LOCUS       Exported                5240 bp ds-DNA     circular SYN 14-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Vanessa Wall
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 19, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        24..115
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     protein_bind    160..184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             187..696
                     /codon_start=1
                     /gene="Kz-Hs.KRAS(1-169)"
                     /label=Hs.KRAS4b(1-169)
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEK"
     protein_bind    complement(695..719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    851..985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  1014..1179
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1362..1817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1844..1948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1949..2809
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2980..3568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  3871..4095
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4162..4695)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4884..4912)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa
       61 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc
      121 ggattattca taccgtccca ccatcgggcg cggatcatca caagtttgta caaaaaagtt
      181 ggcaccatga ctgaatataa acttgtggta gttggagctg gtggcgtagg caagagtgcc
      241 ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat
      301 tcctacagga agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca
      361 gcaggtcaag aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt
      421 ctttgtgtat ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa
      481 caaattaaaa gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt
      541 gatttgcctt ctagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga
      601 attcctttta ttgaaacatc agcaaagaca agacagggtg ttgatgatgc cttctataca
      661 ttagttcgag aaattcgaaa acataaagaa aagtaatcaa ctttcttgta caaagtggtg
      721 atagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt
      781 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      841 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      961 caatgtatct tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga
     1021 taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg
     1081 ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt
     1141 tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt
     1201 tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt
     1261 tttctctgtc acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg
     1321 actgaatatc aacgcttatt tgcagcctga atggcgaatg gacgcgccct gtagcggcgc
     1381 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     1441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1501 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1561 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     1681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     1741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     1801 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1921 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1981 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2041 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2101 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2161 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2221 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2281 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2341 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2401 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2461 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2521 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2581 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2641 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2701 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2761 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2881 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3001 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3061 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3121 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3181 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3361 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3421 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3481 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3541 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3601 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3661 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3721 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     3781 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     3841 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     3901 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     3961 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     4021 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     4081 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     4141 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     4201 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     4261 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     4321 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     4381 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     4441 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     4501 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     4561 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     4621 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     4681 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     4741 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     4801 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     4861 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     4921 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     4981 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     5041 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     5101 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     5161 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     5221 atagttctag tggttggcta
//
