LOCUS       pHM-WI-97-puro.xdna    8696 bp      DNA     circular   8/4/20
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : AAVS1-tdTomato.gb [7807  nt] : (PacI[7622] / A
            geI[6179]) to PCR(CD274) [883  nt] : (AgeI[3] / PacI[888])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     2612..2629
                     /label=M13-fwd
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(9..29)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(7650..7670)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(397..1025)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(2473..2541)
                     /label=LacZ alpha
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(35..57)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(7676..7698)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(1177..1836)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(2076..2104)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(62..91)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(7703..7732)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      4219..4446
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2663..3463
                     /label=left arm
                     /SerialCloner_Color=&h2919C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    7844..8683
                     /label=right arm
                     /SerialCloner_Color=&hC022B0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3435..3437
                     /label=As_Cpf1_PAM
                     /SerialCloner_Color=&hC00F49
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4466..6178
                     /label=caggs_promoter
                     /SerialCloner_Color=&hC00533
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6184..7066
                     /label=hPDL1/CD274
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(CD274) [883  nt] : (AgeI[3] / PacI[888]) \ 
     misc_feature    6191..7063
                     /label=CDS
                     /SerialCloner_Color=&hC017B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 GCGGCCGCca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
       61 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
      121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
      181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
      241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
      301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
      361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
      421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
      481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
      541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
      601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
      661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
      721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
      781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
      841 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
      901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
      961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     1021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     1081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     1141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     1201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     1261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     1321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     1381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     1441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     1501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     1561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     1621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     1681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     1741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     1801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     1861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     1921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     1981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     2041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     2101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     2161 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 
     2221 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcCCTGC 
     2641 AGGtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc tgggcctgtg 
     2701 ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca gatccttccc 
     2761 tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc tctgctgtgt 
     2821 tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc acgtgatgtc 
     2881 ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc cctcacccaa 
     2941 ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg cctgtgccat 
     3001 ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc gcctcccctt 
     3061 cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc gtctccctgg 
     3121 ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc tcctgtggat 
     3181 tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta cctctctagt 
     3241 ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag agctcagcta 
     3301 gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt tgctgcctcc 
     3361 agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt taatgtggct 
     3421 ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc ttctgacctc 
     3481 ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga ggaagtcttc 
     3541 taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc gagtacaagc 
     3601 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 
     3661 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 
     3721 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 
     3781 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 
     3841 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 
     3901 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 
     3961 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 
     4021 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 
     4081 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 
     4141 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga gggcccgttt 
     4201 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 
     4261 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 
     4321 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 
     4381 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 
     4441 ctatgggtct cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt 
     4501 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 
     4561 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 
     4621 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 
     4681 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 
     4741 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 
     4801 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac 
     4861 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt 
     4921 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg 
     4981 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct 
     5041 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc 
     5101 gcggcgggcg gggagtcgct gcgacgctgc cttcgccccg tgccccgctc cgccgccgcc 
     5161 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac 
     5221 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg 
     5281 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg 
     5341 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc 
     5401 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg 
     5461 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc 
     5521 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa 
     5581 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc 
     5641 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg 
     5701 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc 
     5761 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg 
     5821 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg 
     5881 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa 
     5941 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct 
     6001 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc 
     6061 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt 
     6121 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagac 
     6181 cggtCCAACC atgAGGATAT TTGCTGTCTT TATATTCATG ACCTACTGGC ATTTGCTGAA 
     6241 CGCATTTACT GTCACGGTTC CCAAGGACCT ATATGTGGTA GAGTATGGTA GCAATATGAC 
     6301 AATTGAATGC AAATTCCCAG TAGAAAAACA ATTAGACCTG GCTGCACTAA TTGTCTATTG 
     6361 GGAAATGGAG GATAAGAACA TTATTCAATT TGTGCATGGA GAGGAAGACC TGAAGGTTCA 
     6421 GCATAGTAGC TACAGACAGA GGGCCCGGCT GTTGAAGGAC CAGCTCTCCC TGGGAAATGC 
     6481 TGCACTTCAG ATCACAGATG TGAAATTGCA GGATGCAGGG GTGTACCGCT GCATGATCAG 
     6541 CTATGGTGGT GCCGACTACA AGCGAATTAC TGTGAAAGTC AATGCCCCAT ACAACAAAAT 
     6601 CAACCAAAGA ATTTTGGTTG TGGATCCAGT CACCTCTGAA CATGAACTGA CATGTCAGGC 
     6661 TGAGGGCTAC CCCAAGGCCG AAGTCATCTG GACAAGCAGT GACCATCAAG TCCTGAGTGG 
     6721 TAAGACCACC ACCACCAATT CCAAGAGAGA GGAGAAGCTT TTCAATGTGA CCAGCACACT 
     6781 GAGAATCAAC ACAACAACTA ATGAGATTTT CTACTGCACT TTTAGGAGAT TAGATCCTGA 
     6841 GGAAAACCAT ACAGCTGAAT TGGTCATCCC AGAACTACCT CTGGCACATC CTCCAAATGA 
     6901 AAGGACTCAC TTGGTAATTC TGGGAGCCAT CTTATTATGC CTTGGTGTAG CACTGACATT 
     6961 CATCTTCCGT TTAAGAAAAG GGAGAATGAT GGATGTGAAA AAATGTGGCA TCCAAGATAC 
     7021 AAACTCAAAG AAGCAAAGTG ATACACATTT GGAGGAGACG TAATTAatTA Agatatcgtc 
     7081 gacacgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
     7141 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg 
     7201 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt 
     7261 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat 
     7321 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg 
     7381 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct 
     7441 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt 
     7501 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga 
     7561 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc 
     7621 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     7681 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     7741 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     7801 gggaaacctg tcgtgccagc ggatcgacag tactaagctt tactagggac aggattggtg 
     7861 acagaaaagc cccatcctta ggcctcctcc ttcctagtct cctgatattg ggtctaaccc 
     7921 ccacctcctg ttaggcagat tccttatctg gtgacacacc cccatttcct ggagccatct 
     7981 ctctccttgc cagaacctct aaggtttgct tacgatggag ccagagagga tcctgggagg 
     8041 gagagcttgg cagggggtgg gagggaaggg ggggatgcgt gacctgcccg gttctcagtg 
     8101 gccaccctgc gctaccctct cccagaacct gagctgctct gacgcggctg tctggtgcgt 
     8161 ttcactgatc ctggtgctgc agcttcctta cacttcccaa gaggagaagc agtttggaaa 
     8221 aacaaaatca gaataagttg gtcctgagtt ctaactttgg ctcttcacct ttctagtccc 
     8281 caatttatat tgttcctccg tgcgtcagtt ttacctgtga gataaggcca gtagccagcc 
     8341 ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag 
     8401 aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc tactcccttt ctctttctcc 
     8461 atccttcttt ccttaaagag tccccagtgc tatctgggac atattcctcc gcccagagca 
     8521 gggtcccgct tccctaaggc cctgctctgg gcttctgggt ttgagtcctt ggcaagccca 
     8581 ggagaggcgc tcaggcttcc ctgtccccct tcctcgtcca ccatctcatg cccctggctc 
     8641 tcctgcccct tccctacagg ggttcctggc tctgctctaa gggcaagggc gaattc
//