LOCUS       DT033A_-_pr.Tac-BDNF    2703 bp ds-DNA     circular     06-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature          751..770
                           /label="Prefix"
                           /ApEinfo_revcolor=#6ecff6
                           /ApEinfo_fwdcolor=#6ecff6
     misc_feature          complement(1399..1404)
                           /label="Scar (AT)"
                           /ApEinfo_revcolor=#f49ac2
                           /ApEinfo_fwdcolor=#f49ac2
     misc_feature          1318..1347
                           /label="polyA (30)"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     misc_feature          1225..1283
                           /label="spacer"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     misc_feature          1397..1402
                           /label="Scar (AT)"
                           /ApEinfo_revcolor=#f49ac2
                           /ApEinfo_fwdcolor=#f49ac2
     misc_feature          824..831
                           /label="spacer"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
     terminator            1348..1395
                           /label="T7 terminator"
                           /ApEinfo_revcolor=#c6c9d1
                           /ApEinfo_fwdcolor=#c6c9d1
     origin of replication 1579..2193
                           /label="rep (pMB1)"
                           /ApEinfo_revcolor=#c7b0e3
                           /ApEinfo_fwdcolor=#c7b0e3
     CDS                   complement(2356..2703)
                           /label="AmpR"
                           /ApEinfo_revcolor=#fff79a
                           /ApEinfo_fwdcolor=#fff79a
     misc_feature          859..1224
                           /label="BDNF mature"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
     misc_feature          832..858
                           /label="ATG-FLAG-tag"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     misc_feature          1284..1313
                           /label="FRET Probes"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     misc_feature          815..823
                           /label="RBS"
                           /ApEinfo_revcolor=#d59687
                           /ApEinfo_fwdcolor=#d59687
     misc_feature          1397..1417
                           /label="Suffix"
                           /ApEinfo_revcolor=#f9ad81
                           /ApEinfo_fwdcolor=#f9ad81
     terminator stem loop  722..741
                           /label="Terminator stem loop"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     misc_feature          772..806
                           /label="pr.Tac"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     RBS                   complement(76..81)
                           /label="RBS"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     CDS                   complement(1..312)
                           /label="AmpR"
                           /ApEinfo_revcolor=#fff79a
                           /ApEinfo_fwdcolor=#fff79a
     primer                1376..1417
                           /label="Suffix-T7 Term-BB-Fwd"
                           /note="sequence: gggtctTGAGGGGTTTTTTGgtactagtagcggccgctgcag"
                           /ApEinfo_revcolor=#f8d3a9
                           /ApEinfo_fwdcolor=#f8d3a9
     primer                1397..1416
                           /label="IVC BBFwd"
                           /note="sequence: tactagtagcggccgctgca"
                           /ApEinfo_revcolor=#84b0dc
                           /ApEinfo_fwdcolor=#84b0dc
     primer                749..806
                           /label="ptac 1"
                           /note="sequence: tggaattcgcggccgcttctagagttgacaattaatcatcggctcgtataatgtgtgg"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     primer                complement(1377..1396)
                           /label="IVC INRev"
                           /note="sequence: cCAAAAAACCCCTCAAGACC"
                           /ApEinfo_revcolor=#75c6a9
                           /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
       61 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      361 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
      421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
      481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
      541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
      601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
      661 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
      721 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agttgacaat
      781 taatcatcgg ctcgtataat gtgtgggata ctagaagaag gagAATAATC TATGgactac
      841 aaagacgatg atgacaagAT GCACTCCGAC CCTGCCCGCC GTGGGGAGCT GAGCGTGTGT
      901 GACAGTATTA GCGAGTGGGT CACAGCGGCA GATAAAAAGA CTGCAGTGGA CATGTCTGGC
      961 GGGACGGTCA CAGTCCTAGA GAAAGTCCCG GTATCCAAAG GCCAACTGAA GCAGTATTTC
     1021 TACGAGACCA AGTGTAATCC CATGGGTTAC ACCAAGGAAG GCTGCAGGGG CATAGACAAA
     1081 AGGCACTGGA ACTCGCAATG CCGAACTACC CAATCGTATG TTCGGGCCCT TACTATGGAT
     1141 AGCAAAAAGA GAATTGGCTG GCGATTCATA AGGATAGACA CTTCCTGTGT ATGTACACTG
     1201 ACCATTAAGA GGGGAAGAta gTAGCGACTC AGGCTGCTAC GCCTGTGTAC TGGAAAACAA
     1261 AACCAAAACC CAAAAAACAA AAAACTGAGC CCATTGGTAT CGTGGAAGGA CTCGATCAAA
     1321 AAAAAAAAAA AAAAAAAAAA AAAAAAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1381 TTGAGGGGTT TTTTGgtact agtagcggcc gctgcaggct tcctcgctca ctgactcgct
     1441 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1501 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1561 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     1621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2101 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2161 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2221 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2281 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2341 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2401 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2461 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2521 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2581 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2641 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2701 tat
//