LOCUS       pDZ134_pRhl14Tet_BCD2mo 3904 bp ds-DNA     circular     09-JUN-2020
DEFINITION  .
KEYWORDS    "Name:Plasmid used in strain Z of \"Predictable Tuning of Synthetic
            Microbial Consortium Pulse Generator Dynamics\"" "Full Description"
            "Reference(s), DOIs" "BTK Type:N/A" "Resistance Marker:Ampᴿ"
            "Replication Origin:p15A" "Other Marker" "Sequencing Status"
FEATURES             Location/Qualifiers
     misc_feature    2429..2434
                     /label="-10"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2010..2026
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    994..1924
                     /label="ampR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2406..2411
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             2468..2533
                     /label="BCD2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2544..3545
                     /label="rbsR-L"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      3727..3821
                     /label="B0014"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_binding    2387..2405
                     /label="rhlO"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     promoter        2007..2467
                     /label="pRhlTet"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      64..856
                     /label="p15A"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     sig_peptide     3546..3578
                     /label="ssrA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2442..2460
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    2412..2428
                     /label="TetO2"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 ccctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag
       61 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
      121 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
      181 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
      241 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
      301 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
      361 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
      421 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg
      541 cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc
      601 taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa
      661 gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca
      721 gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata
      781 aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc
      841 atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
      901 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
      961 agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagagcc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgtt
     1981 ccagctggca attccgacgt ctgtgtggat ccctatcagt gatagagatt cacacagggc
     2041 cctcggacac cgaggagaat gtcaagaggc gaacacacaa cgtcttggag cgccagagga
     2101 ggaacgagct aaaacggagc ttttttgccc tgcgtgacca gatcccggag ttggaaaaca
     2161 atgaaaaggc ccccaaggta gttatcctta aaaaagccac agcatacatc ctgtccgtcc
     2221 aagcagagga gcaaaagctc atttctgaag aggacttgtt gcggaaacga cgagaacagt
     2281 tgaaacacaa acttgaacag ctacggaact cttgtgcgta aggaaaagta aggaaaacga
     2341 ttccttctaa cagaaatgtc ctgagcaatc acctatgaac tgtcgatcct gtgaaatctg
     2401 gcagtTTTAC Ttccctatca gtgatagaGA TACTGAGCAC atccctatca gtgatagaga
     2461 tgcatgccac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga aacatcttaa
     2521 tcatgctaag gagaaatact agtatgAAAC CAGTAACGTT ATACGATGTc gcagagtatg
     2581 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     2641 aaacgcggga aaaagtggaa gcggcgatgg cggagctcaa ttacattccc aaccgcgtgg
     2701 cacaacaact ggcgggcaaa gcgtcgcata ccattggcat gttgatcact gccagtacca
     2761 atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa cgcggttata
     2821 gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat ctggaaacgc
     2881 tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat caaccttcgc
     2941 gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg gctccgttcg
     3001 atggcgacag cgatcttatt caggataact cgttgctggg cggagactta gcaacgcaat
     3061 atctgatcga taaaggtcat acccgtatcg cctgtattac cggcccgctg gataaaactc
     3121 cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggc ctcaacattc
     3181 ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac gctatgcgcc
     3241 aactgctatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac gctatggctg
     3301 ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat atcgcggtga
     3361 ttggCtatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc actatccacc
     3421 aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg ataacccagc
     3481 cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc ggttcggcta
     3541 cctccgcagc gaacgacgaa aattacgccc ttgcagcgTG AtaataaAAG CTTcccctac
     3601 gtgctgctga agttgcccgc aacagagagt ggaaccaacc ggtgatacca cgatactatg
     3661 actgagagtc aacgccatga gcggcctcat ttcttattct gagttacaac agtccgcacc
     3721 gctgtctcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga
     3781 atataaaaag ccagattatt aatccggctt ttttattatt tcctaGCTAG Ccggggaaat
     3841 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3901 aacg
//