LOCUS       Exported                8249 bp ds-DNA     circular SYN 30-APR-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   timothyaballo
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 22, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        351..1529
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          582..1520
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     1481..1501
                     /label=Upstrm pEF6 Myc-His Seq
                     /note="color: black; sequence: GCCTCAGACAGTGGTTCAAAG"
     promoter        1546..1564
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     1626..1649
                     /label=Lck Forward (EcoRI)
                     /note="color: black; sequence: 
                     TATATGAATtcgccatgggctgtggctgc"
     CDS             1635..3161
                     /codon_start=1
                     /label=Lck
                     /note="color: #ccffcc"
                     /translation="MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVR
                     DPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLT
                     TGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSF
                     SLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSR
                     PCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMS
                     PDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINK
                     LLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREG
                     AKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYR
                     MVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP"
     primer_bind     2112..2131
                     /label=LGB-S1N1
                     /note="color: black; sequence: GCGGGATCGTTTTCACTGTC"
     primer_bind     2774..2793
                     /label=LGB-S2N1
                     /note="color: black; sequence: TGCAGACTTTGGCCTAGCAC"
     primer_bind     complement(3144..3161)
                     /label=Lck Reverse (HA, STOP, PmeI)
                     /note="color: black; sequence: 
                     ATATGTTTAAACTCAAGCGTAATCTGGAACATCGTATGGGTAaggctgaggctggtact
                     g"
     primer_bind     3153..3191
                     /label=TurboID Forward (BbvCI, Pro, Gly)
                     /note="color: black; sequence: 
                     TATATCCTCAGCCTGGAgctagcaaagacaatactgtgcctctg"
     CDS             3162..3164
                     /codon_start=1
                     /label=Gly
                     /note="color: #ff0000"
                     /translation="G"
     CDS             3165..4133
                     /codon_start=1
                     /label=TurboID
                     /note="color: #99cc00"
                     /translation="ASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEKLQ"
     primer_bind     3516..3535
                     /label=Turbo Seq Forward
                     /note="color: black; sequence: ggaagcagagggaggaaatg"
     primer_bind     complement(3597..3621)
                     /label=TurboID M157T Reverse (Set 1)
                     /note="color: black; sequence: tgaccgggcccaggccgattgctgc; 
                     added: 2019-04-30; 5' phosphorylated"
     primer_bind     complement(3622..3647)
                     /label=TurboID M157T Reverse (Set 2)
                     /note="color: black; sequence: cagcgcttctgccgtgacaattccga; 
                     added: 2019-03-14; 5' phosphorylated"
     primer_bind     3622..3645
                     /label=TurboID M157T Forward (Set 1)
                     /note="color: black; sequence: tcggaattgtcacggcagaagcgc; 
                     added: 2019-04-30; 5' phosphorylated"
     primer_bind     3648..3672
                     /label=TurboID M157T Forward (Set 2)
                     /note="color: black; sequence: cgaaagctgggagcagacaaggtgc; 
                     added: 2019-03-14; 5' phosphorylated"
     primer_bind     complement(3792..3804)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     CDS             4134..4160
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /note="color: #993366"
                     /translation="LPPLERLTL"
     primer_bind     complement(4145..4204)
                     /label=Turbo Reverse (Gly, HA, STOP, PmeI)
                     /note="color: black; sequence: 
                     TATATGTTTAAACTTAAGCGTAATCTGGAACATCGTATGGGTATCCgtccagggtcagg
                     cgctcc"
     CDS             4167..4193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     polyA_signal    4222..4446
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(4351..4371)
                     /label=Dnstrm pEF6 Myc-His Seq
                     /note="color: black; sequence: CACCCCCCAGAATAGAATGAC"
     rep_origin      4492..4920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4934..5263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5114..5249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        5311..5358
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5377..5775
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=Blasticidin R
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5933..6054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6103..6119)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6127..6143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6151..6181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6151 .. 6157 / #ffffff / -10
                        2: 6158 .. 6175 / #ffffff
                        3: 6176 .. 6181 / #ffffff / -35"
     protein_bind    6196..6217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6494..7082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7253..8113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7253 .. 8044 / #ccffcc
                        2: 8045 .. 8113 / #ccffcc / signal sequence
                     Cleavage site after base 8044"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7876..7895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(8114..8218)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg
     1621 tggtggaatt cgccatgggc tgtggctgca gctcacaccc ggaagatgac tggatggaaa
     1681 acatcgatgt gtgtgagaac tgccattatc ccatagtccc actggatggc aagggcacgc
     1741 tgctcatccg aaatggctct gaggtgcggg acccactggt tacctacgaa ggctccaatc
     1801 cgccggcttc cccactgcaa gacaacctgg ttatcgctct gcacagctat gagccctctc
     1861 acgacggaga tctgggcttt gagaaggggg aacagctccg catcctggag cagagcggcg
     1921 agtggtggaa ggcgcagtcc ctgaccacgg gccaggaagg cttcatcccc ttcaattttg
     1981 tggccaaagc gaacagcctg gagcccgaac cctggttctt caagaacctg agccgcaagg
     2041 acgcggagcg gcagctcctg gcgcccggga acactcacgg ctccttcctc atccgggaga
     2101 gcgagagcac cgcgggatcg ttttcactgt cggtccggga cttcgaccag aaccagggag
     2161 aggtggtgaa acattacaag atccgtaatc tggacaacgg tggcttctac atctcccctc
     2221 gaatcacttt tcccggcctg catgaactgg tccgccatta caccaatgct tcagatgggc
     2281 tgtgcacacg gttgagccgc ccctgccaga cccagaagcc ccagaagccg tggtgggagg
     2341 acgagtggga ggttcccagg gagacgctga agctggtgga gcggctgggg gctggacagt
     2401 tcggggaggt gtggatgggg tactacaacg ggcacacgaa ggtggcggtg aagagcctga
     2461 agcagggcag catgtccccg gacgccttcc tggccgaggc caacctcatg aagcagctgc
     2521 aacaccagcg gctggttcgg ctctacgctg tggtcaccca ggagcccatc tacatcatca
     2581 ctgaatacat ggagaatggg agtctagtgg attttctcaa gaccccttca ggcatcaagt
     2641 tgaccatcaa caaactcctg gacatggcag cccaaattgc agaaggcatg gcattcattg
     2701 aagagcggaa ttatattcat cgtgaccttc gggctgccaa cattctggtg tctgacaccc
     2761 tgagctgcaa gattgcagac tttggcctag cacgcctcat tgaggacaac gagtacacag
     2821 ccagggaggg ggccaagttt cccattaagt ggacagcgcc agaagccatt aactacggga
     2881 cattcaccat caagtcagat gtgtggtctt ttgggatcct gctgacggaa attgtcaccc
     2941 acggccgcat cccttaccca gggatgacca acccggaggt gattcagaac ctggagcgag
     3001 gctaccgcat ggtgcgccct gacaactgtc cagaggagct gtaccaactc atgaggctgt
     3061 gctggaagga gcgcccagag gaccggccca cctttgacta cctgcgcagt gtgctggagg
     3121 acttcttcac ggccacagag ggccagtacc agcctcagcc tggagctagc aaagacaata
     3181 ctgtgcctct gaagctgatc gctctcctgg ctaatggcga gttccatagt ggcgaacagc
     3241 tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg
     3301 actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc
     3361 cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc
     3421 ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg
     3481 atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct
     3541 ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag
     3601 caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag
     3661 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag
     3721 gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg
     3781 ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac
     3841 tgcaggaagc agggattaac ctggacagga atactctggc cgctacgctg atccgagagc
     3901 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg ccacggtggg
     3961 agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt
     4021 tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta
     4081 tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagctg cagctgcctc
     4141 ccctggagcg cctgaccctg gacggatacc catacgatgt tccagattac gcttaagttt
     4201 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4261 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4321 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4381 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4441 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     4501 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4561 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4621 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4681 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4741 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4801 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4861 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4921 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4981 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5101 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5161 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5221 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     5281 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     5341 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa
     5401 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     5461 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     5521 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     5581 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     5641 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     5701 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     5761 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     5821 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     5881 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     5941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6061 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6121 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6181 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6241 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6301 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg
     6481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7141 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8221 catttccccg aaaagtgcca cctgacgtc
//
