LOCUS       pDZ033_pCinLacOsO1_BCD2 6106 bp ds-DNA     circular     17-JAN-2020
DEFINITION  .
KEYWORDS    "Name:Plasmid used in strain Y of \"Predictable Tuning of Synthetic
            Microbial Consortium Pulse Generator Dynamics\"" "Full Description"
            "Reference(s), DOIs" "BTK Type" "Resistance Marker:Kanᴿ"
            "Replication Origin:pMB1 rop⁻" "Other Marker" "Sequencing Status"
FEATURES             Location/Qualifiers
     CDS             complement(4339..4947)
                     /label="rhlI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      1333..1534
                     /label="rrnb T1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    392..410
                     /label="CinR binding"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     promoter        complement(5024..5495)
                     /label="pCinLac"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     sig_peptide     complement(4306..4338)
                     /label="ssrA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    417..433
                     /label="LacOs"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5542..6106
                     /label="Spacer T7"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     RBS             473..538
                     /label="BCD2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             549..1262
                     /label="sfCFP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(5086..5104)
                     /label="cinO"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     rep_origin      1724..1925
                     /label="ROP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(5057..5062)
                     /label="-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(2334..3016)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    456..466
                     /label="LacO1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    411..416
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    446..455
                     /label="LacO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(5080..5085)
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(5462..5484)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     sig_peptide     1263..1295
                     /label="ssrA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    434..439
                     /label="-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(5063..5079)
                     /label="lacOs"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1..472
                     /label="pCinLac"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    complement(5030..5051)
                     /label="lacO1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             complement(4954..5023)
                     /label="BCD11"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3062..3874
                     /label="KanR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     terminator      complement(4066..4160)
                     /label="B0014"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_binding    12..34
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 gacgtctgtg tggaattgtg agcggataac aatttcacac agggccctcg gacaccgagg
       61 agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac
      121 ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca
      181 aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa
      241 agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg
      301 aacagctacg gaactcttgt gcgtaaggaa aagtaaggaa aacgattcct tctaacagaa
      361 atgtcctgag caatcaccta tgaactgtcg agggggccta tctgagggaa TTTACTtgtg
      421 agcgctcaca attGATACTG AGCACaattg tgagcggata acaattgcat gccacttaaa
      481 aaggagatca acaatgaaag caattttcgt actgaaacat cttaatcatg ctaaggagaa
      541 atactagtat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac
      601 tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa
      661 ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc
      721 cgactctggt aacgacgctg actTGGggtg ttcagtgctt tgctcgttat ccggaccata
      781 tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga
      841 tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata
      901 ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg
      961 gccataagct ggaatacaat TACATCagcG ACaatgttta catcaccgcc gataaacaaa
     1021 aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc
     1081 tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca
     1141 atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata
     1201 tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca
     1261 aagcagcgaa cgacgaaaat tacgcccttg cagcgtaata agaacgcaga agcggtctga
     1321 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     1381 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     1441 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1501 tgttgtttgt cggtgaacgc tctctactag agtcataagt gcggaagcgc tggcattgac
     1561 cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac
     1621 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt
     1681 tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca
     1741 aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc
     1801 tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg
     1861 accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     1921 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     1981 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     2041 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     2101 actgagagtg caccattgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2161 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2221 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2281 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2341 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2461 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2521 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2581 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2641 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2701 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2761 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2821 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2881 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2941 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3001 gggattttgg tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     3061 tatgagccat attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga
     3121 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     3181 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     3241 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     3301 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     3361 gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     3421 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     3481 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3541 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     3601 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     3661 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3721 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3781 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3841 attgcagttt catttgatgc tcgatgagtt tttctaagaa ttaatTCATG AGCGGATACA
     3901 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGGCTAG
     3961 CCGCCTGGTG CTACGCCTGA ATAAGtcatg agcggataca tatttgaatg tatttagaaa
     4021 aataaacaaa taggggttcc gcgcacattt ccccgGCTAG Ctaggaaata ataaaaaagc
     4081 cggattaata atctggcttt ttatattctc tctctagtat ataaacgcag aaaggcccac
     4141 ccgaaggtga gccagtgtga gacagcggtg cggactgttg taactcagaa taagaaatga
     4201 ggccgctcat ggcgttgact ctcagtcata gtatcgtggt atcaccggtt ggttccactc
     4261 tctgttgcgg gcaacttcag cagcacgtag gggAAGCTTt tattacgctg caagggcgta
     4321 attttcgtcg ttcgctgcgg aggtcaccgc catcgacagc ggtacgccct gcagccattc
     4381 cgggtggtag cgcagcagca tctccaggcc gcgctcctgg taggccggga agctgatcgc
     4441 gaccagcgtc tcgcccttga ccttctgcgg cgggccgagg cgctggagga tcacgccgtt
     4501 gcgaacgaaa tagcgctcca tggccgtggt ggtcaccgcc accaccgaac tggcgcccag
     4561 gtaccaggcg cattgcaggc tggaccagaa tatcttcatc gccagctgcg gatcgtccgc
     4621 cgcgctggcg gcgtagcgcg aaagctccca gaccgacgga tcgctcggcg gggtttcgct
     4681 gcacaggtag gcgaagacgt ccttgagcag gtaggcgtcg gtcgtcggca gcaggcgggc
     4741 gcaaccgcag atgccctggc ggctcatggc gacgatgtag cgggtttgcg gatggtcgaa
     4801 ctggtcgaat tcctggtcgc ggaccctgga ggtggagacc acgtcccagc ccagcttctc
     4861 gatgaagacc tgatgccggt agcgtcccag ctcggcgatc atggcggcgg aaagcccttc
     4921 cagcgattca gagagcaatt cgatcatact agtaacactc ccccgcatga ttaagatgtt
     4981 tcagtacgaa aattgctttc attgttgatc tcctttttaa gtggcatgca attgttatcc
     5041 gctcacaatt GTGCTCAGTA TCaattgtga gcgctcacaA GTAAAttccc tcagataggc
     5101 cccctcgaca gttcataggt gattgctcag gacatttctg ttagaaggaa tcgttttcct
     5161 tacttttcct tacgcacaag agttccgtag ctgttcaagt ttgtgtttca actgttctcg
     5221 tcgtttccgc aacaagtcct cttcagaaat gagcttttgc tcctctgctt ggacggacag
     5281 gatgtatgct gtggcttttt taaggataac taccttgggg gccttttcat tgttttccaa
     5341 ctccgggatc tggtcacgca gggcaaaaaa gctccgtttt agctcgttcc tcctctggcg
     5401 ctccaagacg ttgtgtgttc gcctcttgac attctcctcg gtgtccgagg gccctgtgtg
     5461 aaattgttat ccgctcacaa ttccacacag acgtcggaat tgccagctgg ggcgccctct
     5521 ggtaaggttg ggaagCTCGA Gaagcgcgtc tgaataggct tcttgtattc ctgccacaca
     5581 gggaaaccat caggagttac ccaatgcaca gcgcaacgct tgcgaagaat ctctccagtc
     5641 ttcttatctt tgacctcagc agccagcagc ttagcagcag acttaagcca gttcattgct
     5701 tcaaccgcag ctaccaccgt cacgctcaca gattcccaaa tcagcttagc catgtatcca
     5761 gcagcctgat tcggctgagt gaacatcaga cccttgccgg aatcaatagc tggctgaatg
     5821 gtatcttcca gcacttgttg acggaagccg aactctttgg acccgtaagc cagcgtcatg
     5881 actgaacgct tagtcacact gcgagtaaca ccgtaagcca gccattgacc agccagtgcc
     5941 ttagtgccca gcttgacttt ctcagagatt tcaccagtgt tctcatcggt cacggtaact
     6001 acttcgttat cggtcccatt gattgcgtct gcttgtagaa tctcgttgac tttcttagca
     6061 acaatcccgt agatgtcctg aacggtttca ctaggaagca agttaa
//