LOCUS       WP_0687128              6367 bp    DNA     circular
ACCESSION   WP_068712879_1
VERSION     WP_068712879_1.1
KEYWORDS    .
FEATURES             Location/Qualifiers
     terminator      35..163
                     /label=T7_terminator(1)
                     /ApEinfo_revcolor="#fffa4d"
                     /ApEinfo_label="T7_terminator"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}1}width
                     5 offset 0"
                     /ApEinfo_fwdcolor="#fffa4d"
     source          complement(1..5293)
                     /label=source:pET-28 b (+)
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /mol_type="other DNA"
                     /organism="pET-28 b (+)"
     gene            3344..3619
                     /label=tet(852-576)
                     /ApEinfo_fwdcolor="#10ff3d"
                     /ApEinfo_revcolor="#10ff3d"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /gene="tet(852-576)"
     misc_feature    2662..2684
                     /label=pGEX_3_primer
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /ApEinfo_fwdcolor="#19fff6"
                     /ApEinfo_revcolor="#19fff6"
     misc_feature    5234..5236
                     /label=start codon
                     /ApEinfo_fwdcolor="#1723ff"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}1}width
                     5 offset 0"
                     /ApEinfo_revcolor="#1723ff"
     CDS             complement(3678..4637)
                     /label=ORF frame 1
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}1}width
                     5 offset 0"
                     /ApEinfo_revcolor="#0bce3f"
                     /translation="AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVP
                     ALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWH
                     KYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITES
                     GLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQL
                     LPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /ApEinfo_fwdcolor="#0bce3f"
     terminator      35..163
                     /label=T7_terminator
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}1}width
                     5 offset 0"
                     /ApEinfo_revcolor="#fffa4d"
                     /ApEinfo_fwdcolor="#fffa4d"
     promoter        5147..5165
                     /label=T7_promoter
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}1}width
                     5 offset 0"
                     /ApEinfo_revcolor="#f00cff"
                     /ApEinfo_fwdcolor="#f00cff"
     rep_origin      1644..2263
                     /label=pBR322_origin
                     /ApEinfo_revcolor="#8d8bff"
                     /ApEinfo_fwdcolor="#8d8bff"
                     /ApEinfo_graphicformat="rrow_data {{} {} 1} width 5offset0"
     misc_feature    5165..5192
                     /label=lacO
                     /ApEinfo_fwdcolor="#19fff6"
                     /ApEinfo_revcolor="#19fff6"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
     CDS             complement(723..1538)
                     /label=KanR2
                     /translation="SHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     KTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLV
                     DASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGR
                     VGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /note="ORF frame 1"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}1}width
                     5 offset 0"
                     /ApEinfo_revcolor="#0bce3f"
                     /gene="KanR2"
                     /ApEinfo_fwdcolor="#0bce3f"
     rep_origin      192..498
                     /label=f1_origin
                     /ApEinfo_fwdcolor="#8d8bff"
                     /ApEinfo_graphicformat="rrow_data {{} {} 1} width 5offset0"
                     /ApEinfo_revcolor="#8d8bff"
     misc_feature    complement(3678..4769)
                     /label=lacI
                     /ApEinfo_fwdcolor="#19fff6"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /ApEinfo_revcolor="#19fff6"
     misc_feature    5210..5226
                     /label=T7_transl_en_RBS
                     /ApEinfo_fwdcolor="#19fff6"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /ApEinfo_revcolor="#19fff6"
     misc_feature    complement(2678..2869)
                     /label=ROP
                     /ApEinfo_fwdcolor="#19fff6"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /ApEinfo_revcolor="#19fff6"
     misc_feature    5294..6367
                     /label=WP_068712879_1
     gene            complement(723..1538)
                     /label=KanR2(1)
                     /ApEinfo_revcolor="#10ff3d"
                     /gene="KanR2"
                     /ApEinfo_fwdcolor="#10ff3d"
                     /ApEinfo_label="KanR2"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
     misc_feature    5293
                     /label=Xpress_fwd_primer
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /ApEinfo_revcolor="#19fff6"
                     /ApEinfo_fwdcolor="#19fff6"
     misc_binding    5246..5263
                     /label=His-tag
                     /ApEinfo_fwdcolor="#037aff"
                     /ApEinfo_graphicformat="rrow_data {{} {} 1} width 5offset0"
                     /ApEinfo_revcolor="#037aff"
     misc_feature    complement(77..95)
                     /label=T7_Terminal_primer
                     /ApEinfo_fwdcolor="#19fff6"
                     /ApEinfo_revcolor="#19fff6"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
     misc_feature    7..24
                     /label=6xHis
                     /ApEinfo_revcolor="#19fff6"
                     /ApEinfo_fwdcolor="#19fff6"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
     gene            4852..5115
                     /label=tet(564-300)
                     /gene="tet(564-300)"
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /ApEinfo_fwdcolor="#10ff3d"
                     /ApEinfo_revcolor="#10ff3d"
     misc_feature    5044..5063
                     /label=pBRrevBam_primer
                     /ApEinfo_graphicformat="rrow_data {{0 1 2 0 0 -1} {}0}width
                     5 offset 0"
                     /ApEinfo_fwdcolor="#19fff6"
                     /ApEinfo_revcolor="#19fff6"
ORIGIN
        1 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
       61 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      121 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      181 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      241 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      301 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      361 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      421 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      481 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      541 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
      601 attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg
      661 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat
      721 tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
      781 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
      841 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
      901 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
      961 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     1021 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     1081 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     1141 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     1201 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
     1261 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     1321 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     1381 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     1441 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     1501 ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     1561 atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt tttcgttcca
     1621 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1681 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1741 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1801 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1861 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1921 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1981 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2041 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2101 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2161 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2221 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2281 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2341 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2401 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2461 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2521 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2581 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2641 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2701 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2761 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2821 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2881 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2941 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     3001 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     3061 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     3121 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     3181 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     3241 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     3301 tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg
     3361 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt
     3421 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt
     3481 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga
     3541 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
     3601 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta
     3661 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3721 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt
     3781 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
     3841 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
     3901 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
     3961 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
     4021 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
     4081 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
     4141 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg
     4201 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
     4261 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
     4321 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
     4381 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
     4441 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg
     4501 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg
     4561 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg
     4621 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg
     4681 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
     4741 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
     4801 gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt
     4861 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     4921 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     4981 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     5041 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     5101 cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
     5161 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
     5221 aaggagatat accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc
     5281 gcgcggcagc catatgaaat tctcacagac gtatatcgaa agtctggcag ctgcgctgcc
     5341 agatgaaatt cttactagtg cagagatcga gcgccggctg gcgccgctgt atgaacgcct
     5401 gcacctgccc gaaggtcgcc tcgaactgat gaccggcatt agggagagac gtctgtggcc
     5461 tgcaggtact cgtgcttcag atgcctctgc tcgggcgggt agagccgcac tggcgaaatc
     5521 atgcattcgg cctgaacaga ttgagctgta catccatgcg gctgtgtgtc gggatatgct
     5581 ggaaccagcc acagcggcat ttgctcacag gaaaatcggc ttatcaccga actgccagat
     5641 ttttgacttg tccaatgcgt gcctcgggtt cctcaatgca ttaactcttg ctgcatctat
     5701 gatcgaggcg ggacagattc gcgccgcatt aattacttgc ggcgaagatt ccagaccact
     5761 tgttgaacag accctccgca ctctgaacgc cgagccactg acgcgtaatg gtatcaaacc
     5821 ctattttgcc aatctgaccg tagggtcagg ggcggtgggc gctgtcgtct gccacgaaag
     5881 tcttgtggtt ccggagggtc tgggcagtgg tggtgccgca ggcgaatcga gccgtgctca
     5941 ccgccttatc ggtggggtgg tccgtgccgc aacagcccat agtgagcttt gccagggcga
     6001 aacgcatggg gcagatagcc tcgccatgca gacagacagc gaggccttac taatagccgg
     6061 agttactttg gcgcacgaaa cctggggtca gttctgcgaa gaaaccggat gggacgcagc
     6121 taccccgtct cgttttattt gccatcaagt cggttcgatc catcggcgca aactttacga
     6181 tacgttaggt atagatttgg ctaaggactt ttcgactttc gagtttttgg gaaacactgg
     6241 gtcagtagca ctcccagcga cattagcact tgcggtcgaa aaaggtgttg tgcgctctgg
     6301 tgacaaggta gcattacttg gtattggcag tggtctgaat tcactgatgt tagctgtaga
     6361 atggtga
//
