LOCUS       pCL66-decoder           6874 bp ds-DNA     circular     11-AUG-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             4689..4712
                     /label="NLS|CDS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3890..3904
                     /label="tetO1_mut"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             4074..4688
                     /label="TetR|CDS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    887..1747
                     /label="amp marker Marker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_binding    6597..6602
                     /label="misc_binding"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3468..3487
                     /label="FRP1736-SacI_lexA-box_FW_overhang"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3495..3847
                     /label="Fus1upstream"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_binding    6810..6816
                     /label="misc_binding"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(6062..6078)
                     /label="pBluescriptKS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3854..4044
                     /label="PminTDH3-tet_mut"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6516..6535
                     /label="T3 prom Promoter"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3410..3459
                     /label="Deg1 insulator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(6061..6077)
                     /label="KS primer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    1438..1443
                     /label="misc_binding"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    105..724
                     /label="pBR322 origin Rep_Origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    6753..6760
                     /label="misc_binding"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3374..3392
                     /label="T7 prom Promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4851..6020
                     /label="MBP-codon opt."
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2164..3258)
                     /label="LEU2 marker Marker"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3913..3927
                     /label="tetO1_mut"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6080..6429
                     /label="tCyc1 term|Unknown"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(4229..4248)
                     /label="FRO4817 qPCR TetR REV"
                     /note="sequence: tctcaatggctaaggcgtcg"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          3495..3511
                     /label="FRO4792 Fus1_FW_tAct1_overhang"
                     /note="sequence: aatgtttatccattggaccgtgtaACGATGATTCAGTTCGC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          3447..3466
                     /label="FRO4337 - FRP1670-lexA-boxes_FW"
                     /note="sequence: tatatttcttttcgcggccg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4057..4073)
                     /label="FRO4342 promoter in FRP20x REV"
                     /note="sequence: gaattcctgcagcccgg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2797..2816)
                     /label="FRO2930"
                     /note="sequence: aggaagacgatggtgatggt"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2703..2723
                     /label="FRO2931"
                     /note="sequence: gcaatggtggctcatgttgta"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          4163..4182
                     /label="FRO4816 qPCR TetR FW"
                     /note="sequence: cgcccagaagctaggtgtag"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
       61 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      121 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      181 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      241 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      301 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      361 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      421 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      481 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      541 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      601 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      661 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      721 atcttttgtt taaacctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      781 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      841 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      901 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      961 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1021 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1081 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1141 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1201 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1261 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1321 gtcctccgat ggttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1381 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1441 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1501 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1561 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1621 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1681 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1741 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1861 cccgaaaagt gccacctgac gtcgaaagaa cgcggcatta gcaacatttg cgaagcgaac
     1921 gattttgcga actgctatta attaactctg gcgcgccact atcgtctatc aactaatagt
     1981 tatattatca atatattatc atatacggtg ttaagatgat gacataagtt atgagaagct
     2041 gtcatcgaag ttagaggaag ctgaagtgca aggattgata atgtaatagg ataatcatat
     2101 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
     2161 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
     2221 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
     2281 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
     2341 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca
     2401 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
     2461 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatgt caccaaacat
     2521 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
     2581 agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc
     2641 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
     2701 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
     2761 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
     2821 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
     2881 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
     2941 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
     3001 actaccagta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt
     3061 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc
     3121 aattaaatga ttttggaaat cgaacttgac attggaacga acatcagaaa tagctttaag
     3181 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
     3241 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatacga tcggatgtgc
     3301 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     3361 ggccagtgaa ttgtaatacg actcactata gggcgaattg gagaaacaga ataatatata
     3421 aacctgtata atataacctt gaagactata tttcttttcg cggccgccta tagggcgaat
     3481 tggagctccc taggACGATG ATTCAGTTCG CCTTCTATCC TTTGTTTACG TATTTGTTTA
     3541 TATATATAAC TTTATTTTTT TTTATTAATT GGGCTGCAAG ACAATTTTGT TGTCAGTGAT
     3601 GCCTCAATCC TTCTTTTGCT TCCATATTTA CCATGTGGAC CCTTTCAAAA CAGAGTTGTA
     3661 TCTCTGCAGG ATGCCCTTTT TGACGTATTG AATGGCATAA TTGCACTGTC ACTTTTCGCG
     3721 CTGTCTCATT TTGGTGCGAT GATGAAACAA ACATGAAACG TCTGTAATTT GAAACAAATA
     3781 ACGTAATTCT CGGGATTGGT TTTATTTAAA TGACAATGTA AGAGTGGCTT TGTAAGGTAT
     3841 GTGTTGCtct agaaaaggtt gaaaccagtt ccctgaaatt attcccctac tattcattga
     3901 atagtataaa agctattcat tgaatagGTA ATTCTGTAAA TCTATTTCTT AAACTTCTTA
     3961 AATTCtactt ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcga
     4021 ataaacacac ataaacaaac aaaactagtg gatcccccgg gctgcaggaa ttcatgtcca
     4081 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
     4141 aaggtttaac aacccgtaaa ctCGCCCAGA AGCTAGGTGT AGAGCAGCCT ACATTGTATT
     4201 GGCATGTAAA AAATAAGCGG GCTTTGCTCG ACGCCTTAGC CATTGAGAtg ttagataggc
     4261 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     4321 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     4381 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     4441 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
     4501 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     4561 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     4621 agccagcctt cttattcggc cttgaattga tcatttgcgg attagaaaaa caacttaaat
     4681 gtgaaagtgc ccccaagaaa aagagaaagg tggcagaagc cgccgcaaag gaagcggcag
     4741 caaaagaggc tgcggccaag gaagctgctg ccaaggcgct agaagcagag gcggccgcaa
     4801 aggaggcggc cgcgaaggaa gcggctgcga aggaggcagc ggcaaaggcg aaaataaaga
     4861 ccggggcgag gattcttgcc ttgtccgcgt taacaacaat gatgttttct gcaagcgccc
     4921 tagcaaaaat cgaggaggga aagttagtta tatggatcaa tggtgacaaa ggatacaatg
     4981 ggctagctga agtcgggaaa aagtttgaaa aggacacggg gataaaagtc acggtggagc
     5041 atccggacaa actagaagag aaatttcctc aggtagccgc aaccggggat gggcccgaca
     5101 ttatattttg ggcacatgat cgtttcgggg gatacgccca aagcggtctt cttgctgaaa
     5161 taactcctga taaagcattc caagataagc tttacccttt cacatgggac gccgttaggt
     5221 ataacggcaa actgattgct tatcctatag ctgtggaggc actatctttg atttacaaca
     5281 aagacctttt gccaaaccct cccaagactt gggaagagat accagcccta gataaagaat
     5341 taaaagctaa gggaaagtcc gcgctaatgt tcaatcttca agaaccatat tttacttggc
     5401 cgttaatagc tgctgatggt ggatatgcct ttaagtacga gaacgggaag tacgacatta
     5461 aagatgttgg cgttgacaat gccggagcga aggctggttt aactttcctt gtggacttaa
     5521 tcaaaaataa gcacatgaac gcggatacag actattccat cgccgaagct gctttcaata
     5581 agggagagac cgctatgacg atcaacgggc catgggcatg gtcaaatatt gataccagta
     5641 aagtaaatta tggagtaacc gttcttccga ccttcaaagg acagccttct aagcccttcg
     5701 tgggggtcct ttcagcgggc atcaacgcag caagccctaa taaggaattg gcgaaagagt
     5761 ttcttgagaa ctatctacta actgacgagg gtttagaggc tgtaaacaaa gacaagcctc
     5821 ttggcgcagt cgcccttaaa tcttacgagg aagaactagc taaagatccg aggatagccg
     5881 ctaccatgga gaatgctcaa aaaggagaaa tcatgccaaa catcccgcaa atgtctgcat
     5941 tttggtatgc tgtacgtacg gcagtgatta acgccgcttc tggaagacag accgttgacg
     6001 aagcgcttaa agacgcccag taaacttcta aataagcgaa tttacgcgtt taaagcttat
     6061 cgataccgtc gacctcgaga caggcccctt ttcctttgtc tatatcatgt aattagttat
     6121 gtcacgctta cattcacgcc ctcctcccac atccgctcta accgaaaagg aaggagttag
     6181 acaacctgaa gtctaggtcc ctatttattt tttttaatag ttatgttagt attaagaacg
     6241 ttatttatat ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc atgtaacatt
     6301 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaaggtt
     6361 cgcagtttac actctcatcg tcgctctcat catcgcttcc gttgttgttt tccttagtag
     6421 cgtctgcttg gtacccagct tcggccgtat ttacagataa cgatgtcgtt attatatata
     6481 tatatatata ttgtcacttg tacccagctt ttgttccctt tagtgagggt taatttcgag
     6541 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaacagc
     6601 tggaaacata taaaacggaa tgaggaataa tcgtaatatt agtatgtaga aatatagatt
     6661 ccattttgag gattcctata tccttgagga gaacttctag tatattctgt atacctaata
     6721 ttatagcctt tatcaacaat ggcgcgcctc tccctgcagg cattaatgaa tcggccaacg
     6781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6841 gcgctcggtc gttcggctgc ggcgagcggt atca
//