LOCUS       WP_0038057              6347 bp    DNA     circular
ACCESSION   WP_003805730_1
VERSION     WP_003805730_1.1
KEYWORDS    .
FEATURES             Location/Qualifiers
     CDS             complement(3679..4638)
                     /label=ORF frame 1
                     /codon_start=1
                     /translation="AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVP
                     ALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWH
                     KYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITES
                     GLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQL
                     LPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"
     misc_feature    5045..5064
                     /label=pBRrevBam_primer
     rep_origin      1645..2264
                     /label=pBR322_origin
     misc_feature    5166..5193
                     /label=lacO
     gene            3345..3620
                     /label=tet(852-576)
                     /gene="tet(852-576)"
     misc_feature    5211..5227
                     /label=T7_transl_en_RBS
     misc_feature    complement(3679..4770)
                     /label=lacI
     misc_feature    5294
                     /label=Xpress_fwd_primer
     promoter        5148..5166
                     /label=T7 promoter
     tag             5247..5264
                     /label=6xHis
     misc_feature    5295..6347
                     /label=WP_003805730_1
     CDS             complement(724..1539)
                     /label=KanR2
                     /note="ORF frame 1"
                     /translation="SHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     KTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLV
                     DASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGR
                     VGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /codon_start=1
                     /gene="KanR2"
     source          1..5294
                     /label=synthetic DNA construct
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2663..2685
                     /label=pGEX_3_primer
     primer          5262..5294
                     /label=pET28 5' overlap
     misc_feature    8..25
                     /label=6xHis
     gene            4853..5116
                     /label=tet(564-300)
                     /gene="tet(564-300)"
     rep_origin      193..499
                     /label=f1_origin
     primer          complement(1..34)
                     /label=pET28 3' overlap
     misc_feature    complement(2679..2870)
                     /label=ROP
     terminator      36..164
                     /label=T7_terminator
     gene            complement(724..1539)
                     /label=KanR2
                     /gene="KanR2"
ORIGIN
        1 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
       61 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      121 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      181 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      241 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      301 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      361 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      421 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      481 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      541 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      601 aattttaaca aaatattaac gtttacaatt tcaggtggca cttttcgggg aaatgtgcgc
      661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
      721 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
      781 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
      841 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
      901 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
      961 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     1021 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     1081 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     1141 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     1201 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     1261 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     1321 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     1381 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     1441 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     1501 cggcctagag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     1561 tatgtaagca gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1801 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2281 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2341 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2401 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     2461 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
     2521 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     2581 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     2641 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     2701 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
     2761 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     2821 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     2881 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     2941 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     3001 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     3061 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     3121 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     3181 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     3241 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     3301 gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg
     3361 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
     3421 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
     3481 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
     3541 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
     3601 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     3661 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3721 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     3781 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     3841 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     3901 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     3961 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     4021 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     4081 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     4141 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     4201 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     4261 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     4321 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     4381 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     4441 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     4501 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     4561 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     4621 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     4681 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     4741 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     4801 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
     4861 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     4921 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     4981 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     5041 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     5101 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
     5161 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
     5221 gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc ggcctggtgc
     5281 cgcgcggcag ccatatgcag gacggcaacg agcaagggct ggacttagcg ggtaatgcaa
     5341 cgtttcgcca ttcgaatacg gcattactta gtgtgtcatc agtcgaggcg ccccggattg
     5401 ttagtagcac tgatttcgat cgcagattag cctcgaccct gcagcgtctc aaatttccac
     5461 cacgtctgct ggagcgggtg gctggaatta cgcatcgtag atggtgggca gctggcacgt
     5521 cttttgatga cgctgcggtg gaagcgggtg caaaggcgtt agccgaagcc ggagttgaag
     5581 cctccgatat cggcttactt attaatacaa gcgtgacacg gcgtaatttg gaaccgagtg
     5641 tggcggtgaa aattcatcat ggtctctcac ttccaagcag tgcaatgaac tttgatcttg
     5701 caaacgcgtg tctgggcttt gtgaatggta tgactttggc ggctaatatg attgacagcg
     5761 gccaaattaa gtatgcaatg atagttaatg gcgaagatgc tcaagctaca caagaagcaa
     5821 ccctggcacg gctgcagcgg cctgaaacca cacgggaaga ctttaataga gagttcgcta
     5881 ccttaacgtt gggtagcggg gccgctgcag cagtccttgg gccggccgat caacaccccg
     5941 gtgcgcatcg aatcctcggc ggtgtcatgc gtgcaggtac cgagcaccac gagttatgtg
     6001 ttggtggtat agacggcatg gccaccgaca ccaagggttt attggacggt ggattgcgtc
     6061 tcgtagtaga cgcttggaat gaagcacagc cagaatggga ttgggcgagc atggatcgct
     6121 atattacaca tcaggtcagt actgcataca ctcaggctat cataggcgca attgatttag
     6181 acccagatag agtgcctatc accttcccac attggggcaa tgttgggcca gcttccttgc
     6241 ctatgactct tgcggcagaa gcccagaccc tgggtacggg ggatcgcgtt ctttgcatgg
     6301 gagtgggctc gggtcttaac acagcgatgg tggagatcgt ttggtga
//
