LOCUS       DT044A_-_pRT30          7122 bp ds-DNA     circular     06-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator            213..259
                           /label="T7 terminator"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     misc_feature          2271..3359
                           /label="LacI"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
     misc_binding          complement(1852..1874)
                           /label="LacO"
                           /ApEinfo_revcolor=#84b0dc
                           /ApEinfo_fwdcolor=#84b0dc
     misc_feature          complement(1777..1794)
                           /label="6x His Tag"
                           /ApEinfo_revcolor=#c7b0e3
                           /ApEinfo_fwdcolor=#c7b0e3
     misc_feature          complement(328..1746)
                           /label="PFO (codon opt)"
                           /ApEinfo_revcolor=#faac61
                           /ApEinfo_fwdcolor=#faac61
     rep_origin            complement(5277..5959)
                           /label="ColE1 origin"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
     misc_feature          complement(325..327)
                           /label="Stop"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     promoter              2211..2270
                           /label="lacIq promoter"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
     misc_feature          complement(1876..1893)
                           /label="T7 promoter"
                           /ApEinfo_revcolor=#75c6a9
                           /ApEinfo_fwdcolor=#75c6a9
     misc_feature          complement(325..1806)
                           /label="ORF"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     CDS                   complement(6057..6716)
                           /label="AmpR"
                           /ApEinfo_revcolor=#ffef86
                           /ApEinfo_fwdcolor=#ffef86
     represor binding site 1852..1872
                           /label="lac represor binding site"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     misc_feature          complement(1750..1767)
                           /label="Thrombin Site"
                           /ApEinfo_revcolor=#ffef86
                           /ApEinfo_fwdcolor=#ffef86
     RBS                   5088..5093
                           /label="RBS"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     MCS                   319..323
                           /label="MCS"
                           /ApEinfo_revcolor=#ffef86
                           /ApEinfo_fwdcolor=#ffef86
     RBS                   complement(3447..3452)
                           /label="RBS"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     primer_bind           complement(1874..1893)
                           /label="T7"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
     primer                213..232
                           /label="FC272"
                           /note="sequence: CTTAACGCCGACTGGCTTCAAAAAACCCCTCAAGACCC"
                           /ApEinfo_revcolor=#f8d3a9
                           /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
      301 cgggctttgt tagcagccgg atccTCAATT ATAGGTAATG CTTGAACCCG GATACAGGGT
      361 GGTCCCCCAA ATCGAAACGT TGATATTATT CGTCAGCGGC ACATCATATT CGCTAATTAC
      421 ATCACGCCAC CATTCCCAGG CCAGCCCCGT TGCTTCACGT GCTTTAATGC GGATGTTGCG
      481 CGCATTGGCT TCCAGCGGGA TAACGGTGCT ATAGTGGGCA GTTTTGTCCT GGTAATTGCC
      541 GTCCCAAGTT TTATGCGTCA GAACTTCGTT ACCTTCTTTA TCGTATGAGA CCTCGTCCCA
      601 CGCCACTTCA AACTGAGCGA CATACGCaCC GGAATGATCC AGGTTGATTT TACCTTTAGA
      661 ATATTCGGTA CTGGTGGTTT CAATGTAATC GGTCTTGTTA TGGACGGCGG CAACGCTGTT
      721 GTCTTTCAGG AAGACAGAGG TATAGCTGAT TGGATAAGCC GGATTCTTCG TactAAAAGT
      781 GGCATTATCT TTGATGACTT TGCGGATTTC GTCAAAATCT TTCGTCACCA CTTTATTATG
      841 TTCCTGTGCA TCCCCTCCCA GCacaacTGC TGTGAAACTG CTATTTTCAT AAATAtctTT
      901 ATACTGtTgA CTAttTTTaa tatctGtaTT TTTGATCAGA GCTTTAAAAG CAGCTTGGAC
      961 ATCTTTCGAG GAGCTAGTGG TTTCCAGTTT AACATAAATG GTGCGACCAT AGGCAACATT
     1021 AGAAACCATC AGCGGCGGAG CTTCGTTGCT AACACCTTTT TGCTTCAGAT CATTAAAAGT
     1081 AACGCTATCA TCGAACAGAT CAGACGGATT TTTCGGCAGA TCGGCACTGA CTGTATAAAA
     1141 GATCTGCTTA TATGCCAGGA TCATTACTTT TTTCTCGTTA TTTGCTACGG CGTTGAAGTC
     1201 AACACCCAGC GAATTTTCCA GCACTTTAGC GTTCACATTC AGGGCCGATG AGATTTGGGA
     1261 TTTACTATAC ACCATGGATT CGGAGTACTG GGTGCGCGCC GGCAGGGTGT GGGTGCTGCT
     1321 ATATTTTTCA TTCCATTTGC TAACCAGTTC ATCAATGGCG CCGCTGACTT TACCATAAGT
     1381 TGGATCATCC ACCTTGATAG AGTTTTCACC TTTCAGACCC GGCAGATCAA TGTTGATATT
     1441 GATCGGTTTA CGTTTTACCA TCAGAATAGT AGGGCGATTT TCGACAAATG CTTTATCAGC
     1501 CAGTTGCAGC GCACCAGGAT ACGTGCGATC ATTAACACTG TCAATAATGG AAATATCAAC
     1561 CGGTGAAGTA GTCAGGCTAC GTTTCTGGCG TTCGACTACA ATGAATTTAT TGCCCGCTTT
     1621 TTTacCCtct TTaggaACAA ACGATTCAAT TTTATCACCA TTGCTGGCCA GGACTTCATT
     1681 GCGGTTATAG CTCAGCGAAC TGATGCCACT ATCAATCGAT TGGTTTTTAT CCGTGATATC
     1741 TTTCATATGG CTGCCGCGCG GCACCAGGCC GCTGCTGTGA TGATGATGAT GATGGCTGCT
     1801 GCCCATGGta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
     1861 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
     1921 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     1981 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     2041 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     2101 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     2161 cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca
     2221 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg
     2281 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     2341 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     2401 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     2461 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     2521 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     2581 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     2641 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     2701 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     2761 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     2821 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     2881 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     2941 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     3001 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     3061 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     3121 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     3181 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     3241 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     3301 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3361 gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg
     3421 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3481 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     3541 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     3601 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     3661 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     3721 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     3781 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     3841 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg
     3901 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     3961 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     4021 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa
     4081 ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
     4141 tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt
     4201 cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
     4261 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
     4321 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
     4381 aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag
     4441 gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac
     4501 cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac
     4561 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt
     4621 tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca
     4681 aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc
     4741 tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg
     4801 accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     4861 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     4921 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     4981 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     5041 actgagagtg caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     5101 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5161 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5221 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5281 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5341 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5401 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5461 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5521 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5581 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5641 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5701 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     5761 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5821 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5881 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5941 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6001 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6061 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6121 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6181 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6241 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6301 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6361 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6421 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6481 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6541 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6601 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6661 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6721 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6781 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6841 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6901 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6961 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7021 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7081 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aa
//