LOCUS       pscalps_puro_Hor       10143 bp    DNA     circular UNA 02-SEP-2020
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...2em-c84ek5t
VERSION     urn.local...2em-c84ek5t
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      236..579
     promoter        580..1161
                     /note="CMVieProm"
     5'UTR           1180..1276
                     /note="5' U3"
     5'UTR           1277..1361
                     /note="5' U5"
     misc_feature    1411..1548
                     /note="Geneious type: frag"
                     /note="packaging signal"
     misc_signal     1465..1473
                     /note="5'Splice Donor"
     CDS             1515..1882
                     /note="gag"
     CDS             1883..2738
                     /note="env sequence"
     misc_signal     2026..2265
                     /note="RRE"
     misc_signal     2635..2639
                     /note="3' SA"
     misc_feature    2764..2941
                     /note="Geneious type: frag"
                     /note="Charneau"
     misc_signal     2792..2807
                     /note="cPPT"
     misc_signal     2871..2911
                     /note="Charneau Central Termination Signal"
     misc_signal     2917..2921
                     /note="3'SA"
     promoter        2950..3451
                     /note="SFFVprom"
     misc_feature    3453..5929
                     /note="Geneious type: Concatenated sequence"
     misc_feature    3453..3456
                     /note="Geneious type: ligation"
     misc_feature    <3453..3467
                     /note="Geneious type: Editing History Insertion"
     misc_feature    <3453..3457
     misc_feature    3462..3467
     misc_feature    3474..3476
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3523
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3537..3539
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3548
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3558
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3567
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3569..3570
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3572
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3579
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3588
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3716^3717
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3718..3719
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3884
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3943..3944
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5033
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5223..5224
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5883..>5929
                     /note="Geneious type: Editing History Insertion"
     misc_feature    5892..5921
     misc_feature    5926..3456
                     /note="Geneious type: Concatenated sequence"
     misc_feature    5926..5929
                     /note="Geneious type: ligation"
     promoter        5931..6412
                     /note="CypA"
     misc_feature    6413..7032
     misc_signal     7036..7611
                     /note="WCHV"
     misc_feature    7632..8141
                     /note="Geneious type: frag"
                     /note="nef"
     misc_signal     7803..7818
                     /note="PPT"
     3'UTR           7818..8132
                     /note="3' deltaU3"
     3'UTR           8141..8237
                     /note="3' R"
     3'UTR           8238..8321
                     /note="3' U5"
     oriT            8332..9002
                     /note="colE1"
     CDS             complement(9093..9993)
                     /note="Ampicillin resistance"
ORIGIN      
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgctgtg
      241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      481 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      541 aggaggcttt tttggaggcc taggcttttg caaaaagctt tgacattgat tattgactag
      601 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      661 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      721 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      781 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      841 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      901 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      961 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1021 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1081 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1141 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     1201 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1261 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1321 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1381 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1441 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1501 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1561 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1621 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1681 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1741 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1801 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1861 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1921 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1981 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2041 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2101 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2161 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2221 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2281 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2341 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     2401 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     2461 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2521 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2581 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2641 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2701 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     2761 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2821 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2881 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     2941 gttaattaac tgcagccccg ataaaataaa agattttatt tagtctccag aaaaaggggg
     3001 gaatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca ttttgcaagg
     3061 catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt acatgaaaat
     3121 agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg gcccggggcc
     3181 aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag atggtcccca
     3241 gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag gctcccccaa
     3301 ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt ctcgcttctg
     3361 ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca ctcggcgcgc
     3421 cagtcctccg acagactgag tcgcccgggg gtctagaagc cgccaccatg agctctagct
     3481 catggctgct cctgagcttg gttgccgtca cgacggcaca gtttacgaca gaagatctcg
     3541 caaaaatttt tttggatgag ttcaattccg aagcagagaa tttgtcatac caatccagcc
     3601 ttgccagctg ggactataac acaaatatca atgatgaaaa cgttcaaaaa atggatgaag
     3661 cgggcgcaaa atggtccgcg ttttatgaag aacaatccaa actcgctaaa aactatccac
     3721 tggaacaaat acaaaatgtg actgtcaaac tgcagctgca aatcttgcaa caaagtggct
     3781 caccagtact ttctgaggac aaaagcaagc gactcaattc aattctgaat gcgatgtcca
     3841 ccatctactc aacgggaaaa gtttgcaaac cgaacaagcc acatgagtgt ttgctccttg
     3901 agcccgggct ggataatata atgggtacca gcaaagacta tagcgaaagg ctgtgggcct
     3961 gggaagggtg gagggcagag gtaggaaagc agctgcggcc actgtacgaa gagtatgtcg
     4021 ttctcaaaaa cgaaatggct cgcgggtatc actatgaaga ctacggggac tactggaggc
     4081 gggattatga aaccgaggag tccccagggc cgggttactc aagggaccag ctcatgaagg
     4141 acgtcgagcg catcttcaca gagatcaagc cgctctatga gcatcttcac gcctacgttc
     4201 gagcgaagct tatggacaca tatccgttcc atatatctcc aacgggctgc ctgccagcac
     4261 atcttctggg cgatatgtgg ggtcgattct ggacgaacct ttaccctctc acagtaccgt
     4321 tcgggcaaaa gccgaatata gacgttacgg atgagatgct caagcagggt tgggatgctg
     4381 atcggatatt caaggaagca gaaaagtttt ttgtatctgt tgggcttccc aatatgaccg
     4441 aaggattctg gaataattcc atgcttaccg agcccggaga cggtcgcaaa gtggtttgcc
     4501 accctacggc ctgggatctt ggtaaaggag attttcgaat aaaaatgtgc actaaagtaa
     4561 ccatggaaga cttcttgaca gcgcatcatg agatgggaca catacagtac gacatggcct
     4621 atgctagtca gccctatctc ttgcggaacg gggccaacga aggtttccat gaagcggtcg
     4681 gcgaagtgat gtccctttca gtagcgactc ccaaacactt gaagactatg ggacttctca
     4741 gcccggactt cagggaagat aatgagactg aaataaactt tcttcttaag caggccctca
     4801 atattgttgg tacacttccg tttacttaca tgctggaaaa atggaggtgg atggtcttca
     4861 agggcgaaat tccgaaggag gaatggatga agaaatggtg ggagatgaag cggaagattg
     4921 taggggtagt agagcctgtt ccgcatgatg aaacatattg tgacccggcg tccctttttc
     4981 atgtcgccaa tgattacagc ttcatccgct attatacgag aactattttc gagttccaat
     5041 ttcacgaagc cttgtgcagg attgctcaac atgacggacc ccttcataaa tgcgatatat
     5101 ccaacagtac ggatgcaggg aaaaagttgc atcaaatgct ctccgtggga aaaagccaag
     5161 cctggacgaa gacgcttgaa gatatcgtag attcacgaaa catggacgtt ggtccacttc
     5221 tccgatactt tgaaccactt tacacgtggc tgcaagagca gaatcgcaag tcctatgttg
     5281 gctggaacac agattggagt ccgtacagtg atcagtccat caaggtgcgg atctcactca
     5341 agtctgcgtt gggggagaac gcatatgagt ggaacgacaa cgaaatgtat cttttccgct
     5401 caagtgttgc gtacgcgatg agggaatatt tcctcaaaga aaaacatcaa actattctct
     5461 ttggcgctga aaatgtctgg gtctccaatt tgaaaccacg aattagcttt aactttcatg
     5521 taaccagtcc cggaaatctc tccgacatca taccgaggcc cgaggtcgaa ggtgcgatac
     5581 ggatgagcag gagtaggatt aatgacgcat ttcgcctgga tgacaactcc ctcgagttcc
     5641 ttggcataca accgacgctc gggccaccct accaacctcc ggtcacgatc tggctgatcg
     5701 tgtttggcgt agttatggca gttgttgtag tgggcattgt tgtactcatc atcacaggca
     5761 tacgagacag aagaaaaaca gaccaggcaa ggtcagagga gaacccttat agttccgttg
     5821 accttagtaa aggtgagaat aatcccggtt ttcagaacgg cgacgacgtc caaacgtcat
     5881 ttggagcagc tgagcagaag ctgatcagcg aggaggacct gtaggaattc tcgaccttga
     5941 ctggcggcgc gaccttgagg cctgcgttcg cctcagttgc cccctctgtg caatggggag
     6001 acgcgcctca tcgcttgaca acggccgaag agccgccgcg cttccgtctc ccgcgtgcgc
     6061 gcgccatgct gcccaccccc gttccgcact gaccctcccc cgtgccccgc gtcccgtact
     6121 gccgccccgc cccgagtccc atgccgcagc caccgcgacg gagcccgcag gcgggaacct
     6181 gcctccgcgc gttagcgcgc acgcgcgcct catgtgtcgt ccccatcagc gccggcttcc
     6241 gtctataggc cagatgcact gtcactctgg cgaagtcgca gacccgattg gccgggacgg
     6301 aggcgcgaga ccgggttgcg ggcggggccg aacgtggtat aaaaggggcg ggaggccagg
     6361 ctcgtgccgt tttgcagacg ccaccgccga ggaaaaccgt gtactattag ccacgcgtgc
     6421 cgccatgacc gaatacaaac ctaccgtgag gctggctaca agagatgatg tcccaagggc
     6481 tgtgagaaca ctggccgccg cttttgccga ttaccctgcc acacgccaca ctgtggaccc
     6541 agatcggcat atcgagagag tgactgagct gcaggaactg ttcctgaccc gagtgggcct
     6601 ggacattggg aaggtctggg tcgcagacga tggagcagct gtggctgtct ggaccacacc
     6661 agagagcgtg gaagccggag ctgtctttgc agagatcggc cctagaatgg cagaactgag
     6721 cggctccagg ctggcagcac agcagcagat ggagggactg ctggccccac acaggcctaa
     6781 ggaaccagca tggttcctgg ctaccgtggg ggtctctcct gaccatcagg gcaaaggact
     6841 gggaagtgct gtggtcctgc caggagtgga ggctgcagaa cgagctggag tccctgcatt
     6901 tctggagacc tctgctccac gaaacctgcc cttctatgaa cggctgggct ttactgtgac
     6961 cgcagatgtg gaggtccccg aaggacctag gacctggtgc atgacacgca aacccggcgc
     7021 ctgagcgatc gctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7081 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7141 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7201 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7261 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7321 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7381 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7441 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7501 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7561 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7621 gcttaatcgc gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7681 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7741 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7801 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7861 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     7921 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     7981 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     8041 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     8101 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     8161 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8221 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8281 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt catgtgagca
     8341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8581 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8821 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8941 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9001 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9061 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10141 gac
//
