LOCUS       pCL33-decoder           6684 bp ds-DNA     circular     11-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    782..796
                     /label="tetO1_mut"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3855..4474
                     /label="pBR322 origin "
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    364..716
                     /label="Fus1upstream"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1720..2889
                     /label="MBP-codon opt."
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2949..3298
                     /label="tCyc1 term"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    723..913
                     /label="PminTDH3-tet_mut"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    759..773
                     /label="tetO1_mut"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4637..5497
                     /label="amp marker Marker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             943..1557
                     /label="TetR|CDS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    364..913
                     /label="Pfus1mut"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             1558..1581
                     /label="NLS|CDS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    5900..6550
                     /label="HIS3MX"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3258..3277)
                     /label="FRO4761"
                     /note="sequence: ttccttagtagcgtctgcttACAACAACGGAAGCGATGAT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(3278..3298)
                     /label="FRO4762"
                     /note="sequence: atcatcgcttccgttgttgtaagcagacgctactaaggaaa"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          316..335
                     /label="FRO4337 - FRP1670-lexA-boxes_FW"
                     /note="sequence: tatatttcttttcgcggccg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3278..3298)
                     /label="FRO4762"
                     /note="sequence: atcatcgcttccgttgttgtAAGCAGACGCTACTAAGGAAA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          364..381
                     /label="FRO4763"
                     /note="sequence: gttatctgtaaatacggccgaagctgggtaccACGATGATTCAGTTCGCC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 atatagtacg tagccgtata aattgaattg aatgttcagc ttatagaata gagacacttt
       61 gctgttcaat gcgtcgtcac ttaccatact cactttatta tacgacttta agtataaact
      121 ccgcggttat ggtaaaatta atgatgcaca aacgtccgat cggatgtgct gcaaggcgat
      181 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
      241 tgtaatacga ctcactatag ggcgaattgg agaaacagaa taatatataa acctgtataa
      301 tataaccttg aagactatat ttcttttcgc ggccgcctat agggcgaatt ggagctccct
      361 aggACGATGA TTCAGTTCGC CTTCTATCCT TTGTTTACGT ATTTGTTTAT ATATATAACT
      421 TTATTTTTTT TTATTAATTG GGCTGCAAGA CAATTTTGTT GTCAGTGATG CCTCAATCCT
      481 TCTTTTGCTT CCATATTTAC CATGTGGACC CTTTCAAAAC AGAGTTGTAT CTCTGCAGGA
      541 TGCCCTTTTT GACGTATTGA ATGGCATAAT TGCACTGTCA CTTTTCGCGC TGTCTCATTT
      601 TGGTGCGATG ATGAAACAAA CATGAAACGT CTGTAATTTG AAACAAATAA CGTAATTCTC
      661 GGGATTGGTT TTATTTAAAT GACAATGTAA GAGTGGCTTT GTAAGGTATG TGTTGCtcta
      721 gaaaaggttg aaaccagttc cctgaaatta ttcccctact attcattgaa tagtataaaa
      781 gctattcatt gaatagGTAA TTCTGTAAAT CTATTTCTTA AACTTCTTAA ATTCtacttt
      841 tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa taaacacaca
      901 taaacaaaca aaactagtgg atcccccggg ctgcaggaat tcatgtccag attagataaa
      961 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
     1021 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     1081 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
     1141 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     1201 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     1261 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     1321 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg
     1381 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     1441 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     1501 ttattcggcc ttgaattgat catttgcgga ttagaaaaac aacttaaatg tgaaagtgcc
     1561 cccaagaaaa agagaaaggt ggcagaagcc gccgcaaagg aagcggcagc aaaagaggct
     1621 gcggccaagg aagctgctgc caaggcgcta gaagcagagg cggccgcaaa ggaggcggcc
     1681 gcgaaggaag cggctgcgaa ggaggcagcg gcaaaggcga aaataaagac cggggcgagg
     1741 attcttgcct tgtccgcgtt aacaacaatg atgttttctg caagcgccct agcaaaaatc
     1801 gaggagggaa agttagttat atggatcaat ggtgacaaag gatacaatgg gctagctgaa
     1861 gtcgggaaaa agtttgaaaa ggacacgggg ataaaagtca cggtggagca tccggacaaa
     1921 ctagaagaga aatttcctca ggtagccgca accggggatg ggcccgacat tatattttgg
     1981 gcacatgatc gtttcggggg atacgcccaa agcggtcttc ttgctgaaat aactcctgat
     2041 aaagcattcc aagataagct ttaccctttc acatgggacg ccgttaggta taacggcaaa
     2101 ctgattgctt atcctatagc tgtggaggca ctatctttga tttacaacaa agaccttttg
     2161 ccaaaccctc ccaagacttg ggaagagata ccagccctag ataaagaatt aaaagctaag
     2221 ggaaagtccg cgctaatgtt caatcttcaa gaaccatatt ttacttggcc gttaatagct
     2281 gctgatggtg gatatgcctt taagtacgag aacgggaagt acgacattaa agatgttggc
     2341 gttgacaatg ccggagcgaa ggctggttta actttccttg tggacttaat caaaaataag
     2401 cacatgaacg cggatacaga ctattccatc gccgaagctg ctttcaataa gggagagacc
     2461 gctatgacga tcaacgggcc atgggcatgg tcaaatattg ataccagtaa agtaaattat
     2521 ggagtaaccg ttcttccgac cttcaaagga cagccttcta agcccttcgt gggggtcctt
     2581 tcagcgggca tcaacgcagc aagccctaat aaggaattgg cgaaagagtt tcttgagaac
     2641 tatctactaa ctgacgaggg tttagaggct gtaaacaaag acaagcctct tggcgcagtc
     2701 gcccttaaat cttacgagga agaactagct aaagatccga ggatagccgc taccatggag
     2761 aatgctcaaa aaggagaaat catgccaaac atcccgcaaa tgtctgcatt ttggtatgct
     2821 gtacgtacgg cagtgattaa cgccgcttct ggaagacaga ccgttgacga agcgcttaaa
     2881 gacgcccagt aaacttctaa ataagcgaat ttacgcgttt aaagcttatc gataccgtcg
     2941 acctcgagac aggccccttt tcctttgtct atatcatgta attagttatg tcacgcttac
     3001 attcacgccc tcctcccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
     3061 tctaggtccc tatttatttt ttttaatagt tatgttagta ttaagaacgt tatttatatt
     3121 tcaaattttt cttttttttc tgtacaaacg cgtgtacgca tgtaacatta tactgaaaac
     3181 cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcaaggttc gcagtttaca
     3241 ctctcatcgt cgctctcatc atcgcttccg ttgttgtttt ccttagtagc gtctgcttgg
     3301 tacccagctt cggccgtatt tacagataac gatgtcgtta ttatatatat atatatatat
     3361 tgtcacttgt acccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     3421 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaacagct gacaccgatt
     3481 atttaaagct gcagcatacg atatatatac atgtgtatat atgtatacct atgaatgtca
     3541 gtaagtatgt atacgaacag tatgatactg aagatgacaa ggtaatgcat cattctatac
     3601 gtgtcattct gaacgaggcg cgcctctccc tgcaggcatt aatgaatcgg ccaacgcgcg
     3661 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3721 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3781 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3841 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3901 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3961 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4021 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4081 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4141 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4201 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4261 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4321 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4381 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4441 agaaaaaaag gatctcaaga agatcctttg atcttttgtt taaacctacg gggtctgacg
     4501 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4561 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4621 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4681 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4741 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4801 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4861 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4921 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4981 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5041 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat ggttgtcaga agtaagttgg
     5101 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5161 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5221 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5281 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5341 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5401 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5461 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5521 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5581 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgaaagaa
     5641 cgcggcatta gcaacatttg cgaagcgaac gattttgcga actgctatta attaactctg
     5701 gcgcgcctcc tctagtacac tctatatttt tttatgcctc ggtaatgatt ttcatttttt
     5761 tttttccacc tagcggatga ctcttttttt ttcttagcga ttggcattat cacataatga
     5821 attatacatt atataaagta atgtgatttc ttcgaagaat atactaaaaa atgagcaggc
     5881 aagataaacg aaggcacata tgaggagggc ttttgtagaa agaaatacga acgaaacgaa
     5941 aatcagcgtt gccatcgctt tggacaaagc tcccttacct gaagagtcga attttattga
     6001 tgaacttata acttccaagc atgcaaacca aaagggagaa caagtaatcc aagtagacac
     6061 gggaattgga ttcttggatc acatgtatca tgcactggct aaacatgcag gctggagctt
     6121 acgactttac tcaagaggtg atttaatcat cgatgatcat cacactgcag aagatactgc
     6181 tattgcactt ggtattgcat tcaagcaggc tatgggtaac tttgccggtg ttaaaagatt
     6241 tggacatgct tattgtccac ttgacgaagc tctttccaga agcgtagttg acttgtcggg
     6301 acggccctat gctgttatcg atttgggatt aaagcgtgaa aaggttgggg aattgtcctg
     6361 tgaaatgatc cctcacttac tatattcctt ttcggtagct gctggaatta ctttgcatgt
     6421 tacctgctta tatggtagta atgaccatca tcgtgctgaa agcgctttta aatctctggc
     6481 tgttgccatg cgcgcggcta cttctcttac tggaagttct gaagtcccaa gcacgaaggg
     6541 agtgttgtaa agatgaattg gattatgtca ggaaaagaac gacaattttg catccaaatt
     6601 gtctaaattt tagagttgct tgaaaacaat agaaccttac ttgctttata attacgttaa
     6661 ttagaagcgt tatctcgtga agga
//