LOCUS       DT043A_-_pr.T7-sfGFP    3043 bp ds-DNA     circular     06-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature          772..794
                           /label="T7 (Registry)"
                           /ApEinfo_revcolor=#b1ff67
                           /ApEinfo_fwdcolor=#b1ff67
     misc_feature          1658..1687
                           /label="polyA (30)"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     terminator            1688..1735
                           /label="T7 terminator"
                           /ApEinfo_revcolor=#c6c9d1
                           /ApEinfo_fwdcolor=#c6c9d1
     terminator stem loop  722..741
                           /label="Terminator stem loop"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     misc_feature          1565..1623
                           /label="spacer"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     misc_feature          1737..1742
                           /label="Scar (AT)"
                           /ApEinfo_revcolor=#f49ac2
                           /ApEinfo_fwdcolor=#f49ac2
     CDS                   845..1564
                           /label="sfGFP"
                           /ApEinfo_revcolor=#b1ff67
                           /ApEinfo_fwdcolor=#b1ff67
     misc_feature          1624..1653
                           /label="FRET Probes"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     CDS                   complement(join(2696..3043,1..312))
                           /label="AmpR"
                           /ApEinfo_revcolor=#fff79a
                           /ApEinfo_fwdcolor=#fff79a
     misc_feature          1737..1757
                           /label="Suffix"
                           /ApEinfo_revcolor=#f9ad81
                           /ApEinfo_fwdcolor=#f9ad81
     misc_feature          751..770
                           /label="Prefix"
                           /ApEinfo_revcolor=#6ecff6
                           /ApEinfo_fwdcolor=#6ecff6
     misc_feature          complement(1739..1744)
                           /label="Scar (AT)"
                           /ApEinfo_revcolor=#f49ac2
                           /ApEinfo_fwdcolor=#f49ac2
     RBS                   complement(76..81)
                           /label="RBS"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     misc_feature          818..844
                           /label="ATG-FLAG-tag"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     origin of replication 1919..2533
                           /label="rep (pMB1)"
                           /ApEinfo_revcolor=#c7b0e3
                           /ApEinfo_fwdcolor=#c7b0e3
     misc_feature          801..809
                           /label="RBS"
                           /ApEinfo_revcolor=#d59687
                           /ApEinfo_fwdcolor=#d59687
     misc_feature          810..817
                           /label="spacer"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
     primer                793..865
                           /label="sfGFP-FLAG-Fwd"
                           /note="sequence: gatactagaagaaggagAATAATCTATGgactacaaagacgatgatgacaagatgcgtaaaggcgaagagctg"
                           /ApEinfo_revcolor=#d59687
                           /ApEinfo_fwdcolor=#d59687
     primer                complement(1715..1757)
                           /label="T7 Term Suffix"
                           /note="sequence: ctgcagcggccgctactagtacCAAAAAACCCCTCAAGACCCG"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     primer                1646..1705
                           /label="DT013"
                           /note="sequence: AAGGACTCGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAGCATAACCCCTTGGG"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     primer                complement(1717..1736)
                           /label="IVC INRev"
                           /note="sequence: cCAAAAAACCCCTCAAGACC"
                           /ApEinfo_revcolor=#75c6a9
                           /ApEinfo_fwdcolor=#75c6a9
     primer                complement(751..817)
                           /label="Prefix-T7-spacer-BB Rev"
                           /note="sequence: agATTATTctccttcttctagtatctccctatagtgagtcgtattatctagaagcggccgcgaattc"
                           /ApEinfo_revcolor=#f8d3a9
                           /ApEinfo_fwdcolor=#f8d3a9
     primer                1565..1584
                           /label="BB Spacer Fwd"
                           /note="sequence: CGACTCAGGCTGCTACGCCT"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
     primer                complement(795..844)
                           /label="spacer-RBS-spacer-FLAG"
                           /note="sequence: cttgtcatcatcgtctttgtagtcCATAGATTATTctccttcttctagta"
                           /ApEinfo_revcolor=#b1ff67
                           /ApEinfo_fwdcolor=#b1ff67
     primer                complement(1815..1834)
                           /label="VR Registry"
                           /note="sequence: attaccgcctttgagtgagc"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
     primer                complement(1712..1735)
                           /label="T7-TER-REV"
                           /note="sequence: CAAAAAACCCCTCAAGACCCGTTT"
                           /ApEinfo_revcolor=#c7b0e3
                           /ApEinfo_fwdcolor=#c7b0e3
     primer                1716..1757
                           /label="Suffix-T7 Term-BB-Fwd"
                           /note="sequence: gggtctTGAGGGGTTTTTTGgtactagtagcggccgctgcag"
                           /ApEinfo_revcolor=#f8d3a9
                           /ApEinfo_fwdcolor=#f8d3a9
     primer                complement(1714..1757)
                           /label="T7 Term-Suffix-Rev"
                           /note="sequence: ctgcagcggccgctactagtacCAAAAAACCCCTCAAGACCCGT"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
     primer                633..652
                           /label="VF2 Registry"
                           /note="sequence: tgccacctgacgtctaagaa"
                           /ApEinfo_revcolor=#faac61
                           /ApEinfo_fwdcolor=#faac61
     primer                complement(1535..1589)
                           /label="sfGFP-stop-spacer-Rev"
                           /note="sequence: TACACAGGCGTAGCAGCCTGAGTCGTTAttatttgtacagttcatccataccatg"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
       61 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      361 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
      421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
      481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
      541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
      601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
      661 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
      721 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag ataatacgac
      781 tcactatagg gagatactag aagaaggagA ATAATCTATG gactacaaag acgatgatga
      841 caagatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga
      901 tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa
      961 tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac
     1021 tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa
     1081 gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc
     1141 ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct
     1201 ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca
     1261 taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa
     1321 tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc
     1381 tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca
     1441 ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt
     1501 tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaata
     1561 aTAACGACTC AGGCTGCTAC GCCTGTGTAC TGGAAAACAA AACCAAAACC CAAAAAACAA
     1621 AAAACTGAGC CCATTGGTAT CGTGGAAGGA CTCGATCAAA AAAAAAAAAA AAAAAAAAAA
     1681 AAAAAAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGgtact
     1741 agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1801 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1861 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1921 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1981 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2041 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2101 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2161 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2221 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2281 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2341 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2401 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2461 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2521 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2581 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2641 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2701 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2761 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2821 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2881 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2941 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3001 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tat
//