LOCUS       Exported               10685 bp ds-DNA     circular SYN 23-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    E3.pcDNA5 tetO BirA Fg N-term eIF4G1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 23, 2020 from SnapGene Viewer 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    234..680
                     /locus_tag="rTTA tetO CMV2"
                     /label=rTTA tetO CMV2
                     /note="rTTA tetO CMV2"
     misc_feature    718..1680
                     /locus_tag="BirA"
                     /label=BirA
                     /note="BirA"
     misc_feature    1711..1734
                     /locus_tag="Flag tag"
                     /label=Flag tag
                     /note="Flag tag"
     misc_feature    1748..1765
                     /locus_tag="attR1"
                     /label=attR1
                     /note="attR1"
     misc_feature    1774..6573
                     /locus_tag="EIF4G1"
                     /label=EIF4G1
                     /note="EIF4G1"
     misc_feature    6574..6594
                     /locus_tag="attR2"
                     /label=attR2
                     /note="attR2"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtctcgagg
      241 gatccaagct ggccgcacgt ctccctatca gtgatagaga agtcgacacg tctcgagctc
      301 cctatcagtg atagagaagg tacgtctaga acgtctccct atcagtgata gagaagtcga
      361 cacgtctcga gctccctatc agtgatagag aaggtacgtc tagaacgtct ccctatcagt
      421 gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta cgtctagaac
      481 gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag tgatagagaa
      541 ggtaccccct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      601 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggaggctgg
      661 atcctctagc gtttaaactt aagcttggta ccgagctcgg atccagctag cgccaccatg
      721 aaggacaaca ccgtgcccct gaagctgatc gccctgctgg ccaacggcga gttccacagc
      781 ggcgagcagc tgggcgagac cctgggcatg agccgggccg ccatcaacaa gcacatccag
      841 accctgcggg actggggcgt ggacgtgttc accgtgcccg gcaagggcta cagcctgccc
      901 gagcccatcc agctgctgaa cgccaagcag atcctgggcc agctggacgg cggcagcgtg
      961 gccgtgctgc ccgtgatcga cagcaccaac cagtacctgc tggaccggat cggcgagctg
     1021 aagagcggcg acgcctgcat cgccgagtac cagcaggccg gccggggcgg ccggggccgg
     1081 aagtggttca gccccttcgg cgccaacctg tacctgagca tgttctggcg gctggagcag
     1141 ggccccgccg ccgccatcgg cctgagcctg gtgatcggca tcgtgatggc cgaggtgctg
     1201 cggaagctgg gcgccgacaa ggtgcgggtg aagtggccca acgacctgta cctgcaggac
     1261 cggaagctgg ccggcatcct ggtggagctg accggcaaga ccggcgacgc cgcccagatc
     1321 gtgatcggcg ccggcatcaa catggccatg cggcgggtgg aggagagcgt ggtgaaccag
     1381 ggctggatca ccctgcagga ggccggcatc aacctggacc ggaacaccct ggccgccatg
     1441 ctgatccggg agctgcgggc cgccctggag ctgttcgagc aggagggcct ggccccctac
     1501 ctgagccggt gggagaagct ggacaacttc atcaaccggc ccgtgaagct gatcatcggc
     1561 gacaaggaga tcttcggcat cagccggggc atcgacaagc agggcgccct gctgctggag
     1621 caggacggca tcatcaagcc ctggatgggc ggcgagatca gcctgcggag cgccgagaag
     1681 gggggatctg gccccggcgg cggcgcgcca gactacaaag acgatgacga taaactaggc
     1741 gcgcccacaa gtttgtacaa aaaagcaggc ttcaacaaag ctccacagtc cacaggcccc
     1801 ccacccgccc catcccccgg actcccacag ccagcgtttc ccccggggca gacagcgccg
     1861 gtggtgttca gtacgccaca agcgacacaa atgaacacgc cttctcagcc ccgccagcac
     1921 ttctacccta gccgggccca gcccccgagc agtgcagcct cccgagtgca gagtgcagcc
     1981 cctgcccgcc ctggcccagc tgcccatgtc taccctgctg gatcccaagt aatgatgatc
     2041 ccttcccaga tctcctaccc agcctcccag ggggcctact acatccctgg acaggggcgt
     2101 tccacatacg ttgtcccgac acagcagtac cctgtgcagc caggagcccc aggcttctat
     2161 ccaggtgcaa gccctacaga atttgggacc tacgctggcg cctactatcc agcccaaggg
     2221 gtgcagcagt ttcccactgg cgtggccccc gccccagttt tgatgaacca gccaccccag
     2281 attgctccca agagggagcg taagacgatc cgaattcgag atccaaacca aggaggaaag
     2341 gatatcacag aggagatcat gtctggggcc cgcactgcct ccacacccac ccctccccag
     2401 acgggaggcg gtctggagcc tcaagctaat ggggagacgc cccaggttgc tgtcattgtc
     2461 cggccagatg accggtcaca gggagcaatc attgctgacc ggccagggct gcctggccca
     2521 gagcatagcc cttcagaatc ccagccttcg tcgccttctc cgaccccatc accatcccca
     2581 gtcttggaac cggggtctga gcctaatctc gcagtcctct ctattcctgg ggacactatg
     2641 acaactatac aaatgtctgt agaagaatca acccccatct cccgtgaaac tggggagcca
     2701 tatcgcctct ctccagaacc cactcctctc gccgaaccca tactggaagt agaagtgaca
     2761 cttagcaaac cggttccaga atctgagttt tcttccagtc ctctccaggc tcccacccct
     2821 ttggcatctc acacagtgga aattcatgag cctaatggca tggtcccatc tgaagatctg
     2881 gaaccagagg tggagtcaag cccagagctt gctcctcccc cagcttgccc ctccgaatcc
     2941 cctgtgccca ttgctccaac tgcccaacct gaggaactgc tcaacggagc cccctcgcca
     3001 ccagctgtgg acttaagccc agtcagtgag ccagaggagc aggccaagga ggtgacagca
     3061 tcagtggcgc cccccaccat cccctctgct actccagcta cggctccttc agctacttcc
     3121 ccagctcagg aggaggaaat ggaagaagaa gaagaagagg aagaaggaga agcaggagaa
     3181 gcaggagaag ctgagagtga gaaaggagga gaggaactgc tccccccaga gagtacccct
     3241 attccagcca acttgtctca gaatttggag gcagcagcag ccactcaagt ggcagtatct
     3301 gtgccaaaga ggagacggaa aattaaggag ctaaataaga aggaggctgt tggagacctt
     3361 ctggatgcct tcaaggaggc gaacccggca gtaccagagg tggaaaatca gcctcctgca
     3421 ggcagcaatc caggcccaga gtctgagggc agtggtgtgc ccccacgtcc tgaggaagca
     3481 gatgagacct gggactcaaa ggaagacaaa attcacaatg ctgagaacat ccagcccggg
     3541 gaacagaagt atgaatataa gtcagatcag tggaagcctc taaacctaga ggagaaaaaa
     3601 cgttacgacc gtgagttcct gcttggtttt cagttcatct ttgccagtat gcagaagcca
     3661 gagggattgc cacatatcag tgacgtggtg ctggacaagg ccaataaaac accactgcgg
     3721 ccactggatc ccactagact acaaggcata aattgtggcc cagacttcac tccatccttt
     3781 gccaaccttg gccggacaac ccttagcacc cgtgggcccc caaggggtgg gccaggtggg
     3841 gagctgcccc gtgggccggc tggcctggga ccccggcgct ctcagcaggg accccgaaaa
     3901 gaaccacgca agatcattgc cacagtgtta atgaccgaag atataaaact gaacaaagca
     3961 gagaaagcct ggaaacccag cagcaagcgg acggcggctg ataaggatcg aggggaagaa
     4021 gatgctgatg gcagcaaaac ccaggaccta ttccgcaggg tgcgctccat cctgaataaa
     4081 ctgacacccc agatgttcca gcagctgatg aagcaagtga cgcagctggc catcgacacc
     4141 gaggaacgcc tcaaaggggt cattgacctc atttttgaga aggccatttc agagcccaac
     4201 ttctctgtgg cctatgccaa catgtgccgc tgcctcatgg cgctgaaagt gcccactacg
     4261 gaaaagccaa cagtgactgt gaacttccga aagctgttgt tgaatcgatg tcagaaggag
     4321 tttgagaaag acaaagatga tgatgaggtt tttgagaaga agcaaaaaga gatggatgaa
     4381 gctgctacgg cagaggaacg aggacgcctg aaggaagagc tggaagaggc tcgggacata
     4441 gcccggcggc gctctttagg gaatatcaag tttattggag agttgttcaa actgaagatg
     4501 ttaacagagg caataatgca tgactgtgtg gtcaaactgc ttaagaacca tgatgaagag
     4561 tcccttgagt gcctttgtcg tctgctcacc accattggca aagacctgga ctttgaaaaa
     4621 gccaagcccc gaatggatca gtatttcaac cagatggaaa aaatcattaa agaaaagaag
     4681 acgtcatccc gcatccgctt tatgctgcag gacgtgctgg atctgcgagg gagcaattgg
     4741 gtgccacgcc gaggggatca gggtcccaag accattgacc agatccataa ggaggctgag
     4801 atggaagaac atcgagagca catcaaagtg cagcagctca tggccaaggg cagtgacaag
     4861 cgtcggggcg gtcctccagg ccctcccatc agccgtggac ttccccttgt ggatgatggt
     4921 ggctggaaca cagttcccat cagcaaaggt agccgcccca ttgacacctc acgactcacc
     4981 aagatcacca agcctggctc catcgattct aacaaccagc tctttgcacc tggagggcga
     5041 ctgagctggg gcaagggcag cagcggaggc tcaggagcca agccctcaga cgcagcatca
     5101 gaagctgctc gcccagctac tagtactttg aatcgcttct cagcccttca acaagcggta
     5161 cccacagaaa gcacagataa tagacgtgtg gtgcagagga gtagcttgag ccgagaacga
     5221 ggcgagaaag ctggagaccg aggagaccgc ctagagcgga gtgaacgggg aggggaccgt
     5281 ggggaccggc ttgatcgtgc gcggacacct gctaccaagc ggagcttcag caaggaagtg
     5341 gaggagcgga gtagagaacg gccctcccag cctgaggggc tgcgcaaggc agctagcctc
     5401 acggaggatc gggaccgtgg gcgggatgcc gtgaagcgag aagctgccct acccccagtg
     5461 agccccctga aggcggctct ctctgaggag gagttagaga agaaatccaa ggctatcatt
     5521 gaggaatatc tccatctcaa tgacatgaaa gaggcagtcc agtgcgtgca ggagctggcc
     5581 tcaccctcct tgctcttcat ctttgtacgg catggtgtcg agtctacgct ggagcgcagt
     5641 gccattgctc gtgagcatat ggggcagctg ctgcaccagc tgctctgtgc tgggcatctg
     5701 tctactgctc agtactacca agggttgtat gaaatcttgg aattggctga ggacatggaa
     5761 attgacatcc cccacgtgtg gctctaccta gcggaactgg taacacccat tctgcaggaa
     5821 ggtggggtgc ccatggggga gctgttcagg gagattacaa agcctctgag accgttgggc
     5881 aaagctgctt ccctgttgct ggagatcctg ggcctcctgt gcaaaagcat gggtcctaaa
     5941 aaggtgggga cgctgtggcg agaagccggg cttagctgga aggaatttct acctgaaggc
     6001 caggacattg gtgcattcgt cgctgaacag aaggtggagt ataccctggg agaggagtcg
     6061 gaagcccctg gccagagggc actcccctcc gaggagctga acaggcagct ggagaagctg
     6121 ctgaaggagg gcagcagtaa ccagcgggtg ttcgactgga tagaggccaa cctgagtgag
     6181 cagcagatag tatccaacac gttagttcga gccctcatga cggctgtctg ctattctgca
     6241 attatttttg agactcccct ccgagtggac gttgcagtgc tgaaagcgcg agcgaagctg
     6301 ctgcagaaat acctgtgtga cgagcagaag gagctacagg cgctctacgc cctccaggcc
     6361 cttgtagtga ccttagaaca gcctcccaac ctgctgcgga tgttctttga cgcactgtat
     6421 gacgaggacg tggtgaagga ggatgccttc tacagttggg agagtagcaa ggaccccgct
     6481 gagcagcagg gcaagggtgt ggcccttaaa tctgtcacag ccttcttcaa gtggctccgt
     6541 gaagcagagg aggagtctga ccacaactga tgacagcttt cttgtacaaa gtggtgtgac
     6601 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6661 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6721 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6781 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6841 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6901 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6961 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7021 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7081 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7141 cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct
     7201 tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     7261 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     7321 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     7381 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     7441 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     7501 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     7561 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     7621 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     7681 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     7741 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     7801 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     7861 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     7921 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     7981 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     8041 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     8101 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     8161 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     8221 aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     8281 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     8341 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8401 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8461 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     8521 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8581 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8641 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8701 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8761 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8821 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9121 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9361 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9601 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9661 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9721 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9781 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9841 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9901 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9961 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10021 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10081 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10141 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10201 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10261 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10321 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10381 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10441 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10501 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10561 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10621 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10681 acgtc
//
