LOCUS       Exported               10236 bp ds-DNA     circular SYN 31-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Zengpeng Li
  TITLE     Direct Submission
  JOURNAL   Exported Jul 31, 2020 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     regulatory      3123..3132
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3138..3158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3174..3194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3210..3230
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKSKV"
     CDS             3300..3683
                     /codon_start=1
                     /label=LDB-3
                     /translation="EVQLQASGGGFVQPGGSLRLSCAASGFTWDHYIMGWFRQAPGKER
                     EFVSAISENGDAWNYYADSVKGRFTISRDNSKNTVYLQMNSLRAEDTATYYCAIGFDVP
                     SGRSWQGSHFWMYWGQGTQVTVSS"
     CDS             3702..4496
                     /codon_start=1
                     /label=P65
                     /translation="QYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRI
                     AVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQA
                     PAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDED
                     LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQ
                     RPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS"
     CDS             4518..4547
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          4636..4865
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    5042..5630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5513..5524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6037..6217
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6249..6473
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6519..6947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6961..7291
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7142..7277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7339..7386
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7405..7779
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7909..8030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8079..8095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8103..8119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8127..8157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8172..8193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8481..9069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9240..10100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10101..10205)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttgccaccat gggatccccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga
     3181 aaaagcggaa agtcgaggcc tccgcatctc caaaaaaaaa aagcaaggtt gaagcatctg
     3241 gatccggtac cggaggaagt ggcagctctg gcggcagtgg agggtctggt ggcagcggag
     3301 aagttcagct gcaggcaagc ggtggtggtt ttgttcagcc tggtggtagc ctgcgtctga
     3361 gctgtgcagc cagcggtttt acctgggatc attacatcat gggctggttt cgccaggcac
     3421 cgggtaaaga acgtgaattt gttagcgcaa tcagcgaaaa tggtgatgca tggaattatt
     3481 atgccgatag cgtgaaaggt cgctttacca ttagccgtga taatagcaaa aataccgttt
     3541 acctgcagat gaatagtctg cgtgcagaag ataccgcaac ctattattgt gcaatcggtt
     3601 ttgatgttcc atctggtcgt tcttggcagg gttctcattt ttggatgtat tggggtcagg
     3661 gcacccaggt taccgttagc agcagcggcg gcggtggcag tcagtacctg ccagatacag
     3721 acgatcgtca ccggattgag gagaaacgta aaaggacata tgagaccttc aagagcatca
     3781 tgaagaagag tcctttcagc ggacccaccg acccccggcc tccacctcga cgcattgctg
     3841 tgccttcccg cagctcagct tctgtcccca agccagcacc ccagccctat ccctttacgt
     3901 catccctgag caccatcaac tatgatgagt ttcccaccat ggtgtttcct tctgggcaga
     3961 tcagccaggc ctcggccttg gccccggccc ctccccaagt cctgccccag gctccagccc
     4021 ctgcccctgc tccagccatg gtatcagctc tggcccaggc cccagcccct gtcccagtcc
     4081 tagccccagg ccctcctcag gctgtggccc cacctgcccc caagcccacc caggctgggg
     4141 aaggaacgct gtcagaggcc ctgctgcagc tgcagtttga tgatgaagac ctgggggcct
     4201 tgcttggcaa cagcacagac ccagctgtgt tcacagacct ggcatccgtc gacaactccg
     4261 agtttcagca gctgctgaac cagggcatac ctgtggcccc ccacacaact gagcccatgc
     4321 tgatggagta ccctgaggct ataactcgcc tagtgacagg ggcccagagg ccccccgacc
     4381 cagctcctgc tccactgggg gccccggggc tccccaatgg cctcctttca ggagatgaag
     4441 acttctcctc cattgcggac atggacttct cagccctgct gagtcagatc agctccctcg
     4501 agggtggtgg ttccggtgag cagaagctga tctcagagga ggacctgtga tcgagccatg
     4561 gaagcttgat atctaactga ctgaaccggt ggtaccgagg aattaattcg ctgtctgcga
     4621 gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct gcgctaagat
     4681 tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg atgcctttga
     4741 gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc ttgaggtgtg
     4801 gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg cctttctctc
     4861 cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg cggccaattc
     4921 cgccgatcct ggaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     4981 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcaga
     5041 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5101 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5161 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5221 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5281 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5341 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5401 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     5461 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5521 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5581 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg
     5641 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5701 aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     5761 ctgtggatct accacacaca aggctacttc cctgattgac agaactacac accagggcca
     5821 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     5881 aagatagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     5941 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     6001 catcacgtgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6181 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg ccgctttaaa cccgctgatc
     6241 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6301 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6361 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6421 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6481 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6661 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6721 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6841 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc
     6901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6961 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag
     7021 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7081 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7141 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7201 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7261 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7321 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     7381 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     7441 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     7501 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     7561 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     7621 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     7681 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     7741 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     7801 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     7861 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     7921 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7981 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8041 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8101 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8161 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8221 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8281 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8641 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     8701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8881 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9121 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9181 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10201 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
