LOCUS       pX458_Ef1a_Cas9_        9826 bp    DNA        circular     17-AUG-2020
DEFINITION  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        1..249
                     /locus_tag="Human U6 Promoter"
                     /label="Human U6 Promoter"
                     /ApEinfo_label="Human U6 Promoter"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    7089..7229
                     /locus_tag="R-ITR"
                     /label="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1908..6008
                     /locus_tag="hSpCsn1"
                     /label="hSpCsn1"
                     /ApEinfo_label="hSpCsn1"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      7321..7627
                     /locus_tag="f1 Origin"
                     /label="f1 Origin"
                     /ApEinfo_label="f1 Origin"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    8146..9003
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    443..1776
                     /locus_tag="EF1a Promoter"
                     /label="EF1a Promoter"
                     /ApEinfo_label="EF1a Promoter"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6849..7080
                     /locus_tag="bGH polyA"
                     /label="bGH polyA"
                     /ApEinfo_label="bGH polyA"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6063..6125
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6840..6845
                     /locus_tag="EcoRI"
                     /label="EcoRI"
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      9154..9821
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1857..1907
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6057..6062
                     /locus_tag="EcoRI(1)"
                     /label="EcoRI(1)"
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    347..352
                     /locus_tag="U6 terminator"
                     /label="U6 terminator"
                     /ApEinfo_label="U6 terminator"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1788..1856
                     /locus_tag="3xFLAG"
                     /label="3xFLAG"
                     /ApEinfo_label="3xFLAG"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6126..6839
                     /locus_tag="GFP"
                     /label="GFP"
                     /ApEinfo_label="GFP"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    271..346
                     /locus_tag="chimeric guide RNA scaffold"
                     /label="chimeric guide RNA scaffold"
                     /ApEinfo_label="chimeric guide RNA scaffold"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6009..6056
                     /locus_tag="NLS(1)"
                     /label="NLS(1)"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer          711..732
                     /note="sequence: agtgatgtcgtgtactggctcc"
                     /locus_tag="InFusion Construct Ef1a Seq2"
                     /label="InFusion Construct Ef1a Seq2"
                     /ApEinfo_label="InFusion Construct Ef1a Seq2"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer          1724..1745
                     /note="sequence: tcaagcctcagacagtggttca"
                     /locus_tag="InFusion Construct Ef1a Seq4"
                     /label="InFusion Construct Ef1a Seq4"
                     /ApEinfo_label="InFusion Construct Ef1a Seq4"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer          1221..1239
                     /note="sequence: gtcccagcgcacatgttcg"
                     /locus_tag="InFusion Construct Ef1a Seq3"
                     /label="InFusion Construct Ef1a Seq3"
                     /ApEinfo_label="InFusion Construct Ef1a Seq3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccG attacagtag gagcgagggg gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
      361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
      421 gacaaatggc tctagaggta ccagtaattc atacaaaagg actcgcccct gccttgggga
      481 atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg
      541 ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg
      601 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      661 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      721 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      781 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      841 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      901 cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
      961 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt
     1021 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     1081 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     1141 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     1201 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     1261 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     1321 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     1381 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     1441 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     1501 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     1561 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     1621 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     1681 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     1741 gttcaaagtt tttttcttcc atttcaggtg tcgtgaccgg tgccaccatg gactataagg
     1801 accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac gataagatgg
     1861 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac aagaagtaca
     1921 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     1981 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2041 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2101 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     2161 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     2221 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     2281 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     2341 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     2401 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     2461 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     2521 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     2581 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     2641 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     2701 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     2761 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     2821 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     2881 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     2941 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3001 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3061 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3121 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     3181 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     3241 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     3301 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     3361 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     3421 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     3481 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     3541 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     3601 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     3661 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     3721 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     3781 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     3841 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     3901 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     3961 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4021 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4081 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4141 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     4201 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     4261 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     4321 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     4381 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     4441 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     4501 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     4561 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     4621 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     4681 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     4741 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     4801 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     4861 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     4921 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     4981 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5041 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5101 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     5161 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     5221 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     5281 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     5341 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     5401 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     5461 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     5521 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     5581 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     5641 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     5701 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     5761 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     5821 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     5881 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     5941 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6001 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggaat
     6061 tcggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
     6121 gcccagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     6241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6301 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     6361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     6421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     6481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     6541 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6721 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     6841 aattctaact agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6901 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6961 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     7021 tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc atgctgggga
     7081 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     7141 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     7201 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     7261 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     7321 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7381 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7441 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7501 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7561 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7621 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     7681 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7741 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7801 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7861 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7921 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     7981 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8161 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8221 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8281 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8341 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8401 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8461 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8521 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8581 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8641 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8701 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8761 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8821 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca
     8881 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8941 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9001 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9061 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt 
//
