LOCUS       pCL38-decoder           6869 bp ds-DNA     circular     11-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2972..3162
                     /label="PminTDH3-tet"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2613..2965
                     /label="Fus1 upstream"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5629..5648
                     /label="T3 prom Promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5..865
                     /label="amp marker Marker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4761..4799
                     /label="HAgeneva tag Tag"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3192..3797
                     /label="LexA CDS"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3813..4754
                     /label="HBD reg Regulatory_Seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5193..5542
                     /label="tCyc1 term Terminator"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1282..2376
                     /label="LEU2 marker Marker"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6092..6711
                     /label="pBR322 origin Rep_Origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3031..3045
                     /label="tetO1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3008..3022
                     /label="tetO1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2468..2484
                     /label="M13 microsynth"
                     /note="sequence: tgtaaaacgacggccag"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
       61 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
      121 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
      181 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
      241 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
      301 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
      361 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
      421 aagcggttag ctccttcggt cctccgatgg ttgtcagaag taagttggcc gcagtgttat
      481 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
      541 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
      601 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
      661 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
      721 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
      781 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
      841 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
      901 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
      961 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgaaagaacg cggcattagc
     1021 aacatttgcg aagcgaacga ttttgcgaac tgctattaat taactctggc gcgccactat
     1081 cgtctatcaa ctaatagtta tattatcaat atattatcat atacggtgtt aagatgatga
     1141 cataagttat gagaagctgt catcgaagtt agaggaagct gaagtgcaag gattgataat
     1201 gtaataggat aatcatatgt ttctattatg aatttcattt ataaagttta tgtacaaata
     1261 tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg cgacagcatc
     1321 accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac ctgcatccaa
     1381 aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca atgacaattt
     1441 caacatcatt gcagcagaca agatagtggc gatagggttg accttattct ttggcaaatc
     1501 tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga
     1561 ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg cttcatcgga
     1621 gatgatgtca ccaaacatgt tgctggtgat tataatacca tttaggtggg ttgggttctt
     1681 aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg taggaaattc
     1741 gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca aaacattagc
     1801 tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga aagcggccat
     1861 tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag cgacaccatc
     1921 accatcgtct tcctttctct taccaaagta aatacctccc actaattctc tgacaacaac
     1981 gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa gagagtcgga
     2041 tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga tttttagtaa
     2101 accttgttca ggtctaacac taccagtacc ccatttagga ccacccacag cacctaacaa
     2161 aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggaa cacctgtagc
     2221 atcgatagca gcaccaccaa ttaaatgatt ttggaaatcg aacttgacat tggaacgaac
     2281 atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc
     2341 acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc cttgaaatat
     2401 atatacgatc ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     2461 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga
     2521 gaaacagaat aatatataaa cctgtataat ataaccttga agactatatt tcttttcgcg
     2581 gccgcctata gggcgaattg gagctcccta ggACGATGAT TCAGTTCGCC TTCTATCCTT
     2641 TGTTTACGTA TTTGTTTATA TATATAACTT TATTTTTTTT TATTAATTGG GCTGCAAGAC
     2701 AATTTTGTTG TCAGTGATGC CTCAATCCTT CTTTTGCTTC CATATTTACC ATGTGGACCC
     2761 TTTCAAAACA GAGTTGTATC TCTGCAGGAT GCCCTTTTTG ACGTATTGAA TGGCATAATT
     2821 GCACTGTCAC TTTTCGCGCT GTCTCATTTT GGTGCGATGA TGAAACAAAC ATGAAACGTC
     2881 TGTAATTTGA AACAAATAAC GTAATTCTCG GGATTGGTTT TATTTAAATG ACAATGTAAG
     2941 AGTGGCTTTG TAAGGTATGT GTTGCtctag aaaaggttga aaccagttcc ctgaaattat
     3001 tcccctatct atcattgata gatataaaag tctatcattg atagagtaat tctgtaaatc
     3061 tatttcttaa acttcttaaa ttctactttt atagttagtc ttttttttag ttttaaaaca
     3121 ccaagaactt agtttcgaat aaacacacat aaacaaacaa aactagtgga tcccccgggc
     3181 tgcaggaatt catgaaagcg ttaacggcca ggcaacaaga ggtgtttgat ctcatccgtg
     3241 atcacatcag ccagacaggt atgccgccga cgcgtgcgga aatcgcgcag cgtttggggt
     3301 tccgttcccc aaacgcggct gaagaacatc tgaaggcgct ggcacgcaaa ggcgttattg
     3361 aaattgtttc cggcgcatca cgcgggattc gtctgttgca ggaagaggaa gaagggttgc
     3421 cgctggtagg tcgtgtggct gccggtgaac cacttctggc gcaacagcat attgaaggtc
     3481 attatcaggt cgatccttcc ttattcaagc cgaatgctga tttcctgctg cgcgtcagcg
     3541 ggatgtcgat gaaagacatc ggcattatgg atggtgactt gctggcagtg cataaaactc
     3601 aggatgtacg taacggtcag gtcgttgtcg cacgtattga tgacgaggtt accgttaagc
     3661 gcctgaaaaa acagggcaat aaagtcgaac tgttgccaga aaatagcgag tttaaaccaa
     3721 ttgtcgtaga tcttcgtcag cagagcttca ccattgaagg gctggcggtt ggggttattc
     3781 gcaacggcga ctggctgggt tctaaggata tctctgctgg agacatgaga gctgccaacc
     3841 tttggccaag cccgctcatg atcaaacgct ctaagaagaa cagcctggcc ttgtccctga
     3901 cggccgacca gatggtcagt gccttgttgg atgctgagcc ccccatactc tattccgagt
     3961 atgatcctac cagacccttc agtgaagctt cgatgatggg cttactgacc aacctggcag
     4021 acagggagct ggttcacatg atcaactggg cgaagagggt gccaggcttt gtggatttga
     4081 ccctccatga tcaggtccac cttctagaat gtgcctggtt agagatcctg atgattggtc
     4141 tcgtctggcg ctccatggag cacccagtga agctactgtt tgctcctaac ttgctcttgg
     4201 acaggaacca gggaaaatgt gtagagggca tggtggagat cttcgacatg ctgctggcta
     4261 catcatctcg gttccgcatg atgaatctgc agggagagga gtttgtgtgc ctcaaatcta
     4321 ttattttgct taattctgga gtgtacacat ttctgtccag caccctgaag tctctggaag
     4381 agaaggacca tatccaccga gtcctggaca agatcacaga cactttgatc cacctgatgg
     4441 ccaaggcagg cctgaccctg cagcagcagc accagcggct ggcccagctc ctcctcatcc
     4501 tctcccacat caggcacatg agtaacaaag gcatggagca tctgtacagc atgaagtgca
     4561 agaacgtggt gcccctctat gacctgctgc tggagatgct ggacgcccac cgcctacatg
     4621 cgcccactag ccgtggaggg gcatccgtgg aggagacgga ccaaagccac ttggccactg
     4681 cgggctctac ttcatcgcat tccttgcaaa agtattacat cacgggggag gcagagggtt
     4741 tccctgccac agtcgatatc atgcaggatc tgccgggcaa cgataacagc accgcgggcg
     4801 aatttccagg tattactttg agaatccaag aaactgatat gttgtataaa ggtgatactt
     4861 tgtatttgga ttggttggaa gatggtattg ctgaattggt tttcgatgct ccaggttctg
     4921 ttaataaatt ggatactgct gttgcttctt tgggtgaagc tattggtgtt ttggaacaac
     4981 aatctgattt gatttgggaa actttgactg ttaaagatgc taaagttaat ttcgattctg
     5041 gtttggaaaa attcgaagaa gctattccat ctgctgatga tttcgatcca gttgctgaaa
     5101 gaagatcctc tggtgaattt agagctgaaa gacattctgg tggtactgat ttgtgtttca
     5161 agctttaaac gcgtttaaag cttatcctcg agacaggccc cttttccttt gtctatatca
     5221 tgtaattagt tatgtcacgc ttacattcac gccctcctcc cacatccgct ctaaccgaaa
     5281 aggaaggagt tagacaacct gaagtctagg tccctattta ttttttttaa tagttatgtt
     5341 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta
     5401 cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt
     5461 aatttgcaag gttcgcagtt tacactctca tcgtcgctct catcatcgct tccgttgttg
     5521 ttttccttag tagcgtctgc ttggtaccca gcttcggccg tatttacaga taacgatgtc
     5581 gttattatat atatatatat atattgtcac ttgtacccag cttttgttcc ctttagtgag
     5641 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5701 cgctcacaac agctggaaac atataaaacg gaatgaggaa taatcgtaat attagtatgt
     5761 agaaatatag attccatttt gaggattcct atatccttga ggagaacttc tagtatattc
     5821 tgtataccta atattatagc ctttatcaac aatggcgcgc ctctccctgc aggcattaat
     5881 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5941 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6001 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6061 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6121 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6181 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6241 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6301 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6361 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6421 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6481 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6541 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6601 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6661 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttgtttaa
     6721 acctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6841 ctaaagtata tatgagtaaa cttggtctg
//