LOCUS DT040A_-_pr.MW-sfGFP 3057 bp ds-DNA circular 06-AUG-2020 DEFINITION . FEATURES Location/Qualifiers terminator 1702..1749 /label="T7 terminator" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(1753..1758) /label="Scar (AT)" /ApEinfo_revcolor=#f49ac2 /ApEinfo_fwdcolor=#f49ac2 misc_feature 815..823 /label="RBS" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 1751..1756 /label="Scar (AT)" /ApEinfo_revcolor=#f49ac2 /ApEinfo_fwdcolor=#f49ac2 CDS complement(2710..3057) /label="AmpR" /ApEinfo_revcolor=#fff79a /ApEinfo_fwdcolor=#fff79a misc_feature 772..806 /label="pr.MedWeak(MW) (J23117-J23106)" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 859..1578 /label="sfGFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 832..858 /label="ATG-FLAG-tag" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd terminator stem loop 722..741 /label="Terminator stem loop" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd origin of replication 1933..2547 /label="rep (pMB1)" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1579..1637 /label="spacer" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 751..770 /label="Prefix" /ApEinfo_revcolor=#6ecff6 /ApEinfo_fwdcolor=#6ecff6 misc_feature 1672..1701 /label="polyA (30)" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac RBS complement(76..81) /label="RBS" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1751..1771 /label="Suffix" /ApEinfo_revcolor=#f9ad81 /ApEinfo_fwdcolor=#f9ad81 misc_feature 1638..1667 /label="FRET Probes" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS complement(1..312) /label="AmpR" /ApEinfo_revcolor=#fff79a /ApEinfo_fwdcolor=#fff79a misc_feature 824..831 /label="spacer" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 ORIGIN 1 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 61 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 361 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 661 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 721 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag aTTTACGGCT 781 AGCTCAGTCC TAGGGATTGT GCTAGCgata ctagaagaag gagAATAATC TATGgactac 841 aaagacgatg atgacaagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt 901 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa 961 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg 1021 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat 1081 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag 1141 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt 1201 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc 1261 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc 1321 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc 1381 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg 1441 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa 1501 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat 1561 gaactgtaca aataaTAACG ACTCAGGCTG CTACGCCTGT GTACTGGAAA ACAAAACCAA 1621 AACCCAAAAA ACAAAAAACT GAGCCCATTG GTATCGTGGA AGGACTCGAT CAAAAAAAAA 1681 AAAAAAAAAA AAAAAAAAAA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG 1741 GGTTTTTTGg tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc 1801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 1861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 1921 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca 1981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 2041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 2101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 2161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 2221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 2281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 2341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 2401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 2461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 2521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 2581 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 2641 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 2701 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 2761 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 2821 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 2881 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 2941 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 3001 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtat //