LOCUS       pRSFDuet_sumo_ns        6711 bp    DNA     linear   SYN 07-Apr-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     source          join(1..>1426,<1427..6711)
                     /note="/dnas_title=""pRSFDuet_sumo_ns"" [Split]"
     misc_feature    974..995
                     /note="T7 promoter"
     misc_feature    993..1017
                     /note="lacO"
     misc_feature    1073..1093
                     /note="HIS"
     misc_feature    1130..1422
                     /note="SUMO"
     frag            join(1422..>1426,<1427..4226)
                     /date="07-Apr-2020"
                     /left_cutter="BamHI (13445)"
                     /left_end="5GATC"
                     /note="26887 to 29694 of Untitled 2 [Split]"
                     /right_cutter="XhoI (16253)"
                     /right_end="5TCGA"
     frag            <1427..4219
                     /date="07-Apr-2020"
                     /note="13442 to 16236 of SARS_CoV_2_nsp1_16"
     CDS             <1427..4219
                     /label="nsp12"
     frag            4220..4226
                     /date="07-Apr-2020"
                     /note="1 to 16 of Untitled 3 [Split]"
     misc_feature    4320..4338
                     /note="T7 terminator"
     misc_feature    4611..5426
                     /note="KanR"
BASE COUNT  1857 A  1520 C  1474 G  1860 T  0 OTHER
ORIGIN
        1 GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATG TCCGCACCAA
       61 CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA
      121 CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG
      181 ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT
      241 ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA
      301 GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT
      361 CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG
      421 GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA
      481 TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA
      541 CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT
      601 TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA
      661 TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT
      721 CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA
      781 CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA
      841 CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC
      901 GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC
      961 TGCATTAGGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT
     1021 GTAGAAATAA TTTTGTTTAA CTTTAATAAG GAGATATACC ATGGGCAGCA GCCATCATCA
     1081 TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAC ATGGCAAGCA TGTCGGACTC
     1141 AGAAGTCAAT CAAGAAGCTA AGCCAGAGGT CAAGCCAGAA GTCAAGCCTG AGACTCACAT
     1201 CAATTTAAAG GTGTCCGATG GATCTTCAGA GATCTTCTTC AAGATCAAAA AGACCACTCC
     1261 TTTAAGAAGG CTGATGGAAG CGTTCGCTAA AAGACAGGGT AAGGAAATGG ACTCCTTAAG
     1321 ATTCTTGTAC GACGGTATTA GAATCCAAGC TGATCAGACC CCTGAAGATT TGGACATGGA
     1381 GGATAACGAT ATTATTGAGG CTCACAGAGA ACAGATTGGT GGATCCGCTG ATGCACAATC
     1441 GTTTTTAAAC CGGGTTTGCG GTGTAAGTGC AGCCCGTCTT ACACCGTGCG GCACAGGCAC
     1501 TAGTACTGAT GTCGTATACA GGGCTTTTGA CATCTACAAT GATAAAGTAG CTGGTTTTGC
     1561 TAAATTCCTA AAAACTAATT GTTGTCGCTT CCAAGAAAAG GACGAAGATG ACAATTTAAT
     1621 TGATTCTTAC TTTGTAGTTA AGAGACACAC TTTCTCTAAC TACCAACATG AAGAAACAAT
     1681 TTATAATTTA CTTAAGGATT GTCCAGCTGT TGCTAAACAT GACTTCTTTA AGTTTAGAAT
     1741 AGACGGTGAC ATGGTACCAC ATATATCACG TCAACGTCTT ACTAAATACA CAATGGCAGA
     1801 CCTCGTCTAT GCTTTAAGGC ATTTTGATGA AGGTAATTGT GACACATTAA AAGAAATACT
     1861 TGTCACATAC AATTGTTGTG ATGATGATTA TTTCAATAAA AAGGACTGGT ATGATTTTGT
     1921 AGAAAACCCA GATATATTAC GCGTATACGC CAACTTAGGT GAACGTGTAC GCCAAGCTTT
     1981 GTTAAAAACA GTACAATTCT GTGATGCCAT GCGAAATGCT GGTATTGTTG GTGTACTGAC
     2041 ATTAGATAAT CAAGATCTCA ATGGTAACTG GTATGATTTC GGTGATTTCA TACAAACCAC
     2101 GCCAGGTAGT GGAGTTCCTG TTGTAGATTC TTATTATTCA TTGTTAATGC CTATATTAAC
     2161 CTTGACCAGG GCTTTAACTG CAGAGTCACA TGTTGACACT GACTTAACAA AGCCTTACAT
     2221 TAAGTGGGAT TTGTTAAAAT ATGACTTCAC GGAAGAGAGG TTAAAACTCT TTGACCGTTA
     2281 TTTTAAATAT TGGGATCAGA CATACCACCC AAATTGTGTT AACTGTTTGG ATGACAGATG
     2341 CATTCTGCAT TGTGCAAACT TTAATGTTTT ATTCTCTACA GTGTTCCCAC CTACAAGTTT
     2401 TGGACCACTA GTGAGAAAAA TATTTGTTGA TGGTGTTCCA TTTGTAGTTT CAACTGGATA
     2461 CCACTTCAGA GAGCTAGGTG TTGTACATAA TCAGGATGTA AACTTACATA GCTCTAGACT
     2521 TAGTTTTAAG GAATTACTTG TGTATGCTGC TGACCCTGCT ATGCACGCTG CTTCTGGTAA
     2581 TCTATTACTA GATAAACGCA CTACGTGCTT TTCAGTAGCT GCACTTACTA ACAATGTTGC
     2641 TTTTCAAACT GTCAAACCCG GTAATTTTAA CAAAGACTTC TATGACTTTG CTGTGTCTAA
     2701 GGGTTTCTTT AAGGAAGGAA GTTCTGTTGA ATTAAAACAC TTCTTCTTTG CTCAGGATGG
     2761 TAATGCTGCT ATCAGCGATT ATGACTACTA TCGTTATAAT CTACCAACAA TGTGTGATAT
     2821 CAGACAACTA CTATTTGTAG TTGAAGTTGT TGATAAGTAC TTTGATTGTT ACGATGGTGG
     2881 CTGTATTAAT GCTAACCAAG TCATCGTCAA CAACCTAGAC AAATCAGCTG GTTTTCCATT
     2941 TAATAAATGG GGTAAGGCTA GACTTTATTA TGATTCAATG AGTTATGAGG ATCAAGATGC
     3001 ACTTTTCGCA TATACAAAAC GTAATGTCAT CCCTACTATA ACTCAAATGA ATCTTAAGTA
     3061 TGCCATTAGT GCAAAGAATA GAGCTCGCAC CGTAGCTGGT GTCTCTATCT GTAGTACTAT
     3121 GACCAATAGA CAGTTTCATC AAAAATTATT GAAATCAATA GCCGCCACTA GAGGAGCTAC
     3181 TGTAGTAATT GGAACAAGCA AATTCTATGG TGGTTGGCAC AACATGTTAA AAACTGTTTA
     3241 TAGTGATGTA GAAAACCCTC ACCTTATGGG TTGGGATTAT CCTAAATGTG ATAGAGCCAT
     3301 GCCTAACATG CTTAGAATTA TGGCCTCACT TGTTCTTGCT CGCAAACATA CAACGTGTTG
     3361 TAGCTTGTCA CACCGTTTCT ATAGATTAGC TAATGAGTGT GCTCAAGTAT TGAGTGAAAT
     3421 GGTCATGTGT GGCGGTTCAC TATATGTTAA ACCAGGTGGA ACCTCATCAG GAGATGCCAC
     3481 AACTGCTTAT GCTAATAGTG TTTTTAACAT TTGTCAAGCT GTCACGGCCA ATGTTAATGC
     3541 ACTTTTATCT ACTGATGGTA ACAAAATTGC CGATAAGTAT GTCCGCAATC TACAACACAG
     3601 ACTTTATGAG TGTCTCTATA GAAATAGAGA TGTTGACACA GACTTTGTGA ATGAGTTTTA
     3661 CGCATATTTG CGTAAACATT TCTCAATGAT GATACTCTCT GACGATGCTG TTGTGTGTTT
     3721 CAATAGCACT TATGCATCTC AAGGTCTAGT GGCTAGCATA AAGAACTTTA AGTCAGTTCT
     3781 TTATTATCAA AACAATGTTT TTATGTCTGA AGCAAAATGT TGGACTGAGA CTGACCTTAC
     3841 TAAAGGACCT CATGAATTTT GCTCTCAACA TACAATGCTA GTTAAACAGG GTGATGATTA
     3901 TGTGTACCTT CCTTACCCAG ATCCATCAAG AATCCTAGGG GCCGGCTGTT TTGTAGATGA
     3961 TATCGTAAAA ACAGATGGTA CACTTATGAT TGAACGGTTC GTGTCTTTAG CTATAGATGC
     4021 TTACCCACTT ACTAAACATC CTAATCAGGA GTATGCTGAT GTCTTTCATT TGTACTTACA
     4081 ATACATAAGA AAGCTACATG ATGAGTTAAC AGGACACATG TTAGACATGT ATTCTGTTAT
     4141 GCTTACTAAT GATAACACTT CAAGGTATTG GGAACCTGAG TTTTATGAGG CTATGTACAC
     4201 ACCGCATACA GTCTTACAGT GACCGCTCGA GTCTGGTAAA GAAACCGCTG CTGCGAAATT
     4261 TGAACGCCAG CACATGGACT CGTCTACTAG CGCAGCTTAA TTAACCTAGG CTGCTGCCAC
     4321 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT
     4381 GCTGAAACCT CAGGCATTTG AGAAGCACAC GGTCACACTG CTTCCGGTAG TCAATAAACC
     4441 GGTAAACCAG CAATAGACAT AAGCGGCTAT TTAACGACCC TGCCCTGAAC CGACGACAAG
     4501 CTGACGACCG GGTCTCCGCA AGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT
     4561 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAATTAATTC TTAGAAAAAC
     4621 TCATCGAGCA TCAAATGAAA CTGCAATTTA TTCATATCAG GATTATCAAT ACCATATTTT
     4681 TGAAAAAGCC GTTTCTGTAA TGAAGGAGAA AACTCACCGA GGCAGTTCCA TAGGATGGCA
     4741 AGATCCTGGT ATCGGTCTGC GATTCCGACT CGTCCAACAT CAATACAACC TATTAATTTC
     4801 CCCTCGTCAA AAATAAGGTT ATCAAGTGAG AAATCACCAT GAGTGACGAC TGAATCCGGT
     4861 GAGAATGGCA AAAGTTTATG CATTTCTTTC CAGACTTGTT CAACAGGCCA GCCATTACGC
     4921 TCGTCATCAA AATCACTCGC ATCAACCAAA CCGTTATTCA TTCGTGATTG CGCCTGAGCG
     4981 AGACGAAATA CGCGGTCGCT GTTAAAAGGA CAATTACAAA CAGGAATCGA ATGCAACCGG
     5041 CGCAGGAACA CTGCCAGCGC ATCAACAATA TTTTCACCTG AATCAGGATA TTCTTCTAAT
     5101 ACCTGGAATG CTGTTTTCCC GGGGATCGCA GTGGTGAGTA ACCATGCATC ATCAGGAGTA
     5161 CGGATAAAAT GCTTGATGGT CGGAAGAGGC ATAAATTCCG TCAGCCAGTT TAGTCTGACC
     5221 ATCTCATCTG TAACATCATT GGCAACGCTA CCTTTGCCAT GTTTCAGAAA CAACTCTGGC
     5281 GCATCGGGCT TCCCATACAA TCGATAGATT GTCGCACCTG ATTGCCCGAC ATTATCGCGA
     5341 GCCCATTTAT ACCCATATAA ATCAGCATCC ATGTTGGAAT TTAATCGCGG CCTAGAGCAA
     5401 GACGTTTCCC GTTGAATATG GCTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     5461 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     5521 GGCATGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT CGTTCGACTG
     5581 CGGCGAGCGG TGTCAGCTCA CTCAAAAGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     5641 AAAGCCGGAA AGAACATGTG AGCAAAAAGC AAAGCACCGG AAGAAGCCAA CGCCGCAGGC
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGCCAGAG
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TTGGTATCTC AGTTCGGTGT AGGTCGTTCG
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAT
     6061 TGGTAACTGA TTTAGAGGAC TTTGTCTTGA AGTTATGCAC CTGTTAAGGC TAAACTGAAA
     6121 GAACAGATTT TGGTGAGTGC GGTCCTCCAA CCCACTTACC TTGGTTCAAA GAGTTGGTAG
     6181 CTCAGCGAAC CTTGAGAAAA CCACCGTTGG TAGCGGTGGT TTTTCTTTAT TTATGAGATG
     6241 ATGAATCAAT CGGTCTATCA AGTCAACGAA CAGCTATTCC GTTACTCTAG ATTTCAGTGC
     6301 AATTTATCTC TTCAAATGTA GCACCTGAAG TCAGCCCCAT ACGATATAAG TTGTAATTCT
     6361 CATGTTAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG
     6421 CATCGGTCGA GATCCCGGTG CCTAATGAGT GAGCTAACTT ACATTAATTG CGTTGCGCTC
     6481 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
     6541 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA
     6601 CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA
     6661 CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC G
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