LOCUS       pET_gSTEP1              6141 bp ds-DNA     circular     06-AUG-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     promoter        complement(5735..5812)
                     /label="lacI promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     6079..6094
                     /label="pET Upstream Primer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(2698..2790)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    168..192
                     /label="lac operator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(1169..1187)
                     /label="T7 Terminator Primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     124..143
                     /label="DuetUP2 Primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        149..167
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             71..73
                     /label="ATG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(3889..4080)
                     /label="rop"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      1183..1230
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    3..27
                     /label="lac operator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             1087..1131
                     /label="S-Tag"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      1267..1722
                     /label="f1 ori"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     RBS             221..226
                     /label="ribosome binding site"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(4652..5734)
                     /label="lacI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(1837..2697)
                     /label="AmpR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     RBS             58..63
                     /label="ribosome binding site"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      2871..3459
                     /label="ori"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(124..143)
                     /label="DuetDOWN1 Primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    235..1074
                     /label="gSTEP1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        join(6125..6141,1..2)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggctaag cttgcggccg cataatgctt aagtcgaaca gaaagtaatc
      121 gtattgtaca cggccgcata atcgaaatta atacgactca ctatagggga attgtgagcg
      181 gataacaatt ccccatctta gtatattagt taagtataag aaggagatat aCATATGCAT
      241 CACCACCATC ACCATGACTT GCGCCCTGAG ATTTGGATTG CACAAGAGCT GCGTCGTATT
      301 GGTGACGAAT TCAATGCGTA TTATGCGCGT CGTCACAAGA TCCGCCTGGA AAATGTTTAC
      361 ATTAAAGCCG ATAAACAGAA AAACGGCATC AAAGCAAACT TCAAGATCCG CCACAACATC
      421 GAGGATGGTG GCGTTCAGCT GGCCTATCAT TACCAGCAGA ACACCCCGAT TGGTGACGGT
      481 CCGGTCCTGC TGCCGGACAA CCACTATTTG TCCGTGCAGT CGAAGTTGAG CAAAGATCCG
      541 AATGAAAAAC GTGATCACAT GGTGCTGCTG GAGTTTGTTA CGGCTGCGGG TATTACCCTG
      601 GGTATGGATG AACTGTACAA GGGTGGTACT GGTGGCAGCA TGGTGAGCAA AGGCGAAGAA
      661 CTGTTCACGG GTGTCGTTCC GATCCTGGTT GAGTTGGACG GTGACGTTAA CGGCCACAAG
      721 TTTAGCGTGA GCGGCGAGGG TGAGGGTGAT GCGACCTATG GCAAGCTGAC CCTGAAATTC
      781 ATTTGCACCA CGGGCAAGCT GCCGGTCCCG TGGCCGACCC TGGTGACCAC CCTGACGTAC
      841 GGTGTCCAGT GCTTTTCTCG CTACCCGGAC CACATGAAGC AACATGACTT CTTTAAGTCC
      901 GCGATGCCAG AGGGCTATAT CCAAGAGCGT ACTATCTTCT TTAAAGACGA TGGTAACTAT
      961 AAGACCCGCG CTGAAGTCAA GTTCGAGGGC GATACGTTGG TCAATCGTAT TGAACTGAAG
     1021 GGCATCGATT TCAAAGAGGA CGGTAATATC CTGGGTCACA AACTGGAGTA CTGACTCGAG
     1081 tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc gtctactagc
     1141 gcagcttaat taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg
     1201 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     1261 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1321 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1381 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1441 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1501 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1561 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1621 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1681 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttctggcggc acgatggcat
     1741 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1801 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1861 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1921 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1981 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2041 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2101 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2161 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2221 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2281 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2341 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2401 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2461 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2521 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2581 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2641 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2701 cttccttttt caatcatgat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2761 tatttgaatg tatttagaaa aataaacaaa taggtcatga ccaaaatccc ttaacgtgag
     2821 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2881 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2941 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3001 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3061 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3121 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3181 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3241 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3301 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3361 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3421 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3481 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3541 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3601 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     3661 ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct
     3721 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     3781 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3841 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3901 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     3961 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     4021 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     4081 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     4141 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     4201 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     4261 ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     4321 gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     4381 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     4441 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     4501 gcctagccgg gtcctcaacg acaggagcac gatcatgcta gtcatgcccc gcgcccaccg
     4561 gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg
     4621 agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4681 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4741 gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg
     4801 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat
     4861 cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc
     4921 ccactaccga gatgtccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc
     4981 ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca
     5041 tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg
     5101 gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga
     5161 cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct
     5221 ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt
     5281 cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat
     5341 cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt
     5401 gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg
     5461 cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg
     5521 ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca
     5581 cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg
     5641 cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat
     5701 actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt
     5761 ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct
     5821 cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     5881 ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
     5941 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     6001 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
     6061 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatcgatct cgatcccgcg
     6121 aaattaatac gactcactat a
//