LOCUS N-ybbR_Tau_pOmniBac 6420 bp ds-DNA circular 28-JAN-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature complement(4262..4293) /label="LoxP" /ApEinfo_revcolor=#ff081d /ApEinfo_fwdcolor=#ff081d misc_feature complement(1275..2107) /label="Ori" /ApEinfo_revcolor=#ff081d /ApEinfo_fwdcolor=#ff081d misc_feature 3152..3350 /label="Tn7R" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 1456..1602 /label="pMB1 ori (from pUC19, no ROP)" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS complement(3440..4192) /label="PromGenCDS" /ApEinfo_revcolor=#827cb4 /ApEinfo_fwdcolor=#827cb4 enhancer 555..1266 /label="1629" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature complement(1495..1602) /label="RNAI" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 4975..5064 /label="Linker TEV" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature complement(330..352) /label="T7R (T7 terminator primer)" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 6..13 /label="mutiple cloning site" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 enhancer 2163..3127 /label="lef2-603" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd polyA_signal complement(73..264) /label="SV40 late polyA" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature join(5101..6420,1..3) /label="Tau" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 5821..6198 /label="MTBD" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 5767..5787 /label="Feinstein" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 C_region 388..554 /label="Tn7L" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 5917..5940 /label="R1-R2 IR" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 4561..4974 /label="ZZ" /ApEinfo_revcolor=#ff0080 /ApEinfo_fwdcolor=#ff0080 misc_feature 5065..5100 /label="ybbR" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1604..2044 /label="pMB1 ori (from pUC19, no ROP)" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 4300..4325 /label="I-CeuI" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_recomb 4263..4296 /label="LoxP" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e ORIGIN 1 taatgagctt gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt 61 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 121 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 181 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 241 catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg 301 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat 361 aacccctagg gtatacccat ctaattggaa ccagataagt gaaatctagt tccaaactat 421 tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg 481 tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat 541 tttgtccgcc cacaaccggt ggaggaaatt ctccttgaag tttccctggt gttcaaagta 601 aaggagtttg caccagacgc acctctgttc actggtccgg cgtattaaaa cacgatacat 661 tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt 721 tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa 781 atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg 841 taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta 901 tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct 961 ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta 1021 tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg 1081 ttaaatagag cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg 1141 tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca 1201 cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt 1261 ggaattaccg gttgacttgg gtcaactgtc agaccaagtt tactcatata tactttagat 1321 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 1381 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1441 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1501 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1561 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 1621 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1681 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1741 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1801 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 1861 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 1921 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 1981 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 2041 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2101 catgttcttt cctgcgttat cccctgattg acttgggtcg ctcttcctgt ggatgcgcag 2161 gtatgtacag gaagaggttt atactaaact gttacattgc aaacgtggtt tcgtgtgcca 2221 agtgtgaaaa ccgatgttta atcaaggctc tgacgcattt ctacaaccac gactctaagt 2281 gtgtgggtga agtcatgcat cttttaatca aatcccaaga tgtgtataaa ccaccaaact 2341 gccaaaaaat gaaaactgtc gacaagctct gtccgtttgc tggcaactgc aagggtctca 2401 atcctatttg taattattga ataataaaac aattataaat gtcaaatttg ttttttatta 2461 acgatacaaa ccaaacgcaa caagaacatt tgtagtatta tctataattg aaaacgcgta 2521 gttataatcg ctgaggtaat atttaaaatc attttcaaat gattcacagt taatttgcga 2581 caatataatt ttattttcac ataaactaga cgccttgtcg tcttcttctt cgtattcctt 2641 ctctttttca tttttctctt cataaaaatt aacatagtta ttatcgtatc catatatgta 2701 tctatcgtat agagtaaatt ttttgttgtc ataaatatat atgtcttttt taatggggtg 2761 tatagtaccg ctgcgcatag tttttctgta atttacaaca gtgctatttt ctggtagttc 2821 ttcggagtgt gttgctttaa ttattaaatt tatataatca atgaatttgg gatcgtcggt 2881 tttgtacaat atgttgccgg catagtacgc agcttcttct agttcaatta caccattttt 2941 tagcagcacc ggattaacat aactttccaa aatgttgtac gaaccgttaa acaaaaacag 3001 ttcacctccc ttttctatac tattgtctgc gagcagttgt ttgttgttaa aaataacagc 3061 cattgtaatg agacgcacaa actaatatca caaactggaa atgtctatca atatatagtt 3121 gctgattgcg cagatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga 3181 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg 3241 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac 3301 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat 3361 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 3421 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 3481 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 3541 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag 3601 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 3661 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc 3721 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 3781 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 3841 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 3901 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 3961 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 4021 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga 4081 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 4141 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc 4201 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 4261 ccataacttc gtatagcata cattatacga agttatctgt aactataacg gtcctaaggt 4321 agcgagttta aacactagta tcgattcgcg acctactccg gaatattaat agatcatgga 4381 gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt 4441 tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc 4501 ggatctcggt ccgaaaccAT GTCGTACTAC CATCACCATC ACCATCACca tcacggttct 4561 ATGTGCatgg caggccttgc gcaacacgat gaagccgtgg acaacaaatt caacaaagaa 4621 caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac 4681 gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct 4741 aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa 4801 aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg aaacgccttc 4861 atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga agctaaaaag 4921 ctaaatgatg ctcaggcgcc gaaagtagac gcaaattccg cggggaagtc aaccgattat 4981 gatattccaa ctactgctag cgagaaccta tatttccagg gaGATTATGA TATCCCTACG 5041 ACTGAGAATC TTTATTTTCA GGGCatgGAC TCATTGGAGT TTATCGCATC AAAGCTCGCA 5101 gctgagcccc gccaggagtt cgaagtgatg gaagatcacg ctgggacgta cgggttgggg 5161 gacaggaaag atcagggggg ctacaccatg caccaagacc aagagggtga cacggacgct 5221 ggcctgaaag aatctcccct gcagaccccc actgaggacg gatctgagga accgggctct 5281 gaaacctctg atgctaagag cactccaaca gcggaagatg tgacagcacc cttagtggat 5341 gagggagctc ccggcaagca ggctgccgcg cagccccaca cggagatccc agaaggaacc 5401 acagctgaag aagcaggcat tggagacacc cccagcctgg aagacgaagc tgctggtcac 5461 gtgacccaag ctcgcatggt cagtaaaagc aaagacggga ctggaagcga tgacaaaaaa 5521 gccaaggggg ctgatggtaa aacgaagatc gccacaccgc ggggagcagc ccctccaggc 5581 cagaagggcc aggccaacgc caccaggatt ccagcaaaaa ccccgcccgc tccaaagaca 5641 ccacccagct ctggtgaacc tccaaaatca ggggatcgca gcggctacag cagccccggc 5701 tccccaggca ctcccggcag ccgctcccgc accccgtccc ttccaacccc acccacccgg 5761 gagcccaaga aggtggcagt ggtccgtact ccacccaagt cgccgtcttc cgccaagagc 5821 cgcctgcaga cagcccccgt gcccatgcca gacctgaaga atgtcaagtc caagatcggc 5881 tccactgaga acctgaagca ccagccggga ggcgggaagg tgcagataat taataagaag 5941 ctggatctta gcaacgtcca gtccaagtgt ggctcaaagg ataatatcaa acacgtcccg 6001 ggaggcggca gtgtgcaaat agtctacaaa ccagttgacc tgagcaaggt gacctccaag 6061 tgtggctcat taggcaacat ccatcataaa ccaggaggtg gccaggtgga agtaaaatct 6121 gagaagcttg acttcaagga cagagtccag tcgaagattg ggtccctgga caatatcacc 6181 cacgtccctg gcggaggaaa taaaaagatt gaaacccaca agctgacctt ccgcgagaac 6241 gccaaagcca agacagacca cggggcggag atcgtgtaca agtcgccagt ggtgtctggg 6301 gacacgtctc cacggcatct cagcaatgtc tcctccaccg gcagcatcga catggtagac 6361 tcgccccagc tcgccacgct agctgacgag gtgtctgcct ccctggccaa gcagggtttg //