LOCUS       DT049A_-_pr.Tet-BDNF    2722 bp ds-DNA     circular     06-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature          158..165
                           /label="spacer"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
     origin of replication 913..1527
                           /label="rep (pMB1)"
                           /ApEinfo_revcolor=#c7b0e3
                           /ApEinfo_fwdcolor=#c7b0e3
     misc_feature          652..681
                           /label="polyA (30)"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     misc_feature          731..736
                           /label="Scar (AT)"
                           /ApEinfo_revcolor=#f49ac2
                           /ApEinfo_fwdcolor=#f49ac2
     misc_feature          166..192
                           /label="ATG-FLAG-tag"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     misc_feature          193..558
                           /label="BDNF mature"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
     misc_feature          complement(733..738)
                           /label="Scar (AT)"
                           /ApEinfo_revcolor=#f49ac2
                           /ApEinfo_fwdcolor=#f49ac2
     misc_feature          66..85
                           /label="Prefix"
                           /ApEinfo_revcolor=#6ecff6
                           /ApEinfo_fwdcolor=#6ecff6
     terminator            682..729
                           /label="T7 terminator"
                           /ApEinfo_revcolor=#c6c9d1
                           /ApEinfo_fwdcolor=#c6c9d1
     RBS                   complement(2113..2118)
                           /label="RBS"
                           /ApEinfo_revcolor=#f58a5e
                           /ApEinfo_fwdcolor=#f58a5e
     CDS                   complement(2038..2349)
                           /label="AmpR"
                           /ApEinfo_revcolor=#fff79a
                           /ApEinfo_fwdcolor=#fff79a
     misc_feature          87..140
                           /label="pr.Tet"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     terminator stem loop  37..56
                           /label="Terminator stem loop"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
     misc_feature          731..751
                           /label="Suffix"
                           /ApEinfo_revcolor=#f9ad81
                           /ApEinfo_fwdcolor=#f9ad81
     misc_feature          149..157
                           /label="RBS"
                           /ApEinfo_revcolor=#d59687
                           /ApEinfo_fwdcolor=#d59687
     misc_feature          559..617
                           /label="spacer"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     misc_feature          618..647
                           /label="FRET Probes"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     CDS                   complement(1690..2037)
                           /label="AmpR"
                           /ApEinfo_revcolor=#fff79a
                           /ApEinfo_fwdcolor=#fff79a
     primer                2670..2689
                           /label="VF Primer"
                           /note="sequence: tgccacctgacgtctaagaa"
                           /ApEinfo_revcolor=#85dae9
                           /ApEinfo_fwdcolor=#85dae9
     primer                559..592
                           /label="spacer 3'"
                           /note="sequence: CGACTCAGGCTGCTACGCCTGTGTACTGGAAAAC"
                           /ApEinfo_revcolor=#f8d3a9
                           /ApEinfo_fwdcolor=#f8d3a9
     primer                143..168
                           /label="5'UTR Universal fwd"
                           /note="sequence: tactagaagaaggagAATAATCTATG"
                           /ApEinfo_revcolor=#c6c9d1
                           /ApEinfo_fwdcolor=#c6c9d1
     primer                complement(143..168)
                           /label="5'UTR Universal rev"
                           /note="sequence: catAGATTATTctccttcttctagta"
                           /ApEinfo_revcolor=#9eafd2
                           /ApEinfo_fwdcolor=#9eafd2
     primer                complement(564..583)
                           /label="spacer rev"
                           /note="sequence: TACACAGGCGTAGCAGCCTG"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
       61 ttctggaatt cgcggccgct tctagatccc tatcagtgat agagattgac atccctatca
      121 gtgatagaga tactgagcac gatactagaa gaaggagAAT AATCTATGga ctacaaagac
      181 gatgatgaca agATGCACTC CGACCCTGCC CGCCGTGGGG AGCTGAGCGT GTGTGACAGT
      241 ATTAGCGAGT GGGTCACAGC GGCAGATAAA AAGACTGCAG TGGACATGTC TGGCGGGACG
      301 GTCACAGTCC TAGAGAAAGT CCCGGTATCC AAAGGCCAAC TGAAGCAGTA TTTCTACGAG
      361 ACCAAGTGTA ATCCCATGGG TTACACCAAG GAAGGCTGCA GGGGCATAGA CAAAAGGCAC
      421 TGGAACTCGC AATGCCGAAC TACCCAATCG TATGTTCGGG CCCTTACTAT GGATAGCAAA
      481 AAGAGAATTG GCTGGCGATT CATAAGGATA GACACTTCCT GTGTATGTAC ACTGACCATT
      541 AAGAGGGGAA GAtagTAGCG ACTCAGGCTG CTACGCCTGT GTACTGGAAA ACAAAACCAA
      601 AACCCAAAAA ACAAAAAACT GAGCCCATTG GTATCGTGGA AGGACTCGAT CAAAAAAAAA
      661 AAAAAAAAAA AAAAAAAAAA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
      721 GGTTTTTTGg tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc
      781 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      841 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      901 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
      961 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1021 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1081 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1141 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1201 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1261 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1321 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1381 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1441 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1501 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1561 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1621 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1681 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1741 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1801 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1861 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1921 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1981 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2041 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2101 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2161 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2221 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2281 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2341 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2401 gagatccagt tcgatataac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2461 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2521 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2581 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2641 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2701 catgacatta acctataaaa at
//