LOCUS       K490_pOmnibac           7806 bp ds-DNA     circular     14-JUN-2020
DEFINITION  .
KEYWORDS    "creator:Luke" "marker:Tn7R"                                       
FEATURES             Location/Qualifiers
     CDS             complement(1601..2353)
                     /label="PromGenCDS"
                     /ApEinfo_revcolor=#827cb4
                     /ApEinfo_fwdcolor=#827cb4
     misc_feature    complement(2423..2454)
                     /label="LoxP"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     CDS             4702..5415
                     /label="GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(join(7242..7806,1..268))
                     /label="Ori"
                     /ApEinfo_revcolor=#ff081d
                     /ApEinfo_fwdcolor=#ff081d
     misc_feature    7570..7570
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    complement(6297..6319)
                     /label="T7R (T7 terminator primer)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5948..6007
                     /label="Second primer junction"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             5422..5967
                     /label="SNAP-tag®"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     enhancer        324..1288
                     /label="lef2-603"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3226..4695
                     /label="K490"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3136..3225
                     /label="Linker TEV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_recomb     2424..2457
                     /label="LoxP"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     misc_feature    1313..1511
                     /label="Tn7R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4435..4695
                     /label="Pred coil coil"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2461..2486
                     /label="I-CeuI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    join(7571..7806,1..205)
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    7423..7569
                     /label="pMB1 ori (from pUC19, no ROP)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     C_region        6355..6521
                     /label="Tn7L"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5973..5980
                     /label="mutiple cloning site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     polyA_signal    complement(6040..6231)
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     enhancer        6522..7233
                     /label="1629"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(7462..7569)
                     /label="RNAI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2722..3135
                     /label="ZZ"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
ORIGIN
        1 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
       61 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      121 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      181 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      241 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt gacttgggtc
      301 gctcttcctg tggatgcgca ggtatgtaca ggaagaggtt tatactaaac tgttacattg
      361 caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt
      421 tctacaacca cgactctaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag
      481 atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg
      541 ctggcaactg caagggtctc aatcctattt gtaattattg aataataaaa caattataaa
      601 tgtcaaattt gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt
      661 atctataatt gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa
      721 tgattcacag ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc
      781 gtcttcttct tcgtattcct tctctttttc atttttctct tcataaaaat taacatagtt
      841 attatcgtat ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata
      901 tatgtctttt ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac
      961 agtgctattt tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc
     1021 aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc
     1081 tagttcaatt acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta
     1141 cgaaccgtta aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg
     1201 tttgttgtta aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga
     1261 aatgtctatc aatatatagt tgctgattgc gcagatgccc tgcgtaagcg ggtgtgggcg
     1321 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     1381 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     1441 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     1501 gaggggcgtg gccaagggca tgtaaagact atattcgcgg cgttgtgaca atttaccgaa
     1561 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     1621 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     1681 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     1741 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     1801 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     1861 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     1921 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     1981 tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     2041 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     2101 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
     2161 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     2221 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     2281 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     2341 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     2401 ccttgggcag cagcgaagtc gccataactt cgtatagcat acattatacg aagttatctg
     2461 taactataac ggtcctaagg tagcgagttt aaacactagt atcgattcgc gacctactcc
     2521 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     2581 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     2641 taccgtccca ccatcgggcg cggatctcgg tccgaaaccA TGTCGTACTA CCATCACCAT
     2701 CACCATCACc atcacggttc tATGTGCatg gcaggccttg cgcaacacga tgaagccgtg
     2761 gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac
     2821 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
     2881 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac
     2941 aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac
     3001 gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac
     3061 cttttagcag aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc
     3121 gcggggaagt caaccgatta tgatattcca actactgcta gcgagaacct atatttccag
     3181 ggaGATTATG ATATCCCTAC GACTGAGAAT CTTTATTTTC AGGGCATGGC GGACCTGGCC
     3241 GAGTGCAACA TCAAAGTGAT GTGTCGCTTC AGACCTCTCA ACGAGTCTGA AGTGAACCGC
     3301 GGCGACAAGT ACATCGCCAA GTTTCAGGGA GAAGACACGG TCGTGATCGC GTCCAAGCCT
     3361 TATGCATTTG ATCGGGTGTT CCAGTCAAGC ACATCTCAAG AGCAAGTGTA TAATGACTGT
     3421 GCAAAGAAGA TTGTTAAAGA TGTACTTGAA GGATATAATG GAACAATATT TGCATATGGA
     3481 CAAACATCCT CTGGGAAGAC ACACACAATG GAGGGTAAAC TTCATGATCC AGAAGGCATG
     3541 GGAATTATTC CAAGAATAGT GCAAGATATT TTTAATTATA TTTACTCCAT GGATGAAAAT
     3601 TTGGAATTTC ATATTAAGGT TTCATATTTT GAAATATATT TGGATAAGAT AAGGGACCTG
     3661 TTAGATGTTT CAAAGACCAA CCTTTCAGTT CATGAAGACA AAAACCGAGT TCCCTATGTA
     3721 AAGGGGTGCA CAGAGCGTTT TGTATGTAGT CCAGATGAAG TTATGGATAC CATAGATGAA
     3781 GGAAAATCCA ACAGACATGT AGCAGTTACA AATATGAATG AACATAGCTC TAGGAGTCAC
     3841 AGTATATTTC TTATTAATGT CAAACAAGAG AACACACAAA CGGAACAAAA GCTGAGTGGA
     3901 AAACTTTATC TGGTTGATTT AGCTGGTAGT GAAAAGGTTA GTAAAACTGG AGCTGAAGGT
     3961 GCTGTGCTGG ATGAAGCTAA AAACATCAAC AAGTCACTTT CTGCTCTTGG AAATGTTATT
     4021 TCTGCTTTGG CTGAGGGTAG TACATATGTT CCATATCGAG ATAGTAAAAT GACAAGAATC
     4081 CTTCAAGATT CATTAGGTGG CAACTGTAGA ACCACTATTG TAATTTGCTG CTCTCCATCA
     4141 TCATACAATG AGTCTGAAAC AAAATCTACA CTCTTATTTG GCCAAAGGGC CAAAACAATT
     4201 AAGAACACAG TTTGTGTCAA TGTGGAGTTA ACTGCAGAAC AGTGGAAAAA GAAGTATGAA
     4261 AAAGAAAAAG AAAAAAATAA GATCCTGCGG AACACTATTC AGTGGCTTGA AAATGAGCTC
     4321 AACAGATGGC GTAATGGGGA GACGGTGCCT ATTGATGAAC AGTTTGACAA AGAGAAAGCC
     4381 AACTTGGAAG CTTTCACAGT GGATAAAGAT ATTACTCTTA CCAATGATAA ACCAGCAACC
     4441 GCAATTGGAG TTATAGGAAA TTTTACTGAT GCTGAAAGAA GAAAGTGTGA AGAAGAAATT
     4501 GCTAAATTAT ACAAACAGCT TGATGACAAG GATGAAGAAA TTAACCAGCA AAGTCAACTG
     4561 GTAGAGAAAC TGAAGACGCA AATGTTGGAT CAGGAGGAGC TTTTGGCATC TACCAGAAGG
     4621 GATCAAGACA ATATGCAAGC TGAGCTGAAT CGCCTTCAAG CAGAAAATGA TGCCTCTAAA
     4681 GAAGAAGTGA AAGAAGGTTC Tatgagtaaa ggagaagaac ttttcactgg agttgtccca
     4741 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     4801 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     4861 cctgttccat ggccaacact tgtcactact ctgacttatg gtgttcaatg cttttcaaga
     4921 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     4981 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     5041 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     5101 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     5161 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     5221 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     5281 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     5341 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     5401 gatgaactat acaaaggttc tatggacaaa gactgcgaaa tgaagcgcac caccctggat
     5461 agccctctgg gcaagctgga actgtctggg tgcgaacagg gcctgcaccg tatcatcttc
     5521 ctgggcaaag gaacatctgc cgccgacgcc gtggaagtgc ctgccccagc cgccgtgctg
     5581 ggcggaccag agccactgat gcaggccacc gcctggctca acgcctactt tcaccagcct
     5641 gaggccatcg aggagttccc tgtgccagcc ctgcaccacc cagtgttcca gcaggagagc
     5701 tttacccgcc aggtgctgtg gaaactgctg aaagtggtga agttcggaga ggtcatcagc
     5761 tacagccacc tggccgccct ggccggcaat cccgccgcca ccgccgccgt gaaaaccgcc
     5821 ctgagcggaa atcccgtgcc cattctgatc ccctgccacc gggtggtgca gggcgacctg
     5881 gacgtggggg gctacgaggg cgggctcgcc gtgaaagagt ggctgctggc ccacgagggc
     5941 cacagactgg gcaagcctgg gctgggttaa tgagcttgtc gagaagtact agaggatcat
     6001 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     6061 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     6121 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6181 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc
     6241 actgcttgag cctagaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     6301 ctgccaccgc tgagcaataa ctatcataac ccctagggta tacccatcta attggaacca
     6361 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
     6421 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
     6481 tttccacccc tcccagttcc caactatttt gtccgcccac aaccggtgga ggaaattctc
     6541 cttgaagttt ccctggtgtt caaagtaaag gagtttgcac cagacgcacc tctgttcact
     6601 ggtccggcgt attaaaacac gatacattgt tattagtaca tttattaagc gctagattct
     6661 gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
     6721 tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
     6781 ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
     6841 tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt
     6901 gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat
     6961 aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta
     7021 gtgtacaatt gactcgacgt aaacacgtta aatagagctt ggacatattt aacatcgggc
     7081 gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct
     7141 tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc
     7201 gcgatcaaat ttgtagttga gctttttgga attaccggtt gacttgggtc aactgtcaga
     7261 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7321 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7561 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7801 cctaca
//