LOCUS       pCFJ1359___Intro        3169 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     308 bases. 199 bases in phase. PATC density = 86
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(1667..1684)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     858..878
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      48..730
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2460..3119
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1043..1053
                     /locus_tag="Goldgen Gate 2A"
                     /label="Goldgen Gate 2A"
                     /ApEinfo_label="Goldgen Gate 2A"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1354..1364
                     /locus_tag="Golden Gate 2B"
                     /label="Golden Gate 2B"
                     /ApEinfo_label="Golden Gate 2B"
                     /ApEinfo_fwdcolor="#66ccff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1050..1357
                     /locus_tag="smu-1 intron (308 bases. 199 bases in phase.
                     PATC density = 86)"
                     /label="smu-1 intron (308 bases. 199 bases in phase. PATC
                     density = 86)"
                     /ApEinfo_label="smu-1 intron (308 bases. 199 bases in
                     phase. PATC density = 86)"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
       61 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      241 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
      301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      721 caacgcggcc tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc
      781 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccag
      841 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tatcaacttt
      901 gtatagaaaa gttgGTGAAT TGTAATACGA CTCACTATAG GGCGAATTaa ttCGAGCTCG
      961 GTACCcagct tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcagca
     1021 agtttgtaca aaaaagcagg ctggtctcaa tgtctacctg cctgcctacc gcctaaattt
     1081 tttgtgaagt ttcttcaaaa aatccagaaa aaaaaacaat tttcatacga ttttttccct
     1141 taaaattgtg aattttcatg ctttttagcc ccaaaagtca ttatttgaga aaaaatttca
     1201 tacaaaaaag ttttgagaaa tacacaattt tttaaatgta attttcaaat tttcaatttt
     1261 caactagaaa attcacaaaa cttgtaaatt ttggaccaaa acatttatac aattactttt
     1321 tttgaatcta ataactacaa taactacaaa tttaacaaga gaccacccag ctttcttgta
     1381 caaagtggac cagcctcaag aacacccgaa tggagtctct aagctacata ataccaactt
     1441 acactttaca aaatgttgtc ccccaaaatg tagccattcg tatctgctcc taataaaaag
     1501 aaagtttctt cacattctaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaac cccccccccc
     1561 cctgcaggca tgcattaatt aaaggccatg atggccagct agcttgagta ttcTATAGTG
     1621 TCACCTAAAT AGCTTGGCca actttattat acatagttga taattcactg gccgtcgttt
     1681 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1741 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1801 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     1861 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     1921 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     1981 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     2041 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     2101 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     2161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2461 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2521 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2581 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2641 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2701 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2761 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2821 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2881 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2941 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3001 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3061 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaact 
//
