LOCUS       pEXPqcxip-hSURF4        8091 bp    DNA     circular     04-AUG-2020
Submitted to Addgene
FEATURES             Location/Qualifiers
     misc_feature    2141..2164
                     /dnas_title="pQCXIP For"
                     /note="N982_pQCXI For"
     misc_feature    2283..3204
                     /note="Insert (in vector Bam site)"
     misc_feature    2299..2323
                     /note="attB1"
     misc_feature    complement(3137..3161)
                     /note="attB2"
     misc_feature    complement(3240..3263)
                     /dnas_title="N983_pQCXI Rev"
                     /note="pQCXIP Rev "
     misc_feature    3163..3186
                     /note="FLAG eptitope"
     misc_feature    2292..2298
                     /note="BspQi"
     misc_feature    join(2299..2323,3137..3161)
                     /dnas_title="attBs / insert"
                     /note="attBs / insert"
     misc_feature    3218..3791
                     /note="IRES"
     misc_feature    complement(3204..3219)
                     /note="N986_pQCXIrev3"
     misc_feature    2223..2225
                     /note="in pDestQCXIPflag, not in company seq."
     misc_feature    1942..1942
                     /note=""
     PCR_primer      463..484
                     /note="Lands2x CMV Forward seq facility"
     PCR_primer      480..498
                     /note="Lands 2x N850 pEGFP-N1_F (CMV)"
     misc_feature    3824..4423
                     /note="ORF, Puro"
     PCR_primer      2062..2082
                     /dnas_title="Lands 2x CMVf"
                     /note="Lands 2x CMVf facility dnaseq"
     misc_feature    2079..2097
                     /note="Lands2x N850"
     PCR_primer      2108..2132
                     /dnas_title="pLNCX5'"
                     /note="N861  pLNCX5' (CMV) 65.6 %C2%BAC"
     misc_feature    446..513
                     /note="partial CMV"
     misc_feature    3205..3215
                     /dnas_title="MCS"
                     /note=" MCS Noti Agei (BsiWi) Paci BamHi EcoRi"
     misc_feature    2326..3132
                     /note="CDS SURF4 nm_033161.4"
     misc_feature    2242..2282
                     /note="MCS"
     source          1..8091
                     /dnas_title="pEXPqcxip-hSURF4-FLAG"
ORIGIN      
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gaCGCAAATG GGCGGTAGGC
      481 GTGTACGGTG GGAGGTCTat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cgGACctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga
     2281 tcGGAGCGGC CGCTCTTCAC AAGTTTGTAC AAAAAAGCtG GCaCCATGGG CCAGAACGAC
     2341 CTGATGggca cggccgagga cttcgccgac cagttcctcc gtgtcacaaa gcagtacctg
     2401 ccccacgtgg cgcgcctctg tctgatcagc accttcctgg aggacggcat ccgtatgtgg
     2461 ttccagtgga gcgagcagcg cgactacatc gacaccacct ggaactgcgg ctacctgctg
     2521 gcctcgtcct tcgtcttcct caacttgctg ggacagctga ctggctgcgt cctggtgttg
     2581 agcaggaact tcgtgcagta cgcctgcttc gggctctttg gaatcatagc tctgcagacg
     2641 attgcctaca gcattttatg ggacttgaag tttttgatga ggaacctggc cctgggagga
     2701 ggcctgttgc tgctcctagc agaatcccgt tctgaaggga agagcatgtt tgcgggcgtc
     2761 cccaccatgc gtgagagctc ccccaaacag tacatgcagc tcggaggcag ggtcttgctg
     2821 gttctgatgt tcatgaccct ccttcacttt gacgccagct tcttttctat tgtccagaac
     2881 atcgtgggca cagctctgat gattttagtg gccattggtt ttaaaaccaa gctggctgct
     2941 ttgactcttg ttgtgtggct ctttgccatc aacgtatatt tcaacgcctt ctggaccatt
     3001 ccagtctaca agcccatgca tgacttcctg aaatacgact tcttccagac catgtcggtg
     3061 attgggggct tgctcctggt ggtggccctg ggccctgggg gtgtctccat ggatgaGAAG
     3121 AAGAAGGAGT GGGGCGaccc aGCTTTCTTG TACAAAGTGG TGGATTACAA GGATGACGAC
     3181 GATAAGTGAT TCGAAGGATC CTCCgatccg gaattccgcc cctctccctc ccccccccct
     3241 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3301 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3361 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3421 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3481 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3541 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3601 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3661 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     3721 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3781 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc
     3841 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     3901 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     3961 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4021 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4081 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4141 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4201 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4261 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4321 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa
     4381 ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     4441 gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc gacccgggcg
     4501 gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagtcgagg gctgcagcgc
     4561 tgcagaggcc gagtgcagaa ctgctccaaa gggacctcaa ggctttccga gggacactag
     4621 gctgactcca tcgagccagt gtagagataa gcttatcgat tagtccaatt tgttaaagac
     4681 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
     4741 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     4801 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     4861 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacaggg
     4921 tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     4981 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     5041 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     5101 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     5161 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     5221 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
     5281 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5341 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5401 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     5461 agcggagtgt agatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5521 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5581 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5641 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5701 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5761 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5821 cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     5881 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     5941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6541 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     7201 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7261 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7321 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7381 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7441 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     7501 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7561 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7621 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7681 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7861 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7921 cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt
     7981 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
     8041 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag t
//

