LOCUS       pX458_Ef1a_Cas9_        9825 bp    DNA        circular     17-AUG-2020
DEFINITION  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        1..249
                     /locus_tag="Human U6 Promoter"
                     /label="Human U6 Promoter"
                     /ApEinfo_label="Human U6 Promoter"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    7088..7228
                     /locus_tag="R-ITR"
                     /label="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1907..6007
                     /locus_tag="hSpCsn1"
                     /label="hSpCsn1"
                     /ApEinfo_label="hSpCsn1"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      7320..7626
                     /locus_tag="f1 Origin"
                     /label="f1 Origin"
                     /ApEinfo_label="f1 Origin"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    8145..9002
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    442..1775
                     /locus_tag="EF1a Promoter"
                     /label="EF1a Promoter"
                     /ApEinfo_label="EF1a Promoter"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6848..7079
                     /locus_tag="bGH polyA"
                     /label="bGH polyA"
                     /ApEinfo_label="bGH polyA"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6062..6124
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6839..6844
                     /locus_tag="EcoRI"
                     /label="EcoRI"
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      9153..9820
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1856..1906
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6056..6061
                     /locus_tag="EcoRI(1)"
                     /label="EcoRI(1)"
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    346..351
                     /locus_tag="U6 terminator"
                     /label="U6 terminator"
                     /ApEinfo_label="U6 terminator"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1787..1855
                     /locus_tag="3xFLAG"
                     /label="3xFLAG"
                     /ApEinfo_label="3xFLAG"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6125..6838
                     /locus_tag="GFP"
                     /label="GFP"
                     /ApEinfo_label="GFP"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    270..345
                     /locus_tag="chimeric guide RNA scaffold"
                     /label="chimeric guide RNA scaffold"
                     /ApEinfo_label="chimeric guide RNA scaffold"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6008..6055
                     /locus_tag="NLS(1)"
                     /label="NLS(1)"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer          710..731
                     /note="sequence: agtgatgtcgtgtactggctcc"
                     /locus_tag="InFusion Construct Ef1a Seq2"
                     /label="InFusion Construct Ef1a Seq2"
                     /ApEinfo_label="InFusion Construct Ef1a Seq2"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer          1723..1744
                     /note="sequence: tcaagcctcagacagtggttca"
                     /locus_tag="InFusion Construct Ef1a Seq4"
                     /label="InFusion Construct Ef1a Seq4"
                     /ApEinfo_label="InFusion Construct Ef1a Seq4"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer          1220..1238
                     /note="sequence: gtcccagcgcacatgttcg"
                     /locus_tag="InFusion Construct Ef1a Seq3"
                     /label="InFusion Construct Ef1a Seq3"
                     /ApEinfo_label="InFusion Construct Ef1a Seq3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg tttatgaagc tcggcagagg ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      421 acaaatggct ctagaggtac cagtaattca tacaaaagga ctcgcccctg ccttggggaa
      481 tcccagggac cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct gcgttcccgc
      541 cccctcaccc gcccgctctc gtcatcactg aggtggagaa gagcatgcgt gaggctccgg
      601 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      661 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      721 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      781 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      841 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      901 gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
      961 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg
     1021 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     1081 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     1141 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     1201 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     1261 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     1321 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     1381 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     1441 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     1501 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1561 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1621 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1681 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1741 ttcaaagttt ttttcttcca tttcaggtgt cgtgaccggt gccaccatgg actataagga
     1801 ccacgacgga gactacaagg atcatgatat tgattacaaa gacgatgacg ataagatggc
     1861 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccgaca agaagtacag
     1921 catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
     1981 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
     2041 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
     2101 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
     2161 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
     2221 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     2281 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     2341 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     2401 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     2461 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
     2521 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     2581 cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct
     2641 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     2701 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     2761 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     2821 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     2881 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     2941 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg
     3001 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     3061 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     3121 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     3181 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     3241 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     3301 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     3361 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     3421 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa
     3481 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     3541 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     3601 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     3661 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     3721 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     3781 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     3841 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     3901 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     3961 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     4021 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     4081 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     4141 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     4201 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     4261 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     4321 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     4381 ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt
     4441 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa
     4501 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     4561 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     4621 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     4681 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     4741 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     4801 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     4861 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     4921 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     4981 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     5041 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     5101 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     5161 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     5221 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg
     5281 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
     5341 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     5401 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     5461 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     5521 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
     5581 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     5641 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct
     5701 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     5761 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc
     5821 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     5881 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
     5941 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     6001 aggcgacaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaaggaatt
     6061 cggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
     6121 cccagtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     6181 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     6241 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     6301 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     6361 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     6421 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     6481 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     6541 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     6601 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     6661 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     6721 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     6781 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagga
     6841 attctaacta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6901 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6961 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7021 ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag
     7081 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     7141 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     7201 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     7261 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     7321 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     7381 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7441 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7501 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7561 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7621 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     7681 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7741 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7801 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     7861 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7921 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     7981 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     8041 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8101 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8161 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8221 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8281 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8341 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8401 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8461 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8521 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8581 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8641 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8701 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8761 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8821 gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag
     8881 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8941 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9001 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9061 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9121 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9181 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9241 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9301 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9361 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9421 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9481 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9541 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9601 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9661 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9721 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9781 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt 
//
