LOCUS       pDZ113_pRhl42Lac_BCD2m_ 3981 bp ds-DNA     circular     09-JUN-2020
DEFINITION  .
KEYWORDS    "Name:Plasmid used in strain Z of \"Predictable Tuning of Synthetic
            Microbial Consortium Pulse Generator Dynamics\"" "Full Description"
            "Reference(s), DOIs" "BTK Type:N/A" "Resistance Marker:Kanᴿ"
            "Replication Origin:pMB1 rop⁻" "Other Marker" "Sequencing Status"
FEATURES             Location/Qualifiers
     sig_peptide     3639..3671
                     /label="ssrA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2787..2792
                     /label="-35 (4 c)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    2793..2809
                     /label="LacOs"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        2377..2848
                     /label="pRhlLac(cC)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2810..2815
                     /label="-10 (2 C)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    2768..2786
                     /label="rhlO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      3817..3911
                     /label="B0014"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      880..1562
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    2925..3638
                     /label="sfYFP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1971..2172)
                     /label="ROP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    2388..2410
                     /label="LacO"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    2822..2831
                     /label="LacO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2832..2842
                     /label="LacO1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(22..834)
                     /label="KanR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     RBS             2849..2914
                     /label="BCD2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
       61 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      121 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      181 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      241 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
      301 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      361 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      421 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
      481 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
      541 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
      601 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
      661 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
      721 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
      781 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
      841 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
      901 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      961 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1021 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1081 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1141 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1201 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1261 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1321 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1381 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1441 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1501 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1561 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1621 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1681 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     1741 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     1801 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     1861 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     1921 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     1981 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2041 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2101 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     2161 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     2221 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2281 gagggtaaac aactggcggt atggatgcgg cgggaccaGA GCTCagtgcg CTCGAGcttc
     2341 ccaaccttac cagagggcgc cccagctggc aattccgacg tctgtgtgga attgtgagcg
     2401 gataacaatt tcacacaggg ccctcggaca ccgaggagaa tgtcaagagg cgaacacaca
     2461 acgtcttgga gcgccagagg aggaacgagc taaaacggag cttttttgcc ctgcgtgacc
     2521 agatcccgga gttggaaaac aatgaaaagg cccccaaggt agttatcctt aaaaaagcca
     2581 cagcatacat cctgtccgtc caagcagagg agcaaaagct catttctgaa gaggacttgt
     2641 tgcggaaacg acgagaacag ttgaaacaca aacttgaaca gctacggaac tcttgtgcgt
     2701 aaggaaaagt aaggaaaacg attccttcta acagaaatgt cctgagcaat cacctatgaa
     2761 ctgtcgatcc tgtgaaatct ggcagtTAGA CAtgtgagcg ctcacaattT ATAGTGAGCA
     2821 Caattgtgag cggataacaa ttgcatgcca cttaaaaagg agatcaacaa tgaaagcaat
     2881 tttcgtactg aaacatctta atcatgctaa ggagaaatac tagtatgcgt aaaggcgaag
     2941 agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata
     3001 agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt
     3061 tcatctgtac tactggtaaa ctgccggtac cttggccgac tctggtaacg acgctgactt
     3121 atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt
     3181 ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt
     3241 acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga
     3301 aaggcattga ctttaaagaa gacggcaata tcctgggcca taagctggaa tacaatttta
     3361 acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta
     3421 aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac cagcaaaaca
     3481 ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc TACcaaagcg
     3541 ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg
     3601 cagcgggcat cacgcatggt atggatgaac tgtacaaagc agcgaacgac gaaaattacg
     3661 cccttgcagc gtaataaAAG CTTcccctac gtgctgctga agttgcccgc aacagagagt
     3721 ggaaccaacc ggtgatacca cgatactatg actgagagtc aacgccatga gcggcctcat
     3781 ttcttattct gagttacaac agtccgcacc gctgtctcac actggctcac cttcgggtgg
     3841 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt
     3901 ttttattatt tcctaGCTAG Ccggggaaat gtgcgcggaa cccctatttg tttatttttc
     3961 taaatacatt caaatatgta t
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