LOCUS       pAAV-hSyn-flex-TeLC-P2A 7088 bp ds-DNA     circular     29-JUL-2020
DEFINITION  .
KEYWORDS    "creator:kevinu" "marker:AmpR, AAV2 ITR, AAV2 ITR"                 
FEATURES             Location/Qualifiers
     primer_bind     complement(1114..1133)
                     /label="mCherry-F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    3036..3069
                     /label="lox2272"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     repeat_region   4351..4491
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    3140..3173
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             complement(1565..1621)
                     /label="P2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1649..3019)
                     /label="Tetanus toxin light chain"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(3686..3697)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      6439..7027
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             5408..5476
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3215..3803
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    complement(776..809)
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     polyA_signal    3835..4311
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     1383..1401
                     /label="mCherry-R"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(916..939)
                     /label="DsRed1-C"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(1532..1552)
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      4566..5021
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        5303..5407
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(833..1531)
                     /label="dTomato"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             5477..6268
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    164..648
                     /label="hSyn promoter"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     protein_bind    complement(680..713)
                     /label="lox2272"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     primer          complement(3608..3629)
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 3608-3629 REV 6"
                     /note="sequence: gccaaggaaaggacgatgattt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1113..1133)
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 1113-1133 REV 1"
                     /note="sequence: ccccgtaatgcagaagaagac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1102..1120
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 1102-1120 FWD 2"
                     /note="sequence: cccagcccatggtcttctt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1593..1614
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 1593-1614 FWD 3"
                     /note="sequence: gcttgtttcaacagggagaagt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2078..2097
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 2078-2097 FWD 4"
                     /note="sequence: gtcgttgcaggaggtaacct"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2112..2133)
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 2112-2133 REV 3"
                     /note="sequence: tgaacgactacaaagctatcgc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          579..600
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 579-600 FWD 1"
                     /note="sequence: gactcagcgctgcctcagtctg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3106..3127)
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 3106-3127 REV 5"
                     /note="sequence: agatctggactagaccctcgat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1593..1614)
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 1593-1614 REV 2"
                     /note="sequence: acttctccctgttgaaacaagc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2619..2640)
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 2619-2640 REV 4"
                     /note="sequence: aattcgacaccaactccaactc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2596..2617
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 2596-2617 FWD 5"
                     /note="sequence: gttccagcaggttgaaggaaac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          3088..3109
                     /label="pAAV-hSyn-flex-TeLC-P2A-NLS-dTomato 3088-3109 FWD 6"
                     /note="sequence: tttgatccaagcatcaccatcg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taagtgtcta gactgcagag
      181 ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt gggggtgcct
      241 acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc
      301 atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag
      361 cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca
      421 gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt cccggccacc
      481 ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag
      541 gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg
      601 cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgagaaac cggctagagg
      661 atccgccgcc accatggcca taacttcgta taaagtatcc tatacgaagt tatatcaaaa
      721 taggaagacc aatgcttcac catcgacccg aattgccaag catcaccatc gacccataac
      781 ttcgtataat gtatgctata cgaagttatt tcgcctgcag gggcgcgcct tacttgtaca
      841 gctcgtccat gccgtacagg aacaggtggt ggcggccctc ggagcgctcg tactgttcca
      901 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg tagtagtagc
      961 cgggcagttg cacgggcttc ttggccatgt agatggtctt gaactccacc aggtagtggc
     1021 cgccgtcctt cagcttcagg gcctggtgga tctcgccctt cagcacgccg tcgcgggggt
     1081 acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
     1141 gggggaagtt ggtgccgcgc atcttcacct tgtagatcag cgtgccgtcc tgcagggagg
     1201 agtcctgggt cacggtcacc agaccgccgt cctcgaagtt catcacgcgc tcccacttga
     1261 agccctcggg gaaggacagc ttcttgtaat cggggatgtc ggcggggtgc ttcacgtacg
     1321 ccttggagcc gtacatgaac tggggggaca ggatgtccca ggcgaagggc agggggccgc
     1381 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
     1441 cctcgatctc gaactcgtgg ccgttcatgg agccctccat gcgcaccttg aagcgcatga
     1501 actctttgat gacctcctcg cccttgctca ccaccttcct cttcttcttg ggggccattc
     1561 tagatggccc gggattctct tcgacatccc ctgcttgttt caacagggag aagttagtgg
     1621 ctccgcttcc gttaacagcg ctgctagcag cggtacggtt gtacaggttt tcacggatgt
     1681 tggtcggcgg gatgattttt ttgcacagac cgatcagttt ggaaaccaga ccggaaccgt
     1741 cgacgttcct aaaggcgtta gtgttcaccc tcatgttctg gcccttatac tcggacttca
     1801 gatccttgga ctctatgtta aagccctcag tatcgttata tatagtatca tccagcaggt
     1861 taggtatctt cacaggatcg tggttcatgg aaaaatagct taagcgagtc tttatgttaa
     1921 acttcttgcc cagctctatc tcagtaaagc catacattat ggagttatac agtatctgaa
     1981 acttatcctc gttcactata tactggccgt tggaatcctt atcaaactga tacttctgct
     2041 gatagatctg tttgtaggag tcgatgtcga tgttcgggtc gttgcaggag gtaacctggg
     2101 acagtttgtt agcgatagct ttgtagtcgt tcagggtttt ttcgtacagg tcgtttttga
     2161 tatcgatgga gatcaggtta gcgtcctgac caccgaaggt gaagagctct tcagcggaga
     2221 tcgggtaggt gtgctgcatg tagatttcct gtttggacgg gatgatttcg tgggaggaaa
     2281 cctgcatgcc gtacagaccg tgcagaacgt ggatcagttc gtgcatcagc agcagagccg
     2341 ggtcctggaa gtatttggat ttaccgatgg tcagggaggt gatgttttcg ataacgttgt
     2401 cgaaggtcgg tacgtattcc gggcagaaag ccatctgcat gatggaaccg aaaccgtcac
     2461 ggcacgggaa gtagtttttg ttgtcaacac gcagaacgat accacgaact tcgtttttgt
     2521 tcagaaccgg acccggaccg aagatgatca ggttggtcag catagcggat ttggtggtag
     2581 caccggacgg atcctgttcc agcaggttga aggaaacgga gttggagttg gtgtcgaatt
     2641 tgtccagcag ggagtaggag ttacccaggt acgggatagc gttgatgatt ttgtccagca
     2701 gagcttcacc agcaacgttg tttttgatac ggttgaacag tttaaccatg gtctgcagga
     2761 aacggtcttt gtcggagtcg gtacgcaggt agttcgggtc gtagtattcg gaagcacctt
     2821 cgatcaggga ggacggcggg ttgaagtctt ccggtttggt accaaattcg taacgttccg
     2881 gaacgatcca gatacggtcg gtgattttga aggctttgta gtagatgtct agacctttgc
     2941 agtacggcgg ttccatcatg atgatggtgt cgttgttaac cgggtcggag taacggaagt
     3001 tgttgatggt gatcggcata ccggtttctc gacgcataac ttcgtatagg atactttata
     3061 cgaagttatc tttgggattc ttcctatttt gatccaagca tcaccatcga ccctctagtc
     3121 cagatctcac catcgaccca taacttcgta tagcatacat tatacgaagt tatgtccctc
     3181 gaagaggttc gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3241 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3301 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3361 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3421 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3481 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3541 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3601 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     3661 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3721 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3781 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg
     3841 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     3901 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     3961 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     4021 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     4081 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     4141 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     4201 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     4261 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg
     4321 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4381 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4441 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4501 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt gaaagcaacc
     4561 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4621 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4681 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4741 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4801 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4861 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     4921 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4981 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5041 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5101 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5161 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5221 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5281 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5341 aaatatgtat ccgctcatga gagaataacc ctgataaatg cttcaataat attgaaaaag
     5401 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5461 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5521 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5581 tcgccccgaa gaactttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5641 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5701 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5761 agaattatgc agtgctgcca taaccatgag tgattacact gcggccaact tacttctgac
     5821 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5881 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5941 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6001 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6061 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6121 tgggtctcgc ggtatcattg cagcactggg gccacatggt aagccctccc gtatcgtagt
     6181 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6241 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6301 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6361 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6421 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6481 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6541 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6601 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6661 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6721 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6781 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6841 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6901 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6961 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7021 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7081 tcacatgt
//