LOCUS       pAS19_pSM_psra-6_GCaMP6 9272 bp ds-DNA     circular     18-SEP-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3892..3933
                     /label="Artificial intron"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3977..5326
                     /label="GCaMP6m"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    6027..6783
                     /label="unc-54 3'-UTR"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    7454..8113
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3977..6013
                     /label="Insert"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5366..6013
                     /label="rpl-1a"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    54..3846
                     /label="psra-6"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    8271..9272
                     /label="pBR322_Coli origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5327..5365
                     /label="Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ATGACCATGA TTACGCCAAG CTTGCATGCG CGGCCGCACT GACTGGGCCG GCCCCTTCAG
       61 TGCCTACGTG CCAGCATTTT TTGTCGTAGA GGCATGTGCA GGTTGCCTTA AAAGCACGCA
      121 AACATTCTTC GCCTACCTGG CAGTTCTCAT CCAGATTATT CTGTCAAAGA AGTTGCTTTA
      181 TTTTTACGTG AACCTAAATA CACATTAATT GATACAGTGT TCCACCAAAA TTTATAAATT
      241 TATTCTTCTT TCTCTCAAGA AATCGTCATG CGTTATCTTT CCAATTTGCT GTGCCTAACT
      301 CTGACGTTTA TCGTTTGCTT TGGCGCCGAG CGCGATGCAG ATGAAGAAGC TTATGTACTG
      361 ATGAATCTTC TGAAACAATC TATTGATATG GACCAAACTG AAGCTCTTCC AGGATTTGTA
      421 TCGAGGGATT TCACATATGA TACTTGCAAG GGAATTTGGA ATAGAGGTAT ACAACTGATT
      481 ATTAGATTTT TTCAAAATGA AATCAATTAC AGATAAATAT ATGGAGTATC TGAAAGTCTT
      541 TTCATCTCGA CCAGACGGTG GTATGCAACC AATGTCCACT GATGTACCTT TCTATCTTTC
      601 TAAACTTTGG CATTTGTATT TTAATTTCAG TGGAAGGTTA CAAATGCACA TTTCAACGAC
      661 AATACTCTTT ATTTCAATAT TCACAACTAC AATCAACACT TAGCAATCTA TCCTGATTTG
      721 GAATTTGAAG CTAAAAAAGT GAGCATGAAG AAGGTAGAGG TTTTCTAAAC TTGGTTTTTC
      781 AGATCGGTAA AGACGAACGT TACATTTTGA CAAGAGGAAA AATTAGCAAA TGTGGGACGT
      841 TGGAAAAAGA ATATTAACTT TTAGATATAA TAAATCGAAA TTGAAATGTA TCTTTTTATG
      901 CAATGTACAA TAGAAATCAT ATGAAGTCTA AAACATGTTA GATAGTATGC TGCACTATAA
      961 GGATTGAATC TAAGTGTAAG AAATACTCAT TATTTTTAGA CTTTTGAGAA CAATAATAGT
     1021 GGTACTGTTT GAACTGTGAC CAAACTTCAG CAAATTGAAG TTGTTTAAAG CATGAAGCTG
     1081 TTCAAATCCA CGAAGCTTTG CTCTCCGGTC TTATTTCCAA GTATATTTTA TAATTTGGCA
     1141 GATCATCTTT TTCTCAGCCA AGTGTTGAAA TCATCGTGTT CAGAAGGCAT GTGCAATCCC
     1201 AATCTGATTC GAGCATTGAC GACCTTAAAT TTGGCCTTTG GTATTTTGAA ACGCATTTAA
     1261 TTGATTCAAG CTTACTTTAG AGTTGTTATC TTACCTCAAT GTAGTTAGTG TTGTAGAACA
     1321 TATTTCTATG AGTGCCCCAT AAACCAGAAA AGTCAAACGA AAATGTTTCA GATATCAAAA
     1381 AAAGTATCAC AAAGCATGCG AAAAAACCAA ATCTGAGGTG CATTTGCGAG GGGCACTTCA
     1441 GATTTGAAAC TTTGAATTTT GCACCTCGCC ATGTCTATAA AAGGAGGAGT TTGATAATAT
     1501 CTGACGCAAG TAGAGTACAA TTTTATTAAT CCAAATAAAT TGTGCCAATT TTTATAAGAT
     1561 GATATAAATG AGAAACGGGA ATTGAGAATA TGATGAGTAG TTTTTAAAGT TTGTCATTCA
     1621 AAATTGTTGT TGCCTGCCAT GCAAATGAAA GTCAGAAGAG TTCCAAACCA GAAGAGGAAA
     1681 TATGATTTTT CCATGTAACA GTAGTTGTCT TTTTTTAGAA CGGCAGGCCA AACACAAAAG
     1741 TATTCCATTA TTTCCTGATT TGCCTTTTCT AAACAATTTC CAGTCAAGTG ATAAATTCAC
     1801 ATTTCCAGTG CTCTGAAAAT CTTGTCAAAA AGTTTGTATT TCGGATGTCA GGTGATGTTT
     1861 GTAAGACATA CGTTAGCTGG TCTCTTACGT ATTCCAAAAC CGCTACAATA TGATAATCAG
     1921 TCAAAATGAA ACACTAAAGT TTTTGAACTG AATGATCACA GAGTTGATTG AGATAGATAG
     1981 AGTTATAATT TTGAAGGAAT CTTACGCATA CGGAAGCTTC AAAGAGTTTA GGATAATTTC
     2041 ATAGTTAGAG TAGACGTGAC AAAGTTATTA ATATCATAAT TTGTTTTTCT CGAAATTGAA
     2101 TATGACTGAA ACGCAAGTCA GAGAGATTCC TGAACCTAAT AATCTAAAAT TTTAATTGCT
     2161 ATTTTCAATA TTGCATTTTT CGATTCACGC GTAAAGGAAG AGACAATTTC ACAGCATCGG
     2221 AACTATCTTC CGATTTGGAA GATAATTTTT TAAAGTGTTT TCCTTATTGG CTTTGAAATT
     2281 TCAATAAAAG TACACCTGAC GAATTTTCAA ATAAGTGAAA AATAGAAGCA GACATCGAGT
     2341 AAACGTGCAT AAAATAAATG CAATAAGTGC TCGTTAAGTA ATGAAATAAA GTTAGTCTAC
     2401 AAATTGATCA ACCAGTACAT GACTAAAAAT TGGTGACTGT TTTGTTTTAG TCTTTACCCA
     2461 ATTTTTATTT CAGCGATTTC ATTTTATACT CAAATGTGAT TTTTCTACAT TTTTTCGAAT
     2521 TTAGAAAAGA TCAGACGATT TCCTTAAAGA GGATTTCTAG TTTAGTCTTT GATTCAATAA
     2581 CTTATAGTAC AGCTTAGTTA TAACTATAGT TCACTATGAA ATATCACATT TATAATCGTT
     2641 TCATTTAATT GTAAGGACAT TTTTGCATCA AACGCCAGTT TTCTGTCCCC ATTTAATTAT
     2701 AAGTTTTACT CGGCCGATGC GAGATGGATG CTCCTCTCAC ACACCTTACC CCACGCCTTG
     2761 ACACCTTATT TATTGGGTTT TAAATTTTTT TGATATTTTA GAGAATTATA AAGGTTTCCG
     2821 TTAAAAAAAA ACATGGCCTT CAAGGCGAGT ACGTCTGCGT TGTAAACTCA CAAATAAACT
     2881 TAAAAAATAT TTTTTTTTGG ATTGAAGCTT TATTTGAAAG GATAACTCAC TCGCTATCTA
     2941 AAAATTGAGA AAGTTTTGTA ATAACACGAA ATTTCATGAA GTGCGGAAAA TTTTTGAAAA
     3001 TTGGATGAAA CTGTTCCTTT CACGAGTTTG GTCGTGTTTT ATTCTTCATT CAAATACCAA
     3061 TGCAACAGTC GGTTTTAACT TTTCAAAATT ATACTTCAAG TTTTTACAAG TGCATATGTA
     3121 AAAGTGTTTT CTATCTTCAC ACCTTCGAAA ACAGCAATAA TAAATTAAAG CCGCGTGCAG
     3181 CTATCTTATA GTTTGGTTCT CTGTATTTTC AAATATCTTA AGTGTCACAT CAAATTCAAT
     3241 GATAATTTGG AAATCACAGA AGATCTTTAA TGTGTTTATA GTCTCTATGA ACTCATCAAT
     3301 TACAAATATA TTTCTAGTCA GACTATTCAC ACAAACCGCA GTTTTTAATC ATCCATATCT
     3361 TCTTCTCATG GAAGTTCTAG CTAACGTCAA GTCAGACTTT CACAAGTATG AGTTTTATTT
     3421 TGTCCAACAT TTTATGCTCT TGATGAACTC AAATATCATT TCTCTTTAAA CTATAGTTTC
     3481 ATAAGATTCA GTAATTTTGA TTCTGCAGTT CAGATTCTAA ATTTATCCGA AGAGAAAACC
     3541 AATTGATCAA GTCTTTATTC AATTTCCGAG AAAACGTGGT TGAGCTTAAC ACATTTTAGA
     3601 ATTTGTAAAC GTGTTGTTGT TTTTCAAAAC CTCGCTAATA ATTTCTCCCT AGTTACTCCC
     3661 GTAAAGTATT TTCTGGAAAA TTTGCAGGTT TAATAGAGTT GGTTTGAAAT ACTGCAAATC
     3721 AAAACACTTT TTATAGATTA AATCAATTTT GATAATGTTT TCTGTCACAC ATAATAAACT
     3781 GCACTCGCAG AATTTAATAT TTATTATTTC AGATTTTGCC ATTTCTAATT TAAGTTGTGC
     3841 TGCTCCGGCG CGCCTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA GGTATGTTTC
     3901 GAATGATACT AACATAACAT AGAACATTTT CAGGAGGACC CTTGGCTAGC GTCGACGGTA
     3961 CCATGGTATT GATATCATGG GTTCTCATCA TCATCATCAT CATGGTATGG CTAGCATGAC
     4021 TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC TCGCCACCAT
     4081 GGTCGACTCA TCACGTCGTA AGTGGAATAA GACAGGTCAC GCAGTCAGAG CTATAGGTCG
     4141 GCTGAGCTCA CTCGAGAACG TCTATATCAA GGCCGACAAG CAGAAGAACG GCATCAAGGC
     4201 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCT ACCACTACCA
     4261 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCGT
     4321 GCAGTCCAAA CTTTCGAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
     4381 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGCG GTACCGGAGG
     4441 GAGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
     4501 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGTGAGG GCGATGCCAC
     4561 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
     4621 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT
     4681 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACATCCAGG AGCGCACCAT
     4741 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
     4801 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
     4861 GCACAAGCTG GAGTACAACC TGCCGGACCA ACTGACTGAA GAGCAGATCG CAGAATTTAA
     4921 AGAGGCTTTC TCCCTATTTG ACAAGGACGG GGATGGGACA ATAACAACCA AGGAGCTGGG
     4981 GACGGTGATG CGGTCTCTGG GGCAGAACCC CACAGAAGCA GAGCTGCAGG ACATGATCAA
     5041 TGAAGTAGAT GCCGACGGTG ACGGCACAAT CGACTTCCCT GAGTTCCTGA CAATGATGGC
     5101 AAGAAAAGGG AGCTACAGGG ACACGGAAGA AGAAATTAGA GAAGCGTTCG GTGTGTTTGA
     5161 TAAGGATGGC AATGGCTACA TCAGTGCAGC AGAGCTTCGC CACGTGATGA CAAACCTTGG
     5221 AGAGAAGTTA ACAGATGAAG AGGTTGATGA AATGATCAGG GAAGCAGACA TCGATGGGGA
     5281 TGGTCAGGTA AACTACGAAG AGTTTGTACA AATGATGACA GCGAAGTCaG GCCGGACTCA
     5341 GATCTCaAGC TCAAGCTTCG AgTTCtcgaa ggtttcccgc gagtctctta atgaggctat
     5401 cgctgaggtc cttaagggat cctcggaaaa gccacgcaag ttccgtgaga ctatcgagct
     5461 ccagatcggt ctcaagaact acgacccaca gaaggacaag cgtttcagcg gatccatcag
     5521 actgaagcac attccacgcc caaacatgaa ggtgtgcgtc ttcggagacc agcaccatct
     5581 tgacgaggct gccgccggag acattccatc gatgagcgcc gatgacttga agaagcttaa
     5641 caagcagaag aagctcatca agaagctcgc caagagctac gatgctttca tcgcttccga
     5701 atccttgatc aagcagatcc cacgtatcct cggtccagga ctcaacaagg ccggaaaatt
     5761 cccttccgtc gttacccacg gagaatctct ccaaagcaag agtgacgaga tccgcgccac
     5821 cgtcaagttc cagatgaaga aggttctctg cctctccgtc gccgtcggtc acgttggact
     5881 cacccaggag gagcttgtct ccaacatctc cctctccatc aacttcctcg tctcgctctt
     5941 gaagaagaac tggcagaacg tgagatcgtt gaacatcaag tccaccatgg gaaagccaca
     6001 aagagtttat taagatATCT GAGCTCCGCA TCGGCCGCTG TCATCAGATC GCCATCTCGC
     6061 GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC
     6121 CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAAAC TTCTTCTTAA TTTCTTTGTT
     6181 TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA
     6241 ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC
     6301 CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT
     6361 TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT
     6421 ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA
     6481 TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT
     6541 AATTTTCCTG CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG
     6601 CGTTTTTCTT GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG
     6661 GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT
     6721 GTCTATGGGG TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT
     6781 TTCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA
     6841 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA
     6901 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT
     6961 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA
     7021 GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAATT AAGGGCCTCG TGATACGCCT
     7081 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG
     7141 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC
     7201 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG
     7261 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT
     7321 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT
     7381 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA
     7441 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT
     7501 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA
     7561 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG
     7621 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG
     7681 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG
     7741 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT
     7801 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG
     7861 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC
     7921 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG
     7981 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC
     8041 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT
     8101 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA
     8161 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA
     8221 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
     8281 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
     8341 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
     8401 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA
     8461 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
     8521 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
     8581 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
     8641 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC
     8701 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
     8761 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
     8821 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
     8881 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT
     8941 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC
     9001 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG
     9061 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA
     9121 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC
     9181 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT
     9241 Gagcggataa caatttcaca caggAAACAG CT
//