGGCCTC
     5941 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT
     6001 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA
     6061 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG
     6121 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC
     6181 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG
     6241 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT
     6301 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA
     6361 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT
     6421 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA
     6481 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA
     6541 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT
     6601 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC
     6661 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT
     6721 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT
     6781 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT
     6841 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT
     6901 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA
     6961 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG
     7021 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT
     7081 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA
     7141 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA
     7201 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT
     7261 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG
     7321 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
     7381 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA
     7441 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC
     7501 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC
     7561 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA
     7621 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG
     7681 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA
     7741 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT
     7801 CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG
     7861 TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA
     7921 GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC
     7981 AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG
     8041 AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG
     8101 TGTGGAATTG TGagcggata acaatttcac acaggAAACA GCT 
//
