LOCUS       Exported                6220 bp ds-DNA     circular SYN 13-AUG-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2020 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    34..223
                     /label=ITR
                     /note="color: #66cc99; direction: RIGHT"
     promoter        487..764
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1186..2670
                     /codon_start=1
                     /label=NIR-GECO2G
                     /note="color: #ff00ff"
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNFVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKATNIAPALVGALHRITSSSSLMGLCDETATIFREITGFD
                     RVMVMRLGALDDLTEEQIAEIKEAFSLFDKDGDGTITTKELGTVFRSLGQNPTEAELQD
                     MINEVDADGDGIFDFPEFLTMMARKMNDSDSEEEIRGAFRVFDKDGNGYIGAAELRHVM
                     TDLGEKLTDEEVDEMIRVADNDGDGQVNYEEFVQMMTAKGGGGSVDSSRRKWNKAGHAV
                     RAIGRLGSRRHDLLSECRRADLEAFLGNRYPASTIPQIARRLYELNRVRLLVDVNYTPV
                     PLEPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIVCHH
                     YEPRYVPSHIRAAGEALAEACANRIATLESFAQSQSK"
     misc_feature    2698..3286
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     polyA_signal    3329..3450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    complement(3553..3742)
                     /label=ITR
                     /note="color: #66cc99; direction: LEFT"
     rep_origin      3918..4802
                     /label=pUC Origin
                     /note="pUC Origin"
                     /note="color: #ffff00"
     CDS             complement(4737..5597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4737 .. 5528 / #ccffcc
                        2: 5529 .. 5597 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5598..5702)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(5728..6183)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
       61 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
      121 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
      181 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcgtaggtt aattaaccat
      241 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      301 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      361 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      421 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      481 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      541 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      601 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      661 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      721 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
      781 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      841 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
      901 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      961 cgggagggcc ctttgtgcgg ggggagcggc tcggggctgt ccgcgggggg acggctgcct
     1021 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1081 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1141 tattgtgctg tctcatcatt ttggcaaaga attggatccg ccaccatgtc ggtaccgctg
     1201 actacctcag cattcggcca cgcgtttctg gctaactgtg aacgtgagca gatccacctg
     1261 gcgggctcca ttcagccgca cggtatcctg ctggctgtta aagagcctga caacgtggtg
     1321 atccaggctt ctattaacgc tgcggagttc ctgaacacca actttgttgt tggccgtccg
     1381 ctgcgtgacc tgggcggcga tctgcctttg cagatcctgc cgcacctgaa cggcccgctg
     1441 cacctggctc cgatgaccct gcgttgtact gtgggttctc cgccgcgtcg tgtggactgt
     1501 accattcatc gaccgtctaa cggcggcctg atcgtagaac tggaaccagc aaccaaggcc
     1561 actaacattg cgccggctct ggtcggtgcg cttcatcgta tcacttcttc atcctccctg
     1621 atgggcctgt gtgacgaaac cgcgactatt ttccgtgaga ttactggttt cgaccgtgtg
     1681 atggtaatgc gtctcggcgc gcttgacgat ctgactgaag agcagatcgc agagattaaa
     1741 gaggctttct ccctatttga caaggacggg gacgggacga taacaaccaa ggagctgggg
     1801 acggtgttcc ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat
     1861 gaagtagatg ccgatggcga cggcatattc gacttccctg agttcctgac gatgatggca
     1921 aggaaaatga atgactcaga cagtgaagag gaaattagag gagcgttccg cgtgtttgat
     1981 aaggacggca atggctacat cggcgcagca gagcttcgcc acgtgatgac agaccttggt
     2041 gagaagttaa ctgatgagga ggttgatgaa atgatcaggg tagcagacaa cgatggggat
     2101 ggtcaggtaa actacgaaga gtttgtacaa atgatgacag cgaagggtgg cggaggttct
     2161 gtagattcat cacgtcgtaa gtggaataag gcaggtcacg cagtcagagc tataggtcgg
     2221 ctgggctcgc gtaggcatga tttgctgtcc gaatgtcgtc gtgcggacct ggaagcgttc
     2281 ctaggtaacc gctacccggc gtctactatt ccgcagatcg ctcgtcgcct gtacgaactc
     2341 aaccgtgttc gcctgctggt agatgtgaac tatactccgg ttccgctaga gccgcgcatc
     2401 agcccgctga acggtcgtga tctggatatg tccctgtctt gcctgcgctc tatgtccccg
     2461 atccaccaga aatacatgca ggacatgggc gttggcgcaa ccctggtttg ctctctgatg
     2521 gtgtctggtc gtctgtgggg tctgatcgtt tgccaccact acgaaccgcg ctacgttccg
     2581 tcccacattc gcgctgctgg cgaagcgctg gcggaagcat gtgcgaaccg catcgcgacg
     2641 ctggagagct ttgcacagtc tcagtccaaa tgagaattcg atatcaagct tatcgataat
     2701 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2761 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2821 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2881 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2941 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3001 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3061 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3121 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3181 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3241 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     3301 cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     3361 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3421 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3481 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     3541 aaaatcgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat
     3601 cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3661 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3721 agtgagcgag cgagcgcgca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3781 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3841 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3901 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3961 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4021 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4081 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4141 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4201 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4261 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4321 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4381 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4441 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4501 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4561 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4621 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4681 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4741 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4801 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4861 gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag
     4921 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4981 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5041 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5101 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5161 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5221 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5281 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5341 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5401 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5461 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5521 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5581 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5641 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5701 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     5761 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5821 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5881 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5941 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     6001 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6061 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6121 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6181 cgtcccattc gccattcagg ctgcgcaact gttgggaagg
//
