LOCUS Exported 5180 bp ds-DNA circular SYN 10-AUG-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pVC 144 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5180) AUTHORS Andrew TITLE Direct Submission JOURNAL Exported Monday, Aug 10, 2020 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5180 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /note="AAV2 ITR" /note="inverted terminal repeat of adeno-associated virus serotype 2" misc_feature 156..559 /note="LAP2" regulatory 566..575 /regulatory_class="other" /note="Kozak sequence" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 572..1282 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="mCherry" /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 1307..1895 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(1778..1789) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" polyA_signal 1927..2403 /note="hGH poly(A) signal" /note="human growth hormone polyadenylation signal" repeat_region 2443..2583 /note="AAV2 ITR" /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 2658..3113 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3395..3499 /gene="bla" /note="AmpR promoter" CDS 3500..4360 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4531..5119 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtatccc cggtccgcgc tccgcccacc 181 catcacagca gccgcggacg ctgcgcgccg gagcggtcca tctcgccagc cagccaacca 241 gccgagccgc ccagccgacc cgagagcccc gagagccaga ctccctcagc catagaagac 301 accgggcggg agagacggac tgaaaaaata tatctttttt tattttgtct gggcctggag 361 acccgcagca ggagcggagg tgggtgcggg gccgggagcc ggagcaggac cgggaacagg 421 aacaggaaca ggaacaggaa caggaacagg agtggggccg ggagcaggag caggagcggg 481 agccgaagtg ggggcaggag cggcggcggc cgcagcagca acagggtcgc cccagtccgc 541 ggcgaggaag agggagctcg gatccgccac catggtgagc aagggcgagg aggataacat 601 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 661 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 721 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 781 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 841 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 901 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg 961 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 1021 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 1081 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 1141 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 1201 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 1261 catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataatc aacctctgga 1321 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 1381 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 1441 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 1501 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 1561 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 1621 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 1681 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac 1741 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 1801 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 1861 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag 1921 atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 1981 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 2041 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 2101 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 2161 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 2221 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 2281 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 2341 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 2401 cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg 2461 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg 2521 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc 2581 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac 2641 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 2701 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 2761 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 2821 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga 2881 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 2941 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg 3001 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 3061 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg 3121 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac 3181 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 3241 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 3301 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 3361 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 3421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 3481 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 3541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 3601 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 3661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 3721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 3781 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 3841 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 3901 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 3961 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 4021 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 4081 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 4141 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 4201 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 4261 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 4321 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 4381 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 4441 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 4501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 4561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 4621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 4681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 4741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 4801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 4861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 4921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 4981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 5041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 5101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 5161 ctggcctttt gctcacatgt //