LOCUS       Exported               13250 bp ds-DNA     circular SYN 19-AUG-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 19, 2020 from SnapGene 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     mobile_element  complement(1..701)
                     /mobile_element_type="transposon"
                     /label=piggyBacL
                     /note="color: #8aa6f7; direction: LEFT"
     primer_bind     complement(866..882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(866..882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(879..901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    890..906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(914..944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 914 .. 920 / #ffffff / -10
                        2: 921 .. 938 / #ffffff
                        3: 939 .. 944 / #ffffff / -35"
     protein_bind    959..980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(1097..1114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(1268..1856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(1348..1367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(2027..2887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2027 .. 2818 / #ccffcc
                        2: 2819 .. 2887 / #ccffcc / signal sequence
                     Cleavage site after base 2818"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2650..2669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(2888..2992)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     3060..3078
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
     primer_bind     complement(3116..3138)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
                     /note="color: black; sequence: CCGGGAGCTGCATGTGTCAGAGG"
     primer_bind     3238..3257
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     primer_bind     3451..3473
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     3465..3482
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     3466..3482
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     repeat_region   4186..4214
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
                     /note="color: #ffcc99"
     mobile_element  complement(4215..5212)
                     /mobile_element_type="transposon"
                     /label=piggyBacR
                     /note="color: #8aa6f7; direction: LEFT"
     misc_binding    5261..5545
                     /label=attB site
                     /note="attB site"
                     /note="color: #8aa6f7"
     primer_bind     complement(5261..5292)
                     /label=NK=252L-2R
                     /note="color: black; sequence: 
                     gcggcttcgagaccgtgacctacatcgtcgac"
     primer_bind     5261..5284
                     /label=GTCGACGATGTAGGTCACGGTCTC
                     /note="color: black; sequence: GTCGACGATGTAGGTCACGGTCTC"
     primer_bind     5521..5580
                     /label=bTub.1F
                     /note="color: black; sequence: 
                     cgacggtcacggcgggcatgtcgacgcggccgcTCGACCCATCCCATTATACACCCATA
                     T; added: 2020-03-23"
     misc_feature    5554..6034
                     /label=dme_beta_tubulin Promoter
                     /note="dme_beta_tubulin Promoter"
                     /note="color: #00ff00"
     primer_bind     complement(6008..6067)
                     /label=bTub.2R
                     /note="color: black; sequence: 
                     GTCTCCGTCGTGGTCCTTATAGTCCATctcgagTTTGATAGTAAAGTTAGGGCCCCTTT
                     T; added: 2020-03-23"
     CDS             6044..6109
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 6109"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6116..6136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    6161..10261
                     /label=hSpCas9
                     /note="color: #ff8000"
     CDS             10262..10309
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(10288..10309)
                     /label=TTACTTTTTCTTTTTTGCCTGGCCG
                     /note="color: black; sequence: TTACTTTTTCTTTTTTGCCTGGCCG"
     CDS             10310..10363
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 10360"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10364..11083
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 10364 .. 10366 / #05fd14
                        2: 10367 .. 10369 / #05fd14 / 1a
                        3: 10370 .. 11083 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     10364..10384
                     /label=ATGGTGAGCAAGGGCGAGGAG
                     /note="color: black; sequence: ATGGTGAGCAAGGGCGAGGAG"
     primer_bind     complement(11060..11083)
                     /label=TTACTTGTACAGCTCGTCCATGCC
                     /note="color: black; sequence: TTACTTGTACAGCTCGTCCATGCC"
     primer_bind     11092..11123
                     /label=CTAGAATGAATCGTTTTTAAAATAACAAATCA
                     /note="color: black; sequence: 
                     CTAGAATGAATCGTTTTTAAAATAACAAATCA"
     3'UTR           11097..11756
                     /label=p10 3'UTR
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(11749..11768)
                     /label=CCGGCCGTTAACTCGAATCG
                     /note="color: black; sequence: CCGGCCGTTAACTCGAATCG"
     promoter        11777..12317
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
                     /note="color: #99ccff; direction: RIGHT"
     primer_bind     11777..11797
                     /label=CATACTCGGTGGCCTCCCCAC
                     /note="color: black; sequence: CATACTCGGTGGCCTCCCCAC"
     primer_bind     complement(12212..12238)
                     /label=AACAGATGCTGTTCAACTGTGTTTACC
                     /note="color: black; sequence: AACAGATGCTGTTCAACTGTGTTTACC"
     primer_bind     complement(12264..12282)
                     /label=TGGGCGCGTTGGCGTTGTT
                     /note="color: black; sequence: TGGGCGCGTTGGCGTTGTT"
     primer_bind     complement(12298..12317)
                     /label=CACCAGAGACAGGTTGCGGC
                     /note="color: black; sequence: CACCAGAGACAGGTTGCGGC"
     misc_feature    12318..12998
                     /label=Dsred
                     /note="Dsred"
                     /note="color: #ff0000; direction: RIGHT"
     3'UTR           12999..13239
                     /label=Sv40
                     /note="Sv40"
                     /note="color: #99ccff; direction: RIGHT"
     primer_bind     12999..13025
                     /label=GACTCTAGATCATAATCAGCCATACCA
                     /note="color: black; sequence: GACTCTAGATCATAATCAGCCATACCA"
     primer_bind     complement(13210..13239)
                     /label=AAGCTTTAAGATACATTGATGAGTTTGGAC
                     /note="color: black; sequence: 
                     AAGCTTTAAGATACATTGATGAGTTTGGAC"
ORIGIN
        1 gatctcggat ctgacaatgt tcagtgcaga gactcggcta cgcctcgtgg actttgaagt
       61 tgaccaacaa tgtttattct tacctctaat agtcctctgt ggcaaggtca agattctgtt
      121 agaagccaat gaagaacctg gttgttcaat aacattttgt tcgtctaata tttcactacc
      181 gcttgacgtt ggctgcactt catgtacctc atctataaac gcttcttctg tatcgctctg
      241 gacgtcatct tcacttacgt gatctgatat ttcactgtca gaatcctcac caacaagctc
      301 gtcatcgctt tgcagaagag cagagaggat atgctcatcg tctaaagaac tacccatttt
      361 attatatatt agtcacgata tctataacaa gaaaatatat atataataag ttatcacgta
      421 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
      481 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
      541 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
      601 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
      661 ttttacgcag actatctttc tagggttaaa aaagatttgc gctttactcg acctaaactt
      721 taaacacgtc atagaatctt cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc
      781 gacgcgcgct aaagaatggc aaaccaagtc gcgcgagcgt cgactctaga ggatccccgg
      841 gtaccgagct cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
      901 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      961 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1021 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1081 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1141 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1201 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1261 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1321 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1381 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1441 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1501 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1561 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1621 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1681 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1741 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1801 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1861 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1921 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1981 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2041 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2101 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2161 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2221 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2281 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2341 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2401 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2461 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2521 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2581 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2641 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2701 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2761 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2821 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2881 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2941 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3001 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     3061 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     3121 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     3181 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     3241 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     3301 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     3361 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3421 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3481 gtgccaagct ttgtttaaaa atataacaaa attgtgatcc cacaaaatga agtggggcaa
     3541 aatcaaataa ttaatagtgt ccgtaaactt gttggtcttc aactttttga ggaacacgtt
     3601 ggacggcaaa tccgtgacta taacacaagt tgatttaata attttagcca acacgtcggg
     3661 ctgcgtgttt tttgccgacg cgtctgtgta cacgttgatt aactggtcga ttaaactgtt
     3721 gaaataattt aatttttggt tcttctttaa atctgtgatg aaatttttta aaataacttt
     3781 aaattcttca ttggtaaaaa atgccacgtt ttgcaacttg tgagggtcta atatgaggtc
     3841 aaactcagta ggagttttat ccaaaaaaga aaacatgatt acgtctgtac acgaacgcgt
     3901 attaacgcag agtgcaaagt ataagagggt taaaaaatat attttacgca ccatatacgc
     3961 atcgggttga tatcgttaat atggatcaat ttgaacagtt gattaacgtg tctctgctca
     4021 agtctttgat caaaacgcaa atcgacgaaa atgtgtcgga caatatcaag tcgatgagcg
     4081 aaaaactaaa aaggctagaa tacgacaatc tcacagacag cgttgagata tacggtattc
     4141 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
     4201 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
     4261 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
     4321 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
     4381 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
     4441 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     4501 gcttaactta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     4561 cacatatcaa tattatgctc tcgacaaata acttttttgc attttttgca cgatgcattt
     4621 gcctttcgcc ttattttaga ggggcagtaa gtacagtaag tacgtttttt cattactggc
     4681 tcttcagtac tgtcatctga tgtaccaggc acttcatttg gcaaaatatt agagatatta
     4741 tcgcgcaaat atctcttcaa agtaggagct tctaaacgct tacgcataaa cgatgacgtc
     4801 aggctcatgt aaaggtttct cataaatttt ttgcgacttt gaaccttttc tcccttgcta
     4861 ctgacattat ggctgtatat aataaaagaa tttatgcagg caatgtttat cattccgtac
     4921 aataatgcca taggccacct attcgtcttc ctactgcagg tcatcacaga acacatttgg
     4981 tctagcgtgt ccactccgcc tttagtttga ttataataca taaccatttg cggtttaccg
     5041 gtactttcgt tgatagaagc atcctcatca caagatgata ataagtatac catcttagct
     5101 ggcttcggtt tatatgagac gagagtaagg ggtccgtcaa aacaaaacat cgatgttccc
     5161 actggcctgg agcgactgtt tttcagtact tccggtatct cgcgtttgtt tgatcgcacg
     5221 gttcccacaa tggttaattc gagctcgccc ggggtcctag gtcgacgatg taggtcacgg
     5281 tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca
     5341 cctcacccat ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga
     5401 acgcgcggcg caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga
     5461 gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc agcgggttct
     5521 cgacggtcac ggcgggcatg tcgacgcggc cgctcgaccc atcccattat acacccatat
     5581 aaacatttac aaaggagtaa aatccaggac atccatgtca atatcaatcg tatcatctgg
     5641 tcggtagcct tggaatcctc tattgcttcc aaggcaccgc caaatccatc ccatctcgaa
     5701 ttttagccgt atattcgttt atctatgtaa gtactattaa agtttgtgct caaaacggag
     5761 aactgagttt tctgaaatcg gggtgtgtga aatgtgtcga agtcggaaat cgtagtagcc
     5821 tatttgtgaa cattcggtgt agtaatccaa gccaggttca gttcacctca gtatcagcta
     5881 gcacgtacac gactaaaatc taaacctgaa aaattatacg tttaaatatt cagtcttttg
     5941 ccgatttttg ccccactcag actgttttaa aagctcgatt tttttttgta ccattttttc
     6001 ggtgtgaaaa aggggcccta actttactat caaactcgag atggactata aggaccacga
     6061 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
     6121 gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg
     6181 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     6241 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     6301 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     6361 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     6421 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     6481 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     6541 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     6601 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     6661 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     6721 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     6781 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     6841 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     6901 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     6961 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     7021 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     7081 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     7141 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     7201 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     7261 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     7321 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     7381 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     7441 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     7501 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     7561 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     7621 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     7681 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     7741 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     7801 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     7861 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     7921 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     7981 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     8041 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     8101 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     8161 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     8221 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     8281 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     8341 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     8401 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     8461 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     8521 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     8581 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     8641 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     8701 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     8761 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     8821 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     8881 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     8941 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     9001 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     9061 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     9121 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     9181 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     9241 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     9301 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     9361 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     9421 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     9481 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     9541 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     9601 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     9661 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     9721 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     9781 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     9841 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     9901 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     9961 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
    10021 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
    10081 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
    10141 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
    10201 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
    10261 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg agggcagagg
    10321 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatggtga gcaagggcga
    10381 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
    10441 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
    10501 gttcatctgc accaccagca agctgcccgt gccctggccc accctcgtga ccaccctgac
    10561 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
    10621 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
    10681 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
    10741 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
    10801 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
    10861 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
    10921 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
    10981 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
    11041 cgccgccggg atcactctcg gcatggacga gctgtacaag taattaatta actagaatga
    11101 atcgttttta aaataacaaa tcaattgttt tataatattc gtacgattct ttgattatgt
    11161 aataaaatgt gatcattagg aagattacga aaaatataaa aaatatgagt tctgtgtgta
    11221 taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg attatttgat
    11281 taaaattgtt gttttctttg ttcatagaca atagtgtgtt ttgcctaaac gtgtactgca
    11341 taaactccat gcgagtgtat agcgagctag tggctaacgc ttgccccacc aaagtagatt
    11401 cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa catttggccg tcggaattaa
    11461 cttctaaaga tgccacataa tctaataaat gaaatagaga ttcaaacgtg gcgtcatcgt
    11521 ccgtttcgac catttccgaa aagaactcgg gcataaactc tatgatttct ctggacgtgg
    11581 tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg cgacatgtcg tcgggaaact
    11641 cgcgcggaaa catgttgttg taaccgaacg ggtcccatag cgccaaaacc aaatctgcca
    11701 gcgtcaatag aatgagcacg atgccgacaa tggagctggc ttggatagcg attcgagtta
    11761 acggccgggg cgcgcccata ctcggtggcc tccccaccac caactttttt gcactgcaaa
    11821 aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc gtagactatt
    11881 ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac attgaacctt
    11941 tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc gggtcgcgtc
    12001 ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt gacaggacgc
    12061 cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca ggacgcgcct
    12121 ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc gtcccgctta
    12181 tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag catctgttac
    12241 agcgacacaa catgagccgg tccaacaacg ccaacgcgcc cacgccatcc aaccgccgcc
    12301 gcaacctgtc tctggtgatg gtgcgctcct ccaagaacgt catcaaggag ttcatgcgct
    12361 tcaaggtgcg catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg
    12421 agggccgccc ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc
    12481 tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga
    12541 agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg
    12601 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
    12661 aggacggctg cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc
    12721 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
    12781 acggcgtgct gaagggcgag atccacaagg ccctgaagct gaaggacggc ggccactacc
    12841 tggtggagtt caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact
    12901 acgtggactc caagctggac atcacctccc acaacgagga ctacaccatc gtggagcagt
    12961 acgagcgcac cgagggccgc caccacctgt tcctgtagga ctctagatca taatcagcca
    13021 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    13081 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    13141 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    13201 ttgtggtttg tccaaactca tcaatgtatc ttaaagcttc taggccggcc
//
