LOCUS Exported 5277 bp ds-DNA circular SYN 10-AUG-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pVC 143 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5277) AUTHORS Andrew TITLE Direct Submission JOURNAL Exported Monday, Aug 10, 2020 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5277 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /note="AAV2 ITR" /note="inverted terminal repeat of adeno-associated virus serotype 2" misc_feature 159..656 /note="LAP1" regulatory 663..672 /regulatory_class="other" /note="Kozak sequence" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 669..1379 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="mCherry" /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 1404..1992 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(1875..1886) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" polyA_signal 2024..2500 /note="hGH poly(A) signal" /note="human growth hormone polyadenylation signal" repeat_region 2540..2680 /note="AAV2 ITR" /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 2755..3210 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3492..3596 /gene="bla" /note="AmpR promoter" CDS 3597..4457 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4628..5216 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtcccgg gggagtcggg ggggagtcgg 181 gggggagtcg gggggtccca gttcggcgcg gtctccacag cgcacaaagt ccggctgcag 241 taaatttact gcgggtgcag ttaccctggc ggccacaccg gcccgatggc gctgcagtaa 301 ctctactgca gctacagttt actacagttg cagtaccgcg cgccgccgcc aaatactaca 361 gtagatttcc tgtggccgcc gcgtactgca gtttaccgcg gctgcagtaa actgcagtat 421 cgcgcggtaa attgcagtct ggcagccgcg cgttactgca attagcggtg gctcccgaca 481 ctctggccaa ttggtgctaa tgggccgtga tggtccatgt gggggtgatg taaccgccgg 541 gcccggttgg gcactcagat ggtggccggg cgccaggcca aagtgctgtc tgagtgccac 601 tttatgactt tgtttttctc aaacaacatc aattatggat gcacatcgtg tatataggat 661 ccgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 721 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 781 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 841 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 901 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 961 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 1021 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 1081 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 1141 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 1201 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 1261 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 1321 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag 1381 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 1441 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 1501 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 1561 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 1621 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 1681 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 1741 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 1801 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 1861 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 1921 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 1981 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga 2041 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt 2101 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg 2161 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg 2221 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc 2281 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg 2341 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc 2401 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct 2461 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac 2521 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc 2581 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 2641 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt 2701 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 2761 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 2821 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 2881 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 2941 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 3001 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 3061 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 3121 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 3181 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 3241 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 3301 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 3361 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 3421 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 3481 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 3541 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 3601 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 3661 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 3721 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 3781 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 3841 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 3901 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 3961 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 4021 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 4081 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 4141 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 4201 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 4261 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 4321 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 4381 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 4441 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 4501 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 4561 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 4621 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 4681 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 4741 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 4801 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 4861 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 4921 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 4981 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 5041 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 5101 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 5161 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 5221 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt //