LOCUS       Tets                   10216 bp ds-DNA     circular     17-AUG-2020
DEFINITION  .
ACCESSION   pVec1                                                              
VERSION     pVec1.1                                                            
FEATURES             Location/Qualifiers
     rep_origin      7711..8017
                     /label="f1 Origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(6505..6541)
                     /label="H2B-R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    6508..6544
                     /label="Cherry-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6009..6056
                     /label="NLS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    6126..6524
                     /label="H2B"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6525..7229
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7479..7619
                     /label="R-ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1908..6008
                     /label="hSpCsn1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7239..7470
                     /label="bGH polyA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8536..9393
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      9544..10211
                     /label="pUC ori"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1..249
                     /label="Human U6 Promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6057..6062
                     /label="EcoRI"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1788..1856
                     /label="3xFLAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7230..7235
                     /label="EcoRI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6950..6955
                     /label="BBSI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        443..1776
                     /label="EF1a Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    251..270
                     /label="Guide"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    347..352
                     /label="U6 terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1857..1907
                     /label="NLS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6063..6125
                     /label="T2A"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    271..346
                     /label="chimeric guide RNA scaffold"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(250..274)
                     /label="Neurog2 (ENSMUSG00000027967) Assembly 3 REV"
                     /note="sequence: aaacCGGCGACCCTGACCCCGATGC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          246..270
                     /label="Neurog2 (ENSMUSG00000027967) Assembly 3 FWD"
                     /note="sequence: CACCGCATCGGGGTCAGGGTCGCCG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA
      241 CGAAACACCG CATCGGGGTC AGGGTCGCCG gttttagagc taGAAAtagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcTTTT TTgttttaga
      361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
      421 gacaaatggc tctagaggta ccagtaattc atacaaaagg actcgcccct gccttgggga
      481 atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg
      541 ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg
      601 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      661 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      721 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      781 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      841 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      901 cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
      961 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt
     1021 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     1081 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     1141 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     1201 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     1261 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     1321 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     1381 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     1441 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     1501 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     1561 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     1621 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     1681 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     1741 gttcaaagtt tttttcttcc atttcaggtg tcgtgaccgg tgccaccatg gactataagg
     1801 accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac gataagatgg
     1861 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac aagaagtaca
     1921 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     1981 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2041 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2101 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     2161 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     2221 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     2281 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     2341 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     2401 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     2461 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     2521 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     2581 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     2641 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     2701 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     2761 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     2821 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     2881 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     2941 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3001 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3061 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3121 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     3181 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     3241 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     3301 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     3361 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     3421 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     3481 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     3541 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     3601 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     3661 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     3721 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     3781 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     3841 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     3901 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     3961 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4021 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4081 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4141 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     4201 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     4261 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     4321 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     4381 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     4441 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     4501 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     4561 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     4621 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     4681 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     4741 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     4801 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     4861 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     4921 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     4981 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5041 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5101 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     5161 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     5221 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     5281 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     5341 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     5401 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     5461 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     5521 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     5581 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     5641 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     5701 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     5761 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     5821 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     5881 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     5941 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6001 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggaat
     6061 tcggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
     6121 gcccaATGCC TGAACCCTCT AAGTCTGCTC CAGCCCCTAA AAAGGGTTCT AAGAAGGCTA
     6181 TCACTAAGGC GCAGAAGAAG GATGGTAAGA AGCGTAAGCG CAGCCGCAAG GAGAGCTATT
     6241 CTATCTATGT GTACAAGGTT CTGAAGCAGG TCCACCCCGA CACCGGCATC TCATCCAAGG
     6301 CCATGGGGAT CATGAACTCC TTCGTCAACG ACATCTTCGA GCGCATCGCG GGCGAGGCTT
     6361 CTCGCCTGGC TCACTACAAT AAGCGCTCGA CCATCACCTC CAGGGAGATT CAGACGGCTG
     6421 TGCGCCTGCT GCTGCCTGGG GAGCTGGCTA AGCATGCTGT GTCCGAGGGC ACTAAGGCAG
     6481 TTACCAAGTA CACTAGCTCt aaggatccac cggtcgccac catggtgagc aagggcgagg
     6541 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     6601 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     6661 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     6721 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     6781 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     6841 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     6901 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aaaaccatgg
     6961 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     7021 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     7081 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     7141 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     7201 ccaccggcgg catggacgag ctgtacaagg aattctaact agagctcgct gatcagcctc
     7261 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7321 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7381 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7441 ttgggaagag aatagcaggc atgctgggga gcggccgcag gaacccctag tgatggagtt
     7501 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     7561 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     7621 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     7681 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7741 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     7801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     7921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     8041 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     8101 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     8161 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     8221 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     8281 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     8341 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     8401 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8461 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8521 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8581 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8641 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8701 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8761 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8821 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8881 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8941 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9001 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9061 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9121 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9181 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9241 ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9301 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9361 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9421 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9481 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9541 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9601 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9661 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9721 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9781 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9841 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9901 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9961 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10021 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10081 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10141 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10201 ccttttgctc acatgt
//