LOCUS       Exported                8508 bp ds-DNA     circular SYN 13-SEP-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2020 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    54..2717
                     /label=tag-168 promoter
                     /note="color: #ff8040"
     primer_bind     2796..2816
                     /label=pSF11-Fw-Seq
                     /note="color: black; sequence: cattttcaggaggacccttgg"
     misc_feature    2834..4318
                     /label=wNIR-GECO2
                     /note="color: #ff00ff"
     CDS             4328..4339
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4339"
                     /translation="IEGR"
     CDS             4331..4384
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 4381"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(5293..5309)
                     /label=pSF11-Rv-Seq
                     /note="color: black; sequence: gaagtcagaggcacggg"
     promoter        6387..6491
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6492..7352
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6492 .. 6560 / #ccffcc / signal sequence
                        2: 6561 .. 7352 / #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             6690..7349
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      7447..8129
                     /label=ColE1 origin
                     /note="color: #808080"
     rep_origin      7523..8111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    8399..8420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        8435..8465
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 8435 .. 8440 / #ffffff / -35
                        2: 8441 .. 8458 / #ffffff
                        3: 8459 .. 8465 / #ffffff / -10"
     misc_binding    8469..8491
                     /label=LacO
                     /note="color: #6495ed"
     protein_bind    8473..8489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     join(8497..8508,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
       61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
      121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
      181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
      241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
      301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
      361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
      421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
      481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
      541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
      601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
      661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
      721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
      781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
      841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
      901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
      961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
     1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
     1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
     1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
     1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
     1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
     1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttta atttcgaatt
     1381 tttttaaact tgttgtaact tttttccctg tcggcgccaa ttaacaaaat acgtagaatt
     1441 ttgcgcttat tgttttgtta gttaacaaat ttttgacaac tttcttatca gctgagattt
     1501 aggtgtttca gttcgcaatc tgcgtctctc cttgctctcc tctattttaa aacccccata
     1561 actttgttct tgtgagagtt atcaaaaaat tgtcaactaa caaaatatag agcacaaaaa
     1621 tatgcacatt ttgtatgttg acaatttttt gatacctttt ttagttttcg agatatctta
     1681 gttttagttt agctgtacct ttttctggca aaaaacttcc aaatttcggc aatttttcgg
     1741 caaatttccc ggcaaatttc gccaagtaca tcgggctctc agacaagtgg tctgtgagtg
     1801 gaagtcgata aaagtagttt acgtgctctc cgtccccccg cttcttctcc gtctctgctt
     1861 ctatttctcc attgactagt cccactactt tttacggggc ggagggctct cttgcactcc
     1921 agcttcttct ctttttgtgc tcttttttgc ccgtatgtgg gtgctcattt atttttcttt
     1981 ggttttaaaa taaattcggt ttattaggct actcgtaaaa atgaagaaaa aacttcaaat
     2041 tcaaacgaca ttaaaaaaaa atttaataaa aattttttga attgctcaaa agccccacct
     2101 atatatatat atagctccgc ctacttctcg cacagacccc gcccactttt tgactccgcc
     2161 cccttattgg caaaagttct tgaaaaaaat aaaaataaaa attcaaattg aaacaatttt
     2221 tttttaatga agtagttttt ttctaaattg atgaaaattt ctaaacagtt tacaagcacc
     2281 gcccactttt tgactccgcc cctttgttgg aaaaattctt gtaaaaaaaa ataaaaaata
     2341 aaagcttctc tttcaaacga tttttagttg gtttttgtat gaaaagtttt tttcctaaaa
     2401 tagaaatgtc tgaataattt gcaagctccg cccttttctt acccagactc cgccccttta
     2461 ttgactaatt tttttttgtc catttacctg aagttcaaaa aaaaaattta cagtttcata
     2521 gagtaataat ttttcaaaaa tctctttttc caatgtttaa tgtgtcatta taaacccgcc
     2581 cagttttaag ctccgcccat ttaggccccg cccatttatg acccaaatat ttttttctct
     2641 attcacccgt ggatttccct ccaacaagtt accccaaaaa taaaaataaa aattcctgca
     2701 ggcgcccacc gggatccggc gcgcctctag aggatccccg ggattggcca aaggacccaa
     2761 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
     2821 cgtcgacggt accatgtcgg tgccattaac cacctctgcc ttcggacatg ctttcctcgc
     2881 taactgcgag cgtgagcaaa tccacttagc aggctcaatt caaccgcatg gcatactttt
     2941 ggcggttaaa gaacctgaca atgttgtgat ccaagctagt ataaatgcag ccgagttttt
     3001 aaacaccaat ttcgtagtgg gccgacctct tagagatttg ggtggagacc tcccgttaca
     3061 aatactcccg catttgaacg gtccgcttca tctcgcgcca atgactttgc gatgcactgt
     3121 gggttcccca ccaagacgtg tagattgcac aatacatcgt ccgtcaaatg gaggacttat
     3181 tgtagaattg gagccggcaa cgaaagcgac gaatatcgct ccggccttag ttggagcgct
     3241 ccacagaata acgtcatcgt cctccttaat gggtttgtgc gacgaaacag ccaccatatt
     3301 tcgtgagatt acgggcttcg accgagtaat ggtgatgcga ctcggcgccc tcgatgacct
     3361 taccgaagaa caaatcgcgg agattaaaga ggctttttcg ctttttgaca aggatggaga
     3421 cggtaccatt accaccaagg agcttggaac cgtcttccgt agtttgggcc agaatccgac
     3481 ggaggccgaa ttgcaagaca tgatcaacga ggtagacgca gacggtgatg gcatctttga
     3541 cttcccggag tttcttacaa tgatggcgcg taaaatgaac gatacagact cggaggaaga
     3601 gatccgaggt gcatttcgtg tattcgataa agacggaaac ggctacatag gcgccgcaga
     3661 attacgacac gtaatgacag accttggtga gaagcttaca gacgaagagg tggacgagat
     3721 gattcgagtc gccgataatg acggcgatgg tcaggtaaac tatgaggagt ttgtgcaaat
     3781 gatgaccgca aaaggtggcg gcggatctgt tgactcaagt agacgtaaat ggaacaaggc
     3841 gggacacgcc gttcgtgcca tcggccgttt gagttccaga agacatgacc ttttgtcaga
     3901 atgtcgtcga gcagacctgg aagcatttct cggaaaccga tacccggctt ccacaatccc
     3961 tcaaattgca agaagactgt acgagcttaa cagagtgcgt ttgctggtgg atgtgaatta
     4021 taccccagta ccacttgagc cacgtatatc ccctttaaac ggtcgagacc tcgacatgtc
     4081 attatcgtgt ctgcgaagta tgtctcctat tcaccagaag tatatgcagg atatgggcgt
     4141 gggtgctacg cttgtttgtt cgttgatggt aagtggcaga ttgtggggac ttattgtatg
     4201 tcaccactac gagccaagat atgtcccatc gcacatccgt gccgccggcg aggcgctcgc
     4261 tgaagcgtgt gctaatcgta tcgccacact tgagtcattc gcacagtcgc aatcaaaggg
     4321 ttacgatatt gaaggaagag gaagtttatt gacctgtgga gacgtggaag aaaatccagg
     4381 accgatggaa cgtcctcaac ctgattccat gcctcaggat ttatccgagg ccttgaaaga
     4441 agccacaaaa gaagtgcata cccaagcaga aaacgcagaa ttcatgcgaa acttccaaaa
     4501 gggtcaggta acccgagacg gattcaagct cgtcatggct tccctgtacc atatatacgt
     4561 ggccctcgag gaagaaattg agagaaacaa agagtcgcca gtttttgcgc cagtgtactt
     4621 cccggaagag ctgcacagaa aagctgcatt ggaacaggac ctggcatttt ggtatggacc
     4681 aagatggcag gaagtcattc cgtacacacc tgcaatgcaa agatatgtga agagattgca
     4741 tgaggtcggc cgaacagagc ctgagctgtt agtagcgcat gcctataccc gttatcttgg
     4801 cgacttgtca ggtggacaag tattgaagaa aattgcccaa aaagctctgg atttaccaag
     4861 ttctggagaa ggattagcat tttttacatt tccgaatata gcaagtgcca ctaaattcaa
     4921 gcaactctat cgtagtagaa tgaactctct cgagatgaca ccggccgtca gacagcgagt
     4981 catagaagaa gcgaagacgg cctttttgct gaatatacaa ttgtttgagg agctccagga
     5041 gctgctgact cacgatacca aagaccagtc accgagtcga gcgccaggct tgagacaacg
     5101 tgcatctaac aaggtgcagg attccgcgcc tgtcgagaca cctcgtggca agccgccttt
     5161 aaacacacga tcacaagcac cactgttgcg ttgggtattg acgttgtcgt tcctcgtggc
     5221 aactgtggcc gttggtctgt acgccatgta agagctccgc atcggccgct gtcatcagat
     5281 cgccatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     5341 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaaa cttcttctta
     5401 atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa
     5461 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     5521 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttttactt
     5581 ctgataaatt ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     5641 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     5701 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     5761 aagtttatga aattaatttt cctgcttttg ctttttgggg gtttccccta ttgtttgtca
     5821 agagtttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     5881 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     5941 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     6001 taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc
     6061 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     6121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     6181 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     6241 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaattaagg
     6301 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6361 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6421 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6481 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6541 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6601 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6661 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6721 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6781 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6841 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6901 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6961 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7021 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7081 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7141 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7201 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7261 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7321 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7381 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7441 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7501 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7561 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7621 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7681 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7741 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7801 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7861 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     7921 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7981 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8041 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8101 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8161 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8221 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8281 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8341 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8401 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8461 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagct
//
