LOCUS       N-ybbR_MTBD_only_Tau_pO 5478 bp ds-DNA     circular     25-JUN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2522..2962
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(5180..5211)
                     /label="LoxP"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     misc_feature    512..571
                     /label="Primer junction N"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     enhancer        1473..2184
                     /label="1629"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2413..2520)
                     /label="RNAI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(4358..5110)
                     /label="PromGenCDS"
                     /ApEinfo_revcolor=#827cb4
                     /ApEinfo_fwdcolor=#827cb4
     misc_feature    4070..4268
                     /label="Tn7R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     enhancer        3081..4045
                     /label="lef2-603"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1..414
                     /label="ZZ"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     polyA_signal    complement(991..1182)
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1248..1270)
                     /label="T7R (T7 terminator primer)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    505..540
                     /label="ybbR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_recomb     5181..5214
                     /label="LoxP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    541..918
                     /label="Tau MTBD"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    924..931
                     /label="mutiple cloning site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2193..3025)
                     /label="Ori"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     C_region        1306..1472
                     /label="Tn7L"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    415..504
                     /label="Linker TEV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5218..5243
                     /label="I-CeuI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2374..2520
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 ATGTGCatgg caggccttgc gcaacacgat gaagccgtgg acaacaaatt caacaaagaa
       61 caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac
      121 gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct
      181 aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa
      241 aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg aaacgccttc
      301 atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga agctaaaaag
      361 ctaaatgatg ctcaggcgcc gaaagtagac gcaaattccg cggggaagtc aaccgattat
      421 gatattccaa ctactgctag cgagaaccta tatttccagg gaGATTATGA TATCCCTACG
      481 ACTGAGAATC TTTATTTTCA GGGCatgGAC TCATTGGAGT TTATCGCATC AAAGCTCGCA
      541 cgcctgcaga cagcccccgt gcccatgcca gacctgaaga atgtcaagtc caagatcggc
      601 tccactgaga acctgaagca ccagccggga ggcgggaagg tgcagataat taataagaag
      661 ctggatctta gcaacgtcca gtccaagtgt ggctcaaagg ataatatcaa acacgtcccg
      721 ggaggcggca gtgtgcaaat agtctacaaa ccagttgacc tgagcaaggt gacctccaag
      781 tgtggctcat taggcaacat ccatcataaa ccaggaggtg gccaggtgga agtaaaatct
      841 gagaagcttg acttcaagga cagagtccag tcgaagattg ggtccctgga caatatcacc
      901 cacgtccctg gcggaggata atgagcttgt cgagaagtac tagaggatca taatcagcca
      961 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     1021 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     1081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga
     1201 gcctagaaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     1261 ctgagcaata actatcataa cccctagggt atacccatct aattggaacc agataagtga
     1321 aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc
     1381 cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc
     1441 ctcccagttc ccaactattt tgtccgccca caaccggtgg aggaaattct ccttgaagtt
     1501 tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac tggtccggcg
     1561 tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc tgtgcgttgt
     1621 tgatttacag acaattgttg tacgtatttt aataattcat taaatttata atctttaggg
     1681 tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa tttaaatatt
     1741 aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg ttgtttttcc
     1801 gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact tgccaaatct
     1861 tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg
     1921 acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt agtgtacaat
     1981 tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg cgtgttagct
     2041 ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac
     2101 gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa
     2161 tttgtagttg agctttttgg aattaccggt tgacttgggt caactgtcag accaagttta
     2221 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2281 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2341 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2401 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2461 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2521 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2581 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2641 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2701 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2761 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2821 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2881 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2941 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3001 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattgac ttgggtcgct
     3061 cttcctgtgg atgcgcaggt atgtacagga agaggtttat actaaactgt tacattgcaa
     3121 acgtggtttc gtgtgccaag tgtgaaaacc gatgtttaat caaggctctg acgcatttct
     3181 acaaccacga ctctaagtgt gtgggtgaag tcatgcatct tttaatcaaa tcccaagatg
     3241 tgtataaacc accaaactgc caaaaaatga aaactgtcga caagctctgt ccgtttgctg
     3301 gcaactgcaa gggtctcaat cctatttgta attattgaat aataaaacaa ttataaatgt
     3361 caaatttgtt ttttattaac gatacaaacc aaacgcaaca agaacatttg tagtattatc
     3421 tataattgaa aacgcgtagt tataatcgct gaggtaatat ttaaaatcat tttcaaatga
     3481 ttcacagtta atttgcgaca atataatttt attttcacat aaactagacg ccttgtcgtc
     3541 ttcttcttcg tattccttct ctttttcatt tttctcttca taaaaattaa catagttatt
     3601 atcgtatcca tatatgtatc tatcgtatag agtaaatttt ttgttgtcat aaatatatat
     3661 gtctttttta atggggtgta tagtaccgct gcgcatagtt tttctgtaat ttacaacagt
     3721 gctattttct ggtagttctt cggagtgtgt tgctttaatt attaaattta tataatcaat
     3781 gaatttggga tcgtcggttt tgtacaatat gttgccggca tagtacgcag cttcttctag
     3841 ttcaattaca ccatttttta gcagcaccgg attaacataa ctttccaaaa tgttgtacga
     3901 accgttaaac aaaaacagtt cacctccctt ttctatacta ttgtctgcga gcagttgttt
     3961 gttgttaaaa ataacagcca ttgtaatgag acgcacaaac taatatcaca aactggaaat
     4021 gtctatcaat atatagttgc tgattgcgca gatgccctgc gtaagcgggt gtgggcggac
     4081 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     4141 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     4201 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     4261 gggcgtggcc aagggcatgt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa
     4321 ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
     4381 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
     4441 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
     4501 cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
     4561 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
     4621 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
     4681 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
     4741 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
     4801 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
     4861 ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc
     4921 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac
     4981 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     5041 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg
     5101 cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
     5161 tgggcagcag cgaagtcgcc ataacttcgt atagcataca ttatacgaag ttatctgtaa
     5221 ctataacggt cctaaggtag cgagtttaaa cactagtatc gattcgcgac ctactccgga
     5281 atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt
     5341 tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac
     5401 cgtcccacca tcgggcgcgg atctcggtcc gaaaccATGT CGTACTACCA TCACCATCAC
     5461 CATCACcatc acggttct
//