LOCUS       DT038A_-_pr.EM-sfGFP    3057 bp ds-DNA     circular     06-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature          772..806
                           /label="pr.EMedium (J23106)"
                           /ApEinfo_revcolor=#f8d3a9
                           /ApEinfo_fwdcolor=#f8d3a9
     misc_feature          1579..1637
                           /label="spacer"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     misc_feature          1638..1667
                           /label="FRET Probes"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     terminator stem loop  722..741
                           /label="Terminator stem loop"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     misc_feature          1751..1756
                           /label="Scar (AT)"
                           /ApEinfo_revcolor=#f49ac2
                           /ApEinfo_fwdcolor=#f49ac2
     misc_feature          1751..1771
                           /label="Suffix"
                           /ApEinfo_revcolor=#f9ad81
                           /ApEinfo_fwdcolor=#f9ad81
     RBS                   complement(76..81)
                           /label="RBS"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     misc_feature          832..858
                           /label="ATG-FLAG-tag"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     CDS                   complement(2710..3057)
                           /label="AmpR"
                           /ApEinfo_revcolor=#fff79a
                           /ApEinfo_fwdcolor=#fff79a
     terminator            1702..1749
                           /label="T7 terminator"
                           /ApEinfo_revcolor=#c6c9d1
                           /ApEinfo_fwdcolor=#c6c9d1
     CDS                   859..1578
                           /label="sfGFP"
                           /ApEinfo_revcolor=#b1ff67
                           /ApEinfo_fwdcolor=#b1ff67
     misc_feature          815..823
                           /label="RBS"
                           /ApEinfo_revcolor=#d59687
                           /ApEinfo_fwdcolor=#d59687
     CDS                   complement(1..312)
                           /label="AmpR"
                           /ApEinfo_revcolor=#fff79a
                           /ApEinfo_fwdcolor=#fff79a
     origin of replication 1933..2547
                           /label="rep (pMB1)"
                           /ApEinfo_revcolor=#c7b0e3
                           /ApEinfo_fwdcolor=#c7b0e3
     misc_feature          complement(1753..1758)
                           /label="Scar (AT)"
                           /ApEinfo_revcolor=#f49ac2
                           /ApEinfo_fwdcolor=#f49ac2
     misc_feature          751..770
                           /label="Prefix"
                           /ApEinfo_revcolor=#6ecff6
                           /ApEinfo_fwdcolor=#6ecff6
     misc_feature          824..831
                           /label="spacer"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
     misc_feature          1672..1701
                           /label="polyA (30)"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     primer                complement(809..834)
                           /label="5'UTR Universal rev"
                           /note="sequence: catAGATTATTctccttcttctagta"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
     primer                633..652
                           /label="VF Primer"
                           /note="sequence: tgccacctgacgtctaagaa"
                           /ApEinfo_revcolor=#c6c9d1
                           /ApEinfo_fwdcolor=#c6c9d1
     primer                complement(1584..1603)
                           /label="spacer rev"
                           /note="sequence: TACACAGGCGTAGCAGCCTG"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     primer                1579..1612
                           /label="spacer 3'"
                           /note="sequence: CGACTCAGGCTGCTACGCCTGTGTACTGGAAAAC"
                           /ApEinfo_revcolor=#f8d3a9
                           /ApEinfo_fwdcolor=#f8d3a9
     primer                809..834
                           /label="5'UTR Universal fwd"
                           /note="sequence: tactagaagaaggagAATAATCTATG"
                           /ApEinfo_revcolor=#c6c9d1
                           /ApEinfo_fwdcolor=#c6c9d1
     primer                1579..1598
                           /label="BB Spacer Fwd"
                           /note="sequence: CGACTCAGGCTGCTACGCCT"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
       61 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      361 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
      421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
      481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
      541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
      601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
      661 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
      721 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag atttacggct
      781 agctcagtcc taggtatagt gctagcgata ctagaagaag gagAATAATC TATGgactac
      841 aaagacgatg atgacaagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
      901 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
      961 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     1021 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     1081 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     1141 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     1201 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc
     1261 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     1321 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     1381 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     1441 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     1501 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     1561 gaactgtaca aataaTAACG ACTCAGGCTG CTACGCCTGT GTACTGGAAA ACAAAACCAA
     1621 AACCCAAAAA ACAAAAAACT GAGCCCATTG GTATCGTGGA AGGACTCGAT CAAAAAAAAA
     1681 AAAAAAAAAA AAAAAAAAAA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1741 GGTTTTTTGg tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc
     1801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1921 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2581 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2641 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2701 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2761 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2821 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2881 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2941 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3001 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtat
//