LOCUS       ybbR_MAP7_pOmniBac      7344 bp ds-DNA     circular     16-JUL-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     C_region        3433..3599
                     /label="Tn7L"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(4320..5152)
                     /label="Ori"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     CDS             complement(6485..7237)
                     /label="PromGenCDS"
                     /ApEinfo_revcolor=#827cb4
                     /ApEinfo_fwdcolor=#827cb4
     misc_feature    complement(7307..7338)
                     /label="LoxP"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     misc_feature    3051..3058
                     /label="mutiple cloning site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4649..5089
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    802..3048
                     /label="MAP7"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     enhancer        5208..6172
                     /label="lef2-603"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    976..1308
                     /label="MTBD"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    766..801
                     /label="ybbR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    676..765
                     /label="Linker TEV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1..26
                     /label="I-CeuI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_recomb     7308..7341
                     /label="LoxP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    complement(3014..3073)
                     /label="primer"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6197..6395
                     /label="Tn7R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     polyA_signal    complement(3118..3309)
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1327..1746
                     /label="P123"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    262..675
                     /label="ZZ"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     enhancer        3600..4311
                     /label="1629"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4540..4647)
                     /label="RNAI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4501..4647
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3375..3397)
                     /label="T7R (T7 terminator primer)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 taactataac ggtcctaagg tagcgagttt aaacactagt atcgattcgc gacctactcc
       61 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
      121 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
      181 taccgtccca ccatcgggcg cggatctcgg tccgaaaccA TGTCGTACTA CCATCACCAT
      241 CACCATCACc atcacggttc tATGTGCatg gcaggccttg cgcaacacga tgaagccgtg
      301 gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac
      361 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
      421 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac
      481 aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac
      541 gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac
      601 cttttagcag aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc
      661 gcggggaagt caaccgatta tgatattcca actactgcta gcgagaacct atatttccag
      721 ggaGATTATG ATATCCCTAC GACTGAGAAT CTTTATTTTC AGGGCatgGA CTCATTGGAG
      781 TTTATCGCAT CAAAGCTCGC Agcggagcta ggagctggcg gcgacggcca caggggcggc
      841 gacggcgcag tgcgaagcga aacagcaccc gacagctaca aagtgcaaga taagaaaaat
      901 gcctccagcc gccctgcctc tgcaatttca ggacaaaata acaaccactc aggaaataaa
      961 ccagaccctc cgcctgtgtt acgtgttgat gaccggcagc ggctggcccg ggagcgacgt
     1021 gaggaacggg agaaacagct agctgcaaga gaaatagtgt ggttagaaag agaagagcga
     1081 gccaggcagc actacgagaa gcacctggaa gagcggaaga agaggttgga ggagcagagg
     1141 cagaaggagg agcggaggag ggctgctgtg gaggagaagc ggaggcagag acttgaggag
     1201 gacaaagaac gccacgaagc tgttgtacgg cgcacaatgg aaaggagcca gaagccaaaa
     1261 cagaagcata accgttggtc gtggggaggc tctctccatg ggagccctag catccacagt
     1321 gcagatccag acaggcggtc agtttccacc atgaatcttt cgaaatatgt tgatcccgtc
     1381 attagcaagc ggctctcctc ttcatctgca actttactaa attctccaga tagagctcgc
     1441 cgcctgcagc tcagcccatg ggagagcagc gttgttaaca gactcctgac gcccacacat
     1501 tcgttcctgg ccagaagtaa aagcacagct gccttgtctg gagaagcagc atcttgcagc
     1561 cccatcatca tgccctacaa agctgcacac tctagaaatt cgatggatcg accaaaactc
     1621 tttgtaacac cacctgaggg ctcttctcgc aggaggatca ttcatggcac agcgagctat
     1681 aaaaaagaaa gagagagaga aaatgtactc ttcctcacat ctggcacccg aagggctgta
     1741 tctccatcta atcccaaagc aagacaacca gctcgctccc gactttggct tccgtccaag
     1801 tctcttcctc atttgcctgg cacacccaga ccgacatcct ccttgccacc cggctcagtc
     1861 aaagctgctc ctgctcaggt ccggccccca tcccccggca acatccgccc tgtcaagagg
     1921 gaagtcaaag tggagcctga gaagaaagat cctgagaagg aacctcagaa agttgccaat
     1981 gagccctcac taaagggcag agcaccttta gtgaaggtag aagaagccac agttgaagag
     2041 cggacacctg ctgaaccaga agttggccct gctgctccag ccatggcccc agctccagcc
     2101 tcggccccag ctccagcctc ggccccagct ccagccccgg tccccacccc agccatggtc
     2161 tcagccccgt catccactgt gaatgccagt gcttctgtta agacttctgc aggcaccacc
     2221 gacccagagg aggccacaag gcttctagct gagaagaggc ggctggcccg agagcagaga
     2281 gaaaaggaag aaagggagag gagggagcag gaagagcttg aaagacaaaa gagagaggaa
     2341 ttggctcaac gtgtggctga agagaggacg actcgccgtg aggaggagtc gcgcaggctg
     2401 gaagccgagc aggcccggga gaaggaggag cagctgcagc ggcaggcgga ggagcgggcg
     2461 ctgcgcgagt gggaggaggc agagcgcgcc cagaggcaga aagaagaaga agctcgcgtt
     2521 cgtgaagaag cagagagggt ccggcaggaa cgagagaagc atttccagag agaagagcaa
     2581 gagcgcctgg agagaaagaa gcgacttgag gagattatga aaagaaccag gagaacagaa
     2641 gctacagata agaaaaccag tgatcagaga aacggtgata tagccaaggg agctctcact
     2701 ggaggaacag aggtgtctgc acttccatgt acaacaaacg ctccgggaaa tggaaagcca
     2761 gttggtagcc cacatgtggt tacctcacac cagtcaaaag tgacagtgga gagcactccc
     2821 gatttggaaa aacaaccaaa tgaaaatggt gtatctgttc agaatgaaaa ttttgaagaa
     2881 attataaact tacccattgg atctaaacca tccagattag atgtcaccaa cagtgagagc
     2941 ccagaaattc ctttgaatcc aattttggcc tttgatgatg aagggacact tgggcccctg
     3001 cctcaggtag atggtgttca gacacagcag actgcagaag ttatataatg agcttgtcga
     3061 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3121 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3181 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3241 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3301 tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa
     3361 aggaagctga gttggctgct gccaccgctg agcaataact atcataaccc ctagggtata
     3421 cccatctaat tggaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
     3481 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
     3541 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacaa
     3601 ccggtggagg aaattctcct tgaagtttcc ctggtgttca aagtaaagga gtttgcacca
     3661 gacgcacctc tgttcactgg tccggcgtat taaaacacga tacattgtta ttagtacatt
     3721 tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat
     3781 aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca
     3841 gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga
     3901 ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg
     3961 ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt
     4021 tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt
     4081 ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa tagagcttgg
     4141 acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc
     4201 tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt
     4261 gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat taccggttga
     4321 cttgggtcaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4621 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5161 gttatcccct gattgacttg ggtcgctctt cctgtggatg cgcaggtatg tacaggaaga
     5221 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
     5281 gtttaatcaa ggctctgacg catttctaca accacgactc taagtgtgtg ggtgaagtca
     5341 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
     5401 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
     5461 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
     5521 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
     5581 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
     5641 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
     5701 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
     5761 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
     5821 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
     5881 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
     5941 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
     6001 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
     6061 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
     6121 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga ttgcgcagat
     6181 gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa
     6241 actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta
     6301 tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa
     6361 gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatgtaaa gactatattc
     6421 gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac
     6481 gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc
     6541 caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac
     6601 ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc
     6661 ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg
     6721 ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc
     6781 agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg
     6841 atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg
     6901 catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc
     6961 acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat
     7021 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     7081 cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
     7141 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
     7201 tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt
     7261 tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgccata acttcgtata
     7321 gcatacatta tacgaagtta tctg
//