LOCUS       P1443pEXPqcxipFL        8736 bp    DNA     circular     05-AUG-2020
=Gengank entry pEXPqxcip-hCCDC47-FLAG
FEATURES             Location/Qualifiers
     misc_feature    2141..2164
                     /note="N982_pQCXI For"
     misc_feature    2283..3849
                     /note="Insert (in vector Bam site)"
     misc_feature    complement(3885..3908)
                     /note="N983_pQCXI Rev "
     misc_feature    3808..3831
                     /note="FLAG eptitope"
     misc_feature    2292..2298
                     /note="BspQi"
     misc_feature    complement(3883..3905)
                     /note="N985_pQCXIrev2"
     misc_feature    3863..4436
                     /note="IRES"
     misc_feature    complement(3849..3864)
                     /note="N986_pQCXIrev3"
     misc_feature    2223..2225
                     /dnas_title="in pDestQCXIPflag, not in Clontech seq."
                     /note="in pDestQCXIPflag, not in company seq."
     misc_feature    1942..1942
                     /note=""
     PCR_primer      463..484
                     /dnas_title="Lands2x CMV Forward seq facility"
                     /note="Lands2x CMV Forward seq facility"
     PCR_primer      480..498
                     /dnas_title="Lands 2x N850 "
                     /note="Lands 2x N850 pEGFP-N1_F (CMV)"
     misc_feature    4469..5068
                     /note="ORF, Puro"
     PCR_primer      2062..2082
                     /note="Lands 2x CMVf facility dnaseq"
     misc_feature    2079..2097
                     /dnas_title="Lands2x N850"
                     /note="Lands2x N850"
     PCR_primer      2108..2132
                     /dnas_title="N861  pLNCX5'"
                     /note="N861  pLNCX5' (CMV) 65.6 %C2%BAC"
     misc_feature    446..513
                     /note="partial CMV"
     misc_feature    2045..2112
                     /note="partial cmv"
     misc_feature    3850..3860
                     /note=" MCS Noti Agei (BsiWi) Paci BamHi EcoRi"
     misc_feature    complement(3782..3806)
                     /note="attB2"
     misc_feature    2329..3777
                     /note="CDS CCDC47 BC008905"
     misc_feature    2299..2323
                     /note="attB1"
     source          1..8736
                     /dnas_title="P1443pEXPqcxipFLAG_CCDC47"
ORIGIN      
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gaCGCAAATG GGCGGTAGGC
      481 GTGTACGGTG GGAGGTCTat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cgGACctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga
     2281 tcGGAGCGGC CGCTCTTCAC AAGTTTGTAC AAAAAAGCag gctcCACCAT GAAAGCCTTC
     2341 CACACTTTCT GTGTTGTCCT TCTGGTGTTT GGGAGTGTCT CTGAAGCCAA GTTTGATGAT
     2401 TTTGAGGATG AGGAGGACAT AGTAGAGTAT GATGATAATG ACTTCGCTGA ATTTGAGGAT
     2461 GTCATGGAAG ACTCTGTTAC TGAATCTCCT CAACGGGTCA TAATCACTGA AGATGATGAA
     2521 GATGAGACCA CTGTGGAGTT GGAAGGGCAG GATGAAAACC AAGAAGGAGA TTTTGAAGAT
     2581 GCAGATACCC AGGAGGGAGA TACTGAGAGT GAACCATATG ATGATGAAGA ATTTGAAGGT
     2641 TATGAAGACA AACCAGATAC TTCTTCTAGC AAAAATAAAG ACCCAATAAC GATTGTTGAT
     2701 GTTCCTGCAC ACCTCCAGAA CAGCTGGGAG AGTTATTATC TAGAAATTTT GATGGTGACT
     2761 GGTCTGCTTG CTTATATCAT GAATTACATC ATTGGGAAGA ATAAAAACAG TCGCCTTGCA
     2821 CAGGCCTGGT TTAACACTCA TAGGGAGCTT TTGGAGAGCA ACTTTACTTT AGTGGGGGAT
     2881 GATGGAACTA ACAAAGAAGC CACAAGCACA GGAAAGTTGA ACCAGGAGAA TGAGCACATC
     2941 TATAACCTGT GGTGTTCTGG TCGAGTGTGC TGTGAGGGCA TGCTTATCCA GCTGAGGTTC
     3001 CTCAAGAGAC AAGACTTACT GAATGTCCTG GCCCGGATGA TGAGGCCAGT GAGTGATCAA
     3061 GTGCAAATAA AAGTAACCAT GAATGATGAA GACATGGATA CCTACGTATT TGCTGTTGGC
     3121 ACACGGAAAG CCTTGGTGCG ACTACAGAAA GAGATGCAGG ATTTGAGTGA GTTTTGTAGT
     3181 GATAAACCTA AGTCTGGAGC AAAGTATGGA CTGCCGGACT CTTTGGCCAT CCTGTCAGAG
     3241 ATGGGAGAAG TCACAGACGG AATGATGGAT ACAAAGATGG TTCACTTTCT TACACACTAT
     3301 GCTGACAAGA TTGAATCTGT TCATTTTTCA GACCAGTTCT CTGGTCCAAA AATTATGCAA
     3361 GAGGAAGGTC AGCCTTTAAA GCTACCTGAC ACTAAGAGGA CACTGTTGTT TACATTTAAT
     3421 GTGCCTGGCT CAGGTAACAC TTACCCAAAG GATATGGAGG CACTGCTACC CCTGATGAAC
     3481 ATGGTGATTT ATTCTATTGA TAAAGCCAAA AAGTTCCGAC TCAACAGAGA AGGCAAACAA
     3541 AAAGCAGATA AGAACCGTGC CCGAGTAGAA GAGAACTTCT TGAAACTGAC ACATGTGCAA
     3601 AGACAGGAAG CAGCACAGTC TCGGCGGGAG GAGAAAAAAA GAGCAGAGAA GGAGCGAATC
     3661 ATGAATGAGG AAGATCCTGA GAAACAGCGC AGGCTGGAGG AGGCTGCATT GAGGCGTGAG
     3721 CAAAAGAAGT TGGAAAAGAA GCAAATGAAA ATGAAACAAA TCAAAGTGAA AGCCATGTTG
     3781 GacccaGCTT TCTTGTACAA AGTGGTGGAT TACAAGGATG ACGACGATAA GTGATTCGAA
     3841 GGATCCTCCg atccggaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     3901 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3961 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4021 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     4081 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     4141 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     4201 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     4261 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     4321 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
     4381 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt
     4441 gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc
     4501 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     4561 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     4621 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     4681 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4741 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4801 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     4861 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     4921 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     4981 cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg
     5041 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     5101 aaggagcgca cgaccccatg gctccgaccg aagccgaccc gggcggcccc gccgaccccg
     5161 cacccgcccc cgaggcccac cgactctagt cgagggctgc agcgctgcag aggccgagtg
     5221 cagaactgct ccaaagggac ctcaaggctt tccgagggac actaggctga ctccatcgag
     5281 ccagtgtaga gataagctta tcgattagtc caatttgtta aagacaggat atcagtggtc
     5341 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
     5401 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     5461 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     5521 agaatagaga agttcagatc aaggtcagga acagatggaa cagggtcgac cctagagaac
     5581 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     5641 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     5701 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     5761 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     5821 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     5881 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc
     5941 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     6001 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6061 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc
     6121 cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6181 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6241 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6301 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6361 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6421 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact
     6481 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     6541 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     6601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6661 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6721 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6781 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6841 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6901 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6961 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7021 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7081 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7141 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     7201 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7261 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7321 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7381 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7441 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7501 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7561 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7621 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7681 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7741 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7801 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     7861 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7921 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7981 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8041 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8101 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     8161 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8221 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8281 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8341 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8401 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8461 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     8521 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     8581 aattagcttg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
     8641 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     8701 ttacggggtc attagttcat agcccatata tggagt
//

