LOCUS       Exported               10905 bp ds-DNA     circular SYN 27-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Zengpeng Li
  TITLE     Direct Submission
  JOURNAL   Exported Jul 31, 2020 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     regulatory      3123..3132
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3138..3578
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        3589..3607
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3621..3623
                     /codon_start=1
                     /product="start codon for in vitro 
                     transcription/translation"
                     /label=ATG
                     /translation="M"
     CDS             3627..3656
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3660..5165
                     /codon_start=1
                     /label=DrBphP
                     /translation="MSRDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQMSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD
                     WPAAGHLSLTVHRVGELLILEFEPTEAWDSTGPHALRNAMFALESAPNLRALAEVATQT
                     VRELTGFDRVMLYKFAPDATGEVIAEARREGLHAFLGHRFPASDIPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIACHHQTPYVLPPDLRTTLEYLGRLLSLQVQVKEAADVAAFRQSLREHHARVAL
                     AAAHSLSPHDTLSDPALDLLGLMRAGGLILRFEGRWQTLGEVPPAPAVDALLAWLETQP
                     GALVQTDALGQLWPAGADLAPSAAGLLAISVGEGWSECLVWLRPELRLEVAWGGATPDQ
                     AKDDLGPRHSFDTYLEEKRGYAEPWHPGEIEEAQDLRDTLTGAL"
     CDS             5187..5216
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          5305..5534
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    5711..6299
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6182..6193)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6706..6886
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6918..7142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7188..7616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7630..7960
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7811..7946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8008..8055
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8074..8448
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8578..8699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8748..8764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8772..8788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8796..8826)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8841..8862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9150..9738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9909..10769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10770..10874)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttgccaccat gggatccatg aagctactgt cttctatcga acaagcatgc gatatttgcc
     3181 gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca
     3241 actgggagtg tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga
     3301 cagaagtgga atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag
     3361 aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag
     3421 gattatttgt acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg
     3481 agactgatat gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag
     3541 agagtagtaa caaaggtcaa agacagttga ctgtatcgcc ggaatttgta atacgactca
     3601 ctatagggcg agccgccatc atggaggagc agaagctgat ctcagaggag gacctgcata
     3661 tgagtcgtga ccctttgcca ttctttcctc ctctttatct gggtggaccc gagattacaa
     3721 cagaaaactg cgaacgcgaa ccaattcaca tcccgggatc tattcaacca cacggtgcat
     3781 tgctgacggc agacggacat tccggagagg ttttacagat gtcgcttaac gcagcaacgt
     3841 ttctgggaca agagcctacg gttttgcgcg gccagacgtt agcggctctg ttgccagagc
     3901 aatggccggc cttacaggcg gcattgcctc cagggtgccc cgatgcattg caataccgcg
     3961 cgacactgga ttggccggcg gcaggacatc tttctctgac agtccaccgc gtgggcgagc
     4021 tgttgatcct ggagtttgaa cctacggagg cctgggactc gactggcccg cacgcgttac
     4081 gcaatgcgat gttcgctctt gaatcagcgc caaacttgcg cgcgttagct gaagtggcca
     4141 cacaaaccgt acgcgagctt acaggctttg accgcgtgat gttatacaaa ttcgcacccg
     4201 atgcgacagg cgaggtaatc gccgaagccc gccgcgaggg gttgcatgcc tttcttggcc
     4261 atcgttttcc ggcctcagat attcccgccc aagcgcgcgc cctttacact cgccatctgc
     4321 ttcgtttgac tgcggacacg cgcgcggcgg ccgttccctt agacccagta cttaatcctc
     4381 agactaacgc tcctaccccc ttaggggggg cagtgctgcg tgcgacgtcg cctatgcaca
     4441 tgcagtacct tcgcaatatg ggcgtcggct cctctttaag tgtatcagtg gtagttgggg
     4501 ggcagttatg gggtctgatt gcgtgccatc atcagacccc ctatgttttg ccaccagacc
     4561 ttcgtactac tcttgaatac ttggggcgtt tattaagcct tcaggtgcaa gtcaaggaag
     4621 ccgcggacgt tgctgcattc cgtcagtcac ttcgcgaaca ccatgcgcgc gtcgccttag
     4681 cggcagcgca ttccctgtcg ccgcacgata ctctttccga ccctgcactt gatcttctgg
     4741 gtctgatgcg tgctgggggc ttaatcctgc gttttgaagg tcgttggcag acgttaggag
     4801 aagtcccgcc cgctcccgca gtcgatgcac tgcttgcatg gcttgaaacc caaccagggg
     4861 cgcttgttca gactgatgca ttggggcagt tgtggccggc gggggctgat ttggctccct
     4921 cagccgcggg tctgcttgcc atttcagtag gggagggatg gagtgagtgc ttggtttggt
     4981 tacgtcccga actgcgcctt gaggttgcgt ggggtggagc aactccagac caggccaagg
     5041 acgacctggg ccctcgtcac agtttcgata cttacttaga agagaagcgt gggtatgcag
     5101 aaccctggca tcccggagag attgaggaag ctcaggattt gcgcgacact cttactggcg
     5161 cattactcga gggtggtggt tccggtgagc agaagctgat ctcagaggag gacctgtgat
     5221 cgagccatgg aagcttgata tctaactgac tgaaccggtg gtaccgagga attaattcgc
     5281 tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc atgacttctg
     5341 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
     5401 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
     5461 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     5521 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gtcgagcatg catctagggc
     5581 ggccaattcc gccgatcctg gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     5641 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     5701 cacctcagac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5761 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5821 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5881 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5941 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6001 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6061 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     6121 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6181 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6241 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6301 tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6361 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaaag aagacaagat
     6421 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattgaca gaactacaca
     6481 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     6541 gagccagata agatagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     6601 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     6661 ctagcatttc atcacgtggc ccgagagctg catccggact gtactgggtc tctctggtta
     6721 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6781 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6841 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc cgctttaaac
     6901 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     7021 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     7081 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7141 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     7201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7321 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca
     7381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7501 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg
     7561 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     7621 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag
     7681 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7741 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7801 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7861 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     7921 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     7981 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     8041 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     8101 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     8161 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     8221 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     8281 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     8341 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     8401 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     8461 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8521 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8581 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8641 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8701 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8761 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8821 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8881 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8941 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9001 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9061 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9121 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9181 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9241 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9301 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     9361 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9421 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9481 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9541 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     9601 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9661 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9721 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9781 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9841 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9901 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9961 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10021 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10081 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10141 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10201 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10261 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10321 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10381 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10441 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10501 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10561 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10621 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10681 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10741 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10801 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10861 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
