LOCUS       Exported                6264 bp ds-DNA     circular SYN 23-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    G5.pET SUMO EIF4G1+MIC 1-200
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 23, 2020 from SnapGene Viewer 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    297..299
                     /locus_tag="New Feature"
                     /label=New Feature
                     /note="New Feature"
     misc_feature    309..329
                     /locus_tag="HIS-tag"
                     /label=HIS-tag
                     /note="HIS-tag"
     misc_feature    654..665
                     /locus_tag="polylinker"
                     /label=polylinker
                     /note="polylinker"
     misc_feature    666..1265
                     /locus_tag="EIF4G 1-200"
                     /label=EIF4G 1-200
                     /note="EIF4G 1-200"
     misc_feature    810..830
                     /locus_tag="MIC"
                     /label=MIC
                     /note="MIC"
     misc_feature    1266..1274
                     /locus_tag="polylinker(1)"
                     /label=polylinker(1)
                     /note="polylinker(1)"
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
      241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatgg
      301 gcagcagcca tcatcatcat catcacggca gcggcctggt gccgcgcggc agcgctagca
      361 tgtcggactc agaagtcaat caagaagcta agccagaggt caagccagaa gtcaagcctg
      421 agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc aagatcaaaa
      481 agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt aaggaaatgg
      541 actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc cctgaagatt
      601 tggacatgga ggataacgat attattgagg ctcacagaga acagattggt ggtagtactg
      661 gatccaacaa ggctccgcag agcactggtc cgcctccggc accttccccg gggcttcctc
      721 agccggcttt tccgcccggg cagaccgctc ccgttgtttt cagtactccg caagctaccc
      781 aaatgaacac tccgtctcag ccgcggcagg gaggatttcg gtctctgcag catttctacc
      841 cgagcagagc acagccgccc agctcggctg caagcagagt tcagtcggct gcaccggcaa
      901 gaccgggccc ggctgcacac gtatacccgg caggcagcca agtaatgatg atcccgagcc
      961 agatcagcta cccggcaagc cagggagcat actacatccc gggacaggga aggagcacgt
     1021 acgtagtccc cactcagcag tacccggttc agccgggagc accgggtttt tacccggggg
     1081 ctagcccgac tgaattcgga acatacgctg gcgcatacta cccggcacaa ggagttcagc
     1141 agttcccgac tggcgttgca ccgacaccgg tactcatgaa ccagccgccg cagatagctc
     1201 cgaaacggga aaggaaaact atcagaataa gagacccgaa ccaaggagga aaagacatca
     1261 cttaactcga gtgaagacaa gcttaggtat ttattcggcg caaagtgcgt cgggtgatgc
     1321 tgccaactta gtcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
     1381 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     1441 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
     1501 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1561 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     1621 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1681 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1741 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1801 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1861 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1921 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     1981 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2041 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     2101 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     2161 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     2221 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     2281 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     2341 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     2401 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     2461 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2521 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     2581 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2641 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2701 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2761 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2821 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta
     2881 ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt
     2941 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3001 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3061 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3121 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3601 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3661 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3721 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     3781 ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct
     3841 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     3901 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     3961 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4021 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     4081 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     4141 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     4201 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     4261 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     4321 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     4381 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     4441 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     4501 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     4561 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     4621 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga
     4681 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     4741 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     4801 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     4861 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     4921 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct
     4981 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5041 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     5101 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
     5161 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     5221 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
     5281 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     5341 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     5401 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     5461 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
     5521 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
     5581 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
     5641 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
     5701 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
     5761 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
     5821 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
     5881 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
     5941 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     6001 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     6061 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     6121 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     6181 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     6241 cgccgccgca aggaatggtg catg
//
